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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Haim, Abraham / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu

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2191-0286
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Volume 25, Issue 4

Issues

Analgesic, anti-inflammatory and antipyretic effects of Ixora coccinea

Md. Syed Ali Adnan / Md. Mamun Al-Amin / Mir Muhammad Nasir Uddin / M. Shohel / Rajib Bhattacharjee / J.M.A. Hannan / Biplab Kumar Das
  • Faculty of Pharmacy, Department of Pharmaceutical Chemistry, University of Dhaka, Dhaka, Bangladesh
  • Other articles by this author:
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Published Online: 2014-01-27 | DOI: https://doi.org/10.1515/jbcpp-2013-0125

Abstract

Background: The present study was carried out to explore the potential of the ethanol extract of Ixora coccinea L. (IC) leaves as analgesic, anti-inflammatory and antipyretic agents using the hot-plate, acetic acid-induced writhing, carrageenan-induced paw edema and brewer’s yeast-induced pyrexia tests in rodents.

Methods: The extract was prepared by soaking the dried powdered leaves of IC in ethanol for 2 days. The filtrate thus obtained by filtration and evaporation was considered as a stock solution and was used in all experimental models.

Results: Oral administration of IC (250 and 500 mg/kg) significantly (p<0.05) increased the reaction time in the hot-plate test. Ixora coccinea (250 and 500 mg/kg) produced 56.14% and 63.16% inhibition (p<0.05) in acetic acid-induced writhing. It also (250 and 500 mg/kg) produced significant (p<0.05) inhibition of paw edema pronounced at 6 h after carrageenan injection. Intraperitoneal administration of IC (250 and 500 mg/kg) lowered the body temperature in brewer’s yeast-induced hyperthermia.

Conclusions: Based on the findings, it may be concluded that the IC leaves possessed analgesic, anti-inflammatory, and antipyretic activities. Phytochemical constituents of IC leaves such as flavonoids, tannins, and triterpenes in ethanol extract could be correlated with its observed biological activities.

Keywords: biological activity; ethanol extract; inflammation

References

  • 1.

    Upaganlawar A, Tende C, Yeole P. Antiinflammatory activity of aqueous extract of Oroxylum indicum vent. leaves extract-preliminary study. Pharmacol Online 2009;1:22–6.Google Scholar

  • 2.

    Zaveri M, Gohil P, Jain S. Immunostimulant activity of n-butanol fraction of root bark of Oroxylum indicum vent. J Immunotoxicol 2006;3:83–99.CrossrefPubMedGoogle Scholar

  • 3.

    Kawsar U, Abu S, Anwarul I, Aziz Abdur R, Seatara K, Khan GR, et al. Biological activities of extracts and two flavonoids from Oroxylum indicum vent. (Bignoniaceae). J Biol Sci 2003;3:371–5.Google Scholar

  • 4.

    Meena C, Rajesh K, Radhika L, Rosamma M, Peter S. Phytochemical and antimicrobial study of Oroxylum indicum. Anc Sci Life 2011;30:114–20.PubMedGoogle Scholar

  • 5.

    Vyas B, Shah D, Shah P, Shah S, Joshi S, Gandhi T. Protective effect of aqueous extract of Oroxylum indicum Linn. (root bark) against DNBS-induced colitis in rats. Indian J Pharmacol 2011;43:656–61.PubMedGoogle Scholar

  • 6.

    Rylski M, Duriasz-Rowinska H, Rewerski W. The analgesic action of some flavonoids in the hot plate test. Acta Physiol Pol 1979;30:385–8.PubMedGoogle Scholar

  • 7.

    Kitchen I, Crowder M. Assessment of the hot-plate antinociceptive test in mice. A new method for the statistical treatment of graded data. J Pharmacol Methods 1985;13:1–7.PubMedCrossrefGoogle Scholar

  • 8.

    Gawade S. Acetic acid induced painful endogenous infliction in writhing test on mice. J Pharmacol Pharmacother 2012;3:348.CrossrefPubMedGoogle Scholar

  • 9.

    Roy MK, Nakahara K, Na TV, Trakoontivakorn G, Takenaka M, Isobe S, et al. Baicalein, a flavonoid extracted from a methanolic extract of Oroxylum indicum inhibits proliferation of a cancer cell line in vitro via induction of apoptosis. Die Pharmazie 2007;62:149–53.PubMedGoogle Scholar

  • 10.

    Moirangthem DS, Talukdar NC, Bora U, Kasoju N, Das RK. Differential effects of Oroxylum indicum bark extracts: antioxidant, antimicrobial, cytotoxic and apoptotic study. Cytotechnology 2013;65:83–95.PubMedCrossrefWeb of ScienceGoogle Scholar

  • 11.

