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Licensed Unlicensed Requires Authentication Published by De Gruyter August 7, 2014

Role for monoaminergic systems in the antidepressant and anxiolytic properties of the hydroethanolic leaf extract from Adenia cissampeloides

  • Ismail O. Ishola , Sunday O. Olayemi EMAIL logo , Omoniyi K. Yemitan and Kolawole Akinseye

Abstract

Background:Adenia cissampeloides (Planch ex. Hook) Harms (Passifloraceae) leaf infusion is used in traditional African medicine as a stimulant to treat depression and insanity. Thus, this study investigates antidepressant and anxiolytic activities of the hydroethanol leaf extract of Adenia cissampeloides (ACE) in mice.

Methods: ACE (50–200 mg/kg, p.o.) was administered to mice 1 h before behavioral studies; the forced swimming test (FST), tail suspension test (TST), elevated-plus maze test (EPM) hole-board test (HBT) and open field test (OFT). In addition, the probable mechanisms of antidepressant- and anxiolytic-like actions of ACE were also investigated.

Results: ACE (100 and 200 mg/kg) produced significant (p<0.01) reduction in immobility, along with a significant increase in swimming activity (75.20%) and climbing (190.00%), respectively, similar to anti-immobility effect of imipramine in the FST. Also, in TST, ACE (100 and 200 mg/kg) treatment significantly (p<0.01) reduced the immobility time by 35.60%, and 35.27%, respectively, which was similar to anti-immobility effect of fluoxetine (32.50%). However, the antidepressant-like effect produced by ACE was prevented (p<0.01) by yohimbine (α2-adrenoceptor antagonist), or sulpiride (dopamine D2 receptor antagonist) pretreatment. ACE (50 and 100 mg/kg) treatment (p<0.01) increased number (41.67%) and duration of head-dips (52.27%) in HBT. Similarly, ACE (50–200 mg/kg) increased duration of open arm entries (p<0.001) in EPM. However, this effect was reversed (p<0.001) by pretreatment of mice with cyproheptadine (5-HT2 receptor antagonist) (60.87%).

Conclusions: Findings from these studies revealed antidepressant-like effect of ACE mediated through interaction with dopamine D2- receptor or α2-adrenoceptor. Also an anxiolytic-like effect through interaction with 5-HT2 receptors.


Corresponding author: Sunday O. Olayemi, Faculty of Basic Medical Sciences, College of Medicine, Department of Pharmacology, Therapeutics and Toxicology, University of Lagos, PMB 12003 Surulere, Lagos, Nigeria, Mobile: +2348023150200, E-mail:

Acknowledgments

The authors appreciate Mr. C.C. Micah of the Department of Pharmacology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria for his technical assistance.

Author contributions: All authors have accepted responsibility for the entire content of the original manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

1. Kessler RC, Gruber M, Hettema JM, Hwang I, Sampson N, Yonkers KA. Co-morbid major depression and generalized anxiety disorders in the National Comorbidity Survey follow-up. Psychol Med 2008;38:365–74.10.1017/S0033291707002012Search in Google Scholar PubMed PubMed Central

2. Conway KP, Compton W, Stinson FS, Grant BF. Lifetime comorbidity of DSM-IV mood and anxiety disorders and specific drug use disorders: results from the National Epidemiologic Survey on Alcohol and Related Conditions. J Clin Psychiatry 2006;67:247–57.10.4088/JCP.v67n0211Search in Google Scholar

3. Foyet HS, Tsala DE, Bouba AA, Hritcu L. Anxiolytic and Antidepressant-like effects of the aqueous extract of Alafia multiflora Stem Barks in Rodents. Adv Pharmacol Sci 2012;2012:912041.Search in Google Scholar

4. Bandelow B, Zohar J, Hollander E, Kasper S, Möller HJ; WFSBP Task Force on Treatment Guidelines for Anxiety, Obsessive-Compulsive and Post-Traumatic Stress Disoders, et al. WFSBP Task Force on Treatment Guidelines for Anxiety, Obsessive- Compulsive and Post-Traumatic Stress Disorders World Federation of Societies of Biological Psychiatry (WFSBP) guidelines for the pharmacological treatment of anxiety, obsessive-compulsive and post-traumatic stress disorders – first revision. World J Biol Psychiatry 2008;9:248–312.10.1080/15622970802465807Search in Google Scholar PubMed

5. Inoue T, Kitaichi Y, Koyama T. SSRIs and conditioned fear. Prog Neuropsychopharmacol Biol Psychiatry 2011;35:1810–9.10.1016/j.pnpbp.2011.09.002Search in Google Scholar PubMed

6. Mora S, Dıaz-Veliz G, Millan R, Lungenstrass HS. Quiros S, Coto-Morales T, et al. Anxiolytic and antidepressant-like effects of the hydroalcoholic extract from Aloysia polystachya in rats. Pharmacol Biochem Behav 2005;82:373–8.10.1016/j.pbb.2005.09.007Search in Google Scholar PubMed

7. Zhang Z. Therapeutic effects of herbal extracts and constituents in animal models of psychiatric disorders. Life Sci 2004;75:1659–99.10.1016/j.lfs.2004.04.014Search in Google Scholar PubMed

