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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu / Tam, Joseph


CiteScore 2016: 1.01

SCImago Journal Rank (SJR) 2016: 0.349
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2191-0286
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Volume 26, Issue 3

Issues

Modulatory effects of cod liver oil on the antioxidant status and oxidative stress induced by acute exposure to carbon tetrachloride (CCL4) in experimental animal models

Abam Esther Omugba
  • Department of Chemical Sciences, Biochemistry Option, Bells University of Technology, Ota, Nigeria
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/ Adebayo J. Ajiboye
  • Department of Chemical Sciences, Biochemistry Option, Bells University of Technology, Ota, Nigeria
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/ Ademola A. Oyagbemi
  • Corresponding author
  • Faculty of Veterinary Medicine, Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan 200005, Nigeria
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/ Eze Agofure
  • Department of Chemical Sciences, Biochemistry Option, Bells University of Technology, Ota, Nigeria
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Published Online: 2014-05-29 | DOI: https://doi.org/10.1515/jbcpp-2013-0143

Abstract

Background: This work was designed to investigate the modulatory effects of cod liver oil on the acute exposure to carbon tetrachloride (CCL4) in experimental animal models.

Methods: Markers of oxidative stress, enzymic and non-enzymic antioxidants were assessed in the liver and kidney.

Results: The results showed significant (p<0.05) increase in the total protein of the kidney of rats pretreated with cod liver oil compared to the control and CCL4-treated groups. Acute exposure to CCL4 also significantly (p<0.05) increased thiobarbituric acid reactive substances in the liver of rats treated with cod liver oil compared to the CCL4-treated rats. The reduced glutathione (GSH) content in the liver of the rats administered with CCL4 decreased significantly compared to that in all other treatment groups, whereas rats pretreated and co-administered with cod liver oil and CCl4 showed significant (p<0.05) improvement in the liver GSH content. The catalase (CAT) activity in the liver of rats exposed to CCL4 decreased (p<0.05) significantly, whereas the activity increased (p<0.05) significantly in the rats that were treated with cod liver oil compared to the CCl4 only-treated group. The percentage inhibition of superoxide dismutase (SOD) increased (p<0.05) significantly in the liver of rats administered cod liver oil compared to the CCL4-treated rats and the control.

Conclusions: Taken together, cod liver oil attenuates CCL4-induced toxicity and oxidative stress in the liver but not in the kidney of rats acutely exposed to CCL4.

Keywords: antioxidant; carbon tetrachloride (CCL4); cod liver oil; oxidative stress

References

  • 1.

    Jia R, Cao L, Du J, Xu P, Jeney G, Yin G. The protective effect of silymarin on the carbon tetrachloride (CCl4)-induced liver injury in common carp (Cyprinus carpio). In Vitro Cell Dev Biol Anim 2013;49:155–61.Web of ScienceGoogle Scholar

  • 2.

    Zhang S, Lu B, Han X, Xu L, Qi Y, Yin L, et al. Protection of the flavonoid fraction from Rosa laevigata Michx fruit against carbon tetrachloride-induced acute liver injury in mice. Food Chem Toxicol 2013;55:60–9.Web of ScienceGoogle Scholar

  • 3.

    Weber LW, Boll M, Stampfl A. Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Crit Rev Toxicol 2003;33:105–36.Google Scholar

  • 4.

    Naqshbandi A, Khan MW, Rizwan S, Yusufi AN, Khan F. Studies on the protective effect of fish oil against cisplatin induced hepatotoxicity. Biol Med 2011;3:86–97.Google Scholar

  • 5.

    Kalra J, Ali B, Kalra S, Pant KK. Fish oil and its role in acetaminophen induced hepatic injury. Asian J Exp Biol Sci 2012;4:826–9.Google Scholar

  • 6.

    Sekine S, Sasanuki S, Murana Y, Aoyama T, Takeuchi H. Alpha-linolenic acid-rich flaxseed oil ingestion increases plasma adiponectin level in rats. Int J Vitam Nutr Res 2008;78:223–9.Web of ScienceGoogle Scholar

  • 7.

    Mohideen S, Ilavarasan R, Sasikala E, Thirumalaikumarn R. Hepatoprotective activity of Nigellasativa Linn. Ind J Pharm Sci 2003;65:550–1.Google Scholar

  • 8.

    Clairborne A. Catalase activity. In: Greewald AR, editor. Handbook of methods for oxygen radical research. Boca Raton, FL: CRC Press, 1995:237–42.Google Scholar

  • 9.

    Misra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972;247:3170–5.Google Scholar

  • 10.

    Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR. Bromobenzene induced liver necrosis: protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 1974;11:151–69.PubMedCrossrefGoogle Scholar

  • 11.

    Farombi EO, Tahnteng JG, Agboola AO, Nwankwo JO, Emerole GO. Chemoprevention of 2-acetylaminofluorene-induced hepatotoxicity and lipid peroxidation in rats by kolaviron – a Garcinia kola seed extract. Food Chem Toxicol 2000;38:535–41.Google Scholar

  • 12.

    Reitmann S, Frankel S. Colorirnetric method for the determination of serum transaminase activity. Am J Clin Pathol 1957;28:56–68.Google Scholar

  • 13.

    Public Health Service. Public health service policy on humane care and the use of laboratory animals. Washington, DC: US Department of Health and Human Services, 1996: 99–158.Google Scholar

  • 14.

    Lu YF, Wan XL, Xu Y, Liu J. Repeated oral administration of oleanolic acid produces cholestatic liver injury in mice. Molecules 2013;18:3060–71.Web of ScienceGoogle Scholar

  • 15.

    Nurrochmad A, Margono SA, Sardjiman, Hakim AR, Ernawati, Kurniawati E, et al. Hepatoprotective and antioxidant activity of pentagamavunon-0 against carbon tetrachloride-induced hepatic injury in rats. Asian Pac J Trop Med 2013;6:438–42.Web of ScienceGoogle Scholar

  • 16.

    Gumieniczek A. Effects of repaglinide on oxidative stress in tissues of diabetic rabbits. Diabetes Res Clin Pract 2005;68:89–95.Google Scholar

  • 17.

    Khan RA, Daud A. Protective effects of Digera muricata (L.) Mart. on testis against oxidative stress of carbon tetrachloride in rat. Food Chem Toxicol 2009;47:1393–9.Google Scholar

About the article

Corresponding author: Dr. Ademola A. Oyagbemi, Faculty of Veterinary Medicine, Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan 200005, Nigeria, Phone: +234 8033639776, Fax: +234 028103043, E-mail: ;


Received: 2013-10-10

Accepted: 2014-04-04

Published Online: 2014-05-29

Published in Print: 2015-05-01


Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, Volume 26, Issue 3, Pages 253–257, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2013-0143.

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