    Laupattarakasem P, Houghton PJ, Hoult JR, Itharat A. An evaluation of the activity related to inflammation of four plants used in Thailand to treat arthritis. J Ethnopharmacol 2003;85:207–15.PubMedCrossrefGoogle Scholar

  • 12.

    Bhakthavatsalam P, Kamatchi GL, Ghosh MN. Dissociation of receptor sensitivity changes in rat perifornical hypothalamus: a role for dopaminergic receptors in amphetamine anorexic tolerance. J Pharmacol Exp Ther 1987;240:196–202.PubMedGoogle Scholar

  • 13.

    Momin FN, Kalai BR, Shikalgar TS, Naikwade NS. Cardioprotective effect of methanolic extract of Ixora coccinea Linn. leaves on doxorubicin-induced cardiac toxicity in rats. Indian J Pharmacol 2012;44:178–83.PubMedWeb of ScienceCrossrefGoogle Scholar

  • 14.

    Annapurna J, Amarnath PV, Amar Kumar D, Ramakrishna SV, Raghavan KV. Antimicrobial activity of Ixora coccinea leaves. Fitoterapia 2003;74:291–3.PubMedCrossrefGoogle Scholar

  • 15.

    Idowu TO, Ogundaini AO, Salau AO, Obuotor EM, Bezabih M, Abegaz BM. Doubly linked, A-type proanthocyanidin trimer and other constituents of Ixora coccinea leaves and their antioxidant and antibacterial properties. Phytochemistry 2010;71:2092–8.PubMedCrossrefWeb of ScienceGoogle Scholar

  • 16.

    Deraedt R, Jouquey S, Delevallee F, Flahaut M. Release of prostaglandins E and F in an algogenic reaction and its inhibition. Eur J Pharmacol 1980;61:17–24.CrossrefGoogle Scholar

  • 17.

    Ratnasooriya WD, Deraniyagala SA, Bathige SD, Goonasekara CL, Jayakody JR. Antinociceptive action of aqueous extract of the leaves of Ixora coccinea. Acta Biol Hungarica 2005;56:21–34.CrossrefGoogle Scholar

  • 18.

    Lanhers MC, Fleurentin J, Mortier F, Vinche A, Younos C. Anti-inflammatory and analgesic effects of an aqueous extract of Harpagophytum procumbens. Planta Med 1992;58:117–23.CrossrefPubMedGoogle Scholar

  • 19.

    Williamson EM, Okpako DT, Evans FJ. Selection, preparation and pharmacological evaluation of plant materials. In: Pharmacological methods in phytotherapy research, Vol. 1. Chichester: John Wiley, 1996:184–6.Google Scholar

  • 20.

    Rates SM. Plants as source of drugs. Toxicon 2001;39:603–13.CrossrefPubMedGoogle Scholar

  • 21.

    Bentley GA, Newton SH, Starr J. Studies on the antinociceptive action of alpha-agonist drugs and their interactions with opioid mechanisms. Br J Pharmacol 1983;79:125–34.PubMedCrossrefGoogle Scholar

  • 22.

    Dharmasiri MG, Ratnasooriya WD, Thabrew MI. Water extract of leaves and stems of preflowering but not flowering plants of Anisomeles indica possesses analgesic and antihyperalgesic activities in rats. Pharm Biol 2003;41:37–44.CrossrefGoogle Scholar

  • 23.

    Lokuhetty D, Abeywardene E, Perera K, Gunatilake M, Gunapala N, Katulanda P, et al. Effects of Cinnamomum zeylanicum (Ceylon cinnamon) on blood glucose and lipids in a diabetic and healthy rat model. Pharmacognosy Res 2012;4:73–9.Google Scholar

  • 24.

    Chen YF, Tsai HY, Wu TS. Anti-inflammatory and analgesic activities from roots of Angelica pubescens. Planta Med 1995;61:2–8.PubMedCrossrefGoogle Scholar

  • 25.

    Winter CA, Risley EA, Nuss GW. Carrageenan-induced edema in hind paw of the rat as an assay for antiiflammatory drugs. Proc Soc Exp Biol Med Soc Exp Biol Med (New York, NY) 1962;111:544–7.CrossrefGoogle Scholar

  • 26.

    Morris CJ. Carrageenan-induced paw edema in the rat and mouse. Methods Mol Biol 2003;225:115–21.PubMedGoogle Scholar

  • 27.

    Damas J, Remacle-Volon G, Deflandre E. Further studies of the mechanism of counter irritation by turpentine. Naunyn-Schmiedeberg’s Arch Pharmacol 1986;332:196–200.Google Scholar

  • 28.

    Uzcategui B, Avila D, Suarez-Roca H, Quintero L, Ortega J, Gonzalez B. Anti-inflammatory, antinociceptive, and antipyretic effects of Lantana trifolia Linnaeus in experimental animals. Invest Clin 2004;45:317–22.PubMedGoogle Scholar

  • 29.

    Zakaria ZA, Mustapha S, Sulaiman MR, Mat Jais AM, Somchit MN, Abdullah FC. The antinociceptive action of aqueous extract from Muntingia calabura leaves: the role of opioid receptors. Med Principles Pract Int J Kuwait Univ Health Sci Centre 2007;16:130–6.CrossrefGoogle Scholar

  • 30.

    Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci 2004;96:229–45.CrossrefPubMedGoogle Scholar

  • 31.

    Ramesh M, Rao YN, Rao AV, Prabhakar MC, Rao CS, Muralidhar N, et al. Antinociceptive and anti-inflammatory activity of a flavonoid isolated from Caralluma attenuata. J Ethnopharmacol 1998;62:63–6.PubMedCrossrefGoogle Scholar

  • 32.

    Beirith A, Santos AR, Calixto JB, Hess SC, Messana I, Ferrari F, et al. Study of the antinociceptive action of the ethanolic extract and the triterpene 24-hydroxytormentic acid isolated from the stem bark of Ocotea suaveolens. Planta Med 1999;65:50–5.Google Scholar

  • 33.

    Veluchamy, Ghosh D, Thejomoorthy P. Anti-inflammatory and analgesic activities of oleanolic acid 3-/3- Glucoside (RDG-1) from Randia dumetorum (Rubiaceae). Indian J Pharmacol 1983;15:331–42.Google Scholar

  • 34.

    Starec M, Waitzova D, Elis J. [Evaluation of the analgesic effect of RG-tannin using the “hot plate” and “tail flick” method in mice]. Cesk Farm 1988;37:319–21. Epub 1988/09/01. Hodnoceni analgetickeho ucinku RG-tanninu metodou “horka deska” a “tail-flick” u mysi.Google Scholar

  • 35.

    Cragg GM, Newman DJ. Natural product drug discovery in the next millennium. Pharm Biol 2001;39:8–17.PubMedCrossrefGoogle Scholar

  • 36.

    Modak M, Dixit P, Londhe J, Ghaskadbi S, Devasagayam TP. Recent advances in Indian herbal drug research. Guest Editor: Thomas Paul, Asir Devasagayam, Indian Herbs and Herbal Drugs Used for the Treatment of Diabetes. J Clin Biochem Nutr 2007;40:163–73.CrossrefGoogle Scholar

  • 37.

    Paridhavi K, Udupa N, Mallikarjuna R, Mutalik S. Antipyretic and analgesic effect of leaves of Solanum Melongena Linn. in rodents. Indian J Pharmacol 2003;35:312–5.Google Scholar

  • 38.

    Brasseur T. [Anti-inflammatory properties of flavonoids]. J Pharm Belgique 1989;44:235–41. Epub 1989/05/01. Proprietes anti-inflammatoires de flavonoides.Google Scholar

  • 39.

    Vimala R, Nagarajan S, Alam M, Susan T, Joy S. Antiinflammatory and antipyretic activity of Michelia champaca Linn., (white variety), Ixora brachiata Roxb. and Rhynchosia cana (Willd.) D.C. flower extract. Indian J Exp Biol 1997;35:1310–4.PubMedGoogle Scholar

  • 40.

    Chaudhary AK, Harminder, Singh V. A review on the taxonomy, ethnobotany, chemistry and pharmacology of Oroxylum indicum vent. Indian J Pharm Sci 2011;73:483–90.Web of ScienceCrossrefPubMedGoogle Scholar

  • 41.

    Handunetti SM, Kumara RR, Deraniyagala SA, Ratnasooriya WD. Anti-inflammatory activity of Ixora coccinea methanolic leaf extract. Phcog Res 2009;1:80–90.Google Scholar

About the article

Corresponding author: M.M. Al-Amin, Department of Pharmacy, North South University, Plot 15, Block B, Bashundhara, Dhaka-1229, Bangladesh, Phone: +88-01927077102, Fax: +88-2-8852016, E-mail: ;


Received: 2013-08-20

Accepted: 2013-12-09

Published Online: 2014-01-27

Published in Print: 2014-11-01


Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, Volume 25, Issue 4, Pages 423–428, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2013-0125.

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