8. Burkill HM. The useful plants of west tropical Africa, 2nd ed., Volume 4. Families M-R. Richmond, United Kingdom: Royal Botanic Gardens, Kew.Search in Google Scholar

9. Schmelzer GH, Gurib-Fakim A. Plant resources of tropical Africa 11(1) – Medicinal Plants, Netherlands: PROTA, Wageningen, 2008;791.Search in Google Scholar

10. Nyarko AA, Addy ME. Effect of aqueous extract of Adenia cissampeloides on blood pressure and serum analytes of hypertensive patients. Phytother Res 1990;4:25–8.10.1002/ptr.2650040107Search in Google Scholar

11. Annan K, Sarpong K, Asare C, Dickson R, Amponsah K, Gyan B, et al. In vitro anti-plasmodial activity of three herbal remedies for malaria in Ghana: Adenia cissampeloides (Planch.) Harms., Termina liaivorensis A. Chev, and Elaeis guineensis Jacq. Pharmacogn Res 2012;4:225–9.10.4103/0974-8490.102270Search in Google Scholar PubMed PubMed Central

12. NIH. Guide for the Use of Laboratory Animals DHHS, PHS. NIH Publication No. 85–23 (1985 Revised).Search in Google Scholar

13. Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 2005;4: 685–8.10.5897/AJB2005.000-3127Search in Google Scholar

14. The Organisation of Economic Co-operation and Development (OECD). The OECD Guideline for Testing of Chemical: 420 Acute Oral Toxicity, OECD, Paris, 2001;1–14.Search in Google Scholar

15. Ishola IO, Chatterjee M, Tota S, Narender T, Adeyemi OO, Palit G, et al. Antidepressant and anxiolytic effects of amentoflavone isolated from Cnestis ferruginea in mice. Pharmacol Biochem Behav 2012;103:322–31.10.1016/j.pbb.2012.08.017Search in Google Scholar PubMed

16. Porsolt RD, Bertin A, Jalfre M. Behavioural despair in mice: a primary screening test for antidepressants. Arch Int Pharmacodyn Ther 1977;229:327–36.Search in Google Scholar

17. Steru L, Chermat R, Thierry B, Simon P. The tail suspension test: a new method for screening antidepressants in mice. Psychopharmacol 1985;85:367–70.10.1007/BF00428203Search in Google Scholar

18. Ishola IO, Akinyede AA, Sholarin AM. Antidepressant and anxiolytic properties of the methanolic extract of momordica charantia linn (cucurbitaceae) and its mechanism of action. Drug Res (Stuttg) 2013;PMID:2422747.10.1055/s-0033-1358712Search in Google Scholar

19. Ulak G, Muttu O, Tanyen P, Komosuoghi FI, Akar FY, Erden BF. Involvement of serotonin receptor subtypes in the antidepressant-like effect of trim in the rat forced swimming test. Pharmacol Biochem Behav 2010;95:308–14.10.1016/j.pbb.2010.02.006Search in Google Scholar

20. Moreira EG, Nascimento N, Rogero JR, Vassilieff VS. Gabaergic benzodiazepine system is involved in the crotoxin-induced anxiogenic effect. Pharmacol Biochem Behav 2000;65:7–13.10.1016/S0091-3057(99)00177-XSearch in Google Scholar

21. Braida D, Capurro V, Zani A, Rubino T, Viganò D, Parolaro D. et al. Potential anxiolytic- and antidepressant-like effects of salvinorin A, the main active ingredient of Salvia divinorum, in rodents. Bri J Pharmacol 2009;157:844–53.10.1111/j.1476-5381.2009.00230.xSearch in Google Scholar

22. Lister RG. The use of a plus-maze to measure anxiety in the mouse. Psychopharmacol 1987;92:180–5.10.1007/BF00177912Search in Google Scholar

23. Ishola IO, Olayemi SO, Idowu AR. Anticonvulsant, anxiolytic and hypnotic effects of aqueous bulb extract of Crinum glaucum A. chev (Amaryllidaceae): role of GABAergic and nitrergic systems. Pak J Biol Sci 2013;16:701–10.10.3923/pjbs.2013.701.710Search in Google Scholar

24. Cryan JF, Markou A, Lucki I. Assessing antidepressant activity in rodents: recent developments and future needs. Trend Pharmacol Sci 2002;23:238–45.10.1016/S0165-6147(02)02017-5Search in Google Scholar

25. Cryan JF, Valentino RJ, Lucki I. Assessing substrates underlying the behavioral effects of antidepressants using the modified rat forced swimming test. Neurosci Biobehav Rev 2005;29:547–69.10.1016/j.neubiorev.2005.03.008Search in Google Scholar PubMed

26. Cryan JF, Mombereau C, Vassout A. The tail suspension test as a model for assessing antidepressant activity: review of pharmacological and genetic studies in mice. Neurosci Biobehav Rev 2005;29:571–625.10.1016/j.neubiorev.2005.03.009Search in Google Scholar PubMed

27. Elhwuegi AS. Central monoamines and their role in major depression. Prog Neuropsychopharmacol Biol Psychiatry 2004;28:435–51.10.1016/j.pnpbp.2003.11.018Search in Google Scholar

28. Cryