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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu / Tam, Joseph

CiteScore 2016: 1.01

SCImago Journal Rank (SJR) 2016: 0.349
Source Normalized Impact per Paper (SNIP) 2016: 0.495

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Volume 25, Issue 2


Anti-inflammatory and analgesic activities of the methanol leaf extract of Phyllanthus amarus in some laboratory animals

Sunday Oluwaseun Ofuegbe
  • Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Adeolu Alex Adedapo
  • Corresponding author
  • Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Abiodun Adegoke Adeyemi
Published Online: 2013-07-26 | DOI: https://doi.org/10.1515/jbcpp-2013-0084


Background: Phyllanthus amarus is used in Nigeria and other parts of the world as a medicinal plant. The plant has a history of use in Ayurvedic medicine for over 2000 years as well as a wide variety of traditional applications. It is being evaluated for anti-inflammatory and analgesic potential because these phenomena have assumed a worldwide dimension as a result of their implications in a variety of diseases.

Methods: The methanol extract of the leaves of P. amarus was investigated for its anti-inflammatory and analgesic activities in Wistar strain albino rats and mice using carrageenan-induced inflammation as well as histamine-induced edema. The analgesic effect was determined using acetic acid writhing method and formalin-induced paw lick test. Standard phytochemical analysis was carried out to determine the active constituents responsible for biological effects.

Results: The extract at 100 and 200 mg/kg reduced significantly (p<0.05) the formation of edema induced by histamine and carrageenan. In the acetic acid-induced writhing test, the extract showed good analgesic activity characterized by a significant reduction in the number of writhes with 100 and 200 mg/kg doses used when compared to the control group. In the formalin-induced paw lick test, the extract at 100 and 200 mg/kg doses exhibited good analgesic activity characterized by a significant reduction in the number of paw licks at both the early and the late phases of the induced pain when compared with the untreated control group. Ibuprofen at 10 mg/kg served as the reference drug in all these tests.

Conclusions: Results suggested that methanol extract of the leaves of P. amarus has great anti-inflammatory and analgesic potential. These biological effects exhibited by the extract of this plant may be attributed to the presence of flavonoids and other phenols contained therein.

Keywords: analgesic; anti-inflammatory; edema; mice; Phyllanthus amarus; rats


  • 1.

    Perianayagam JB, Sharma SK, Pillai KK. Anti-inflammatory activity of Trichodesma indicum root extract in experimental animal. J Ethnopharmacol 2006;104:410–4.CrossrefPubMedGoogle Scholar

  • 2.

    Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1β generation. Clin Exp Immunol 2007;147:611–27.Web of ScienceGoogle Scholar

  • 3.

    Dormandy, T. The worst of evils: man’s fight against pain. New Haven, CT: Yale University Press, 2006:22.Google Scholar

  • 4.

    Adedapo AA, Ofuegbe SO. Anti-inflammatory and analgesic activities of soft drink leaf extract of Phyllanthus amarus in some laboratory animals. Br Biotechnol J 2013;3:191–204.Google Scholar

  • 5.

    Kumara NK. Identification of strategies to improve research on medicinal plants used in Sri Lanka. In: WHO Symposium. Galle, Sri Lanka: University of Ruhuna, 2001.Google Scholar

  • 6.

    Dharmasiri JR, Jayakody AC, Galhena G, Liyanage SS, Ratnasooriya WD. Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo. J Ethnopharmacol 2003;87:199–206.CrossrefPubMedGoogle Scholar

  • 7.

    Li RW Myers SP, Leach DN, Lin GD, Leach G. A cross-cultural study: anti-inflammatory activity of Australian and Chinese plants. J Ethnopharmacol 2003;85:25–32.CrossrefPubMedGoogle Scholar

  • 8.

    Adedapo AA, Adegbayibi AY, Emikpe BO. Some clinico-pathological changes associated with the aqueous extract of the leaves of Phyllanthus amarus in rats. Phytother Res 2005;19:971–6.PubMedCrossrefGoogle Scholar

  • 9.

    Odetola AA, Akojenu SM. Antidiarrhoeal and gastrointestinal potentials of the aqueous extracts of Phyllanthus amarus (Euphorbiaceae). Afr J Med Sci 2000;29:119–22.Google Scholar

  • 10.

    Adeneye AA, Benebo AS, Agbaje EO. Protective effect of the aqueous leaf and seed extract of Phyllanthus amarus on alcohol-induced hepatotoxicity in rats. West Afr J Pharmacol Drug Res 2006;22–23:42–50.Google Scholar

  • 11.

    Subeki S, Matsuura H, Takahashi K, Yamasaki M, Yamato O, Maede Y, et al. Anti-babesial and anti-plasmodial compounds from Phyllanthus niruri. J Nat Prod 2005;68:537–9.PubMedCrossrefGoogle Scholar

  • 12.

    Adedapo AA, Koduru S, Jimoh FO, Masika PJ, Afolayan AJ. Evaluation of the medicinal potentials of the methanol extracts of the leaves and stem of Halleria lucida. Bioresour Technol 2008;99:4158–63.CrossrefWeb of SciencePubMedGoogle Scholar

  • 13.

    Ayoola GA, Coker HA, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC, et al. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Trop J Pharm Res 2008;7:1019–24.Google Scholar

  • 14.

    Gupta M, Mazunder UK, Sambath KR, Gomath P, Rajeshwar Y, Kakoti BB. Anti-inflammatory, analgesic and antipyretic effects of methanol extract from Bauhina racemosa stem bark in animal models. J Ethnopharmacol 2005;98:267–73.CrossrefGoogle Scholar

  • 15.

    Sawadogo WR, Boly R, Lompo M, Some N, Lamien CE, Guissouu IP, et al. Anti-inflammatory, analgesic and antipyretic activities of Dicliptera verticillata. Int J Pharmacol 2006;2:435–8.Google Scholar

  • 16.

    Moody JO, Robert VA, Connolly JD, Houghton PJ. Anti-inflammatory activities of the methanol extracts and an isolated furanoditerpene constituent of Sphenocentrum jollyanum Pierre (Menispermaceae). J Ethnopharmacol 2006;104;87–91.CrossrefPubMedGoogle Scholar

  • 17.

    Michelle V, William M, Charles J, Alan R. Estrous cycle modulation of nociceptive behaviours elicited by electrical and formalin. Pharmacol Biochem Behav 2001;69:315–24.Google Scholar

  • 18.

    Asongalem E, Foyet H, Ekoo S, Dimo T, Kamtchouing P. Antiinflammatory, lack of central analgesia and antipyretic properties of Acanthus montanus (Ness) T. Anderson. J Ethnopharmacol 2004;95:63–8.PubMedCrossrefGoogle Scholar

  • 19.

    Linardi A, Costa SK, DeSilva GR, Antunes E. Involvement of kinins, mast cells and sensory neurons in the plasma exudation and paw oedema induced by staphylococcal enterotoxin B in the mouse. Eur J Pharmacol 2002;399:325–42.Google Scholar

  • 20.

    Okoli CO, Ibiam FA, Ezike AC, Akah PA, Okoye TC. Evaluation of antidiabetic potentials of Phyllanthus niruri in alloxan diabetic rats. Afr J Biotechnol 2010;9:248–59.Google Scholar

  • 21.

    Vasudevan M, Gunman KK, Parle M. Antinociceptive and anti-inflammatory effects of Thespesia populnea bark extract. J Ethnopharmacol 2007;109:264–70.CrossrefPubMedGoogle Scholar

  • 22.

    Ghannadi A, Hajhashemi V, Jafarabadi H. An investigation of the analgesic and anti-inflammatory effects of Nigella sativa seed polyphenols. J Med Food 2005;8:488–93.CrossrefPubMedWeb of ScienceGoogle Scholar

  • 23.

    La Casa C, Villegas I, Aarcon de la Lastra C, Motilva V, Martin Calero MJ. Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions. J Ethnopharmacol 2000;71:45–53.CrossrefGoogle Scholar

  • 24.

    Calixto JB, Beirith A, Ferreira J, Santos AR, Cechinel Filho V, Yunes RA. Naturally occurring antinociceptive substances from plants. Phytother Res 2000;14:401–18.PubMedCrossrefGoogle Scholar

  • 25.

    Dhara AK, Suba V, Sen T, Pal S, Chaudhuri AK. Preliminary studies on the anti-inflammatory and analgesic activity of the methanol fraction of the root extract of Tragia involucrata Linn. J Ethnopharmacol 2000;72:265–8.PubMedCrossrefGoogle Scholar

About the article

Corresponding author: Adeolu Alex Adedapo, Faculty of Veterinary Medicine, Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Oyo State 20005, Nigeria, Phone: +234 816 2746 222, E-mail:

Received: 2013-06-25

Accepted: 2013-07-02

Published Online: 2013-07-26

Published in Print: 2014-05-01

Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, Volume 25, Issue 2, Pages 175–180, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2013-0084.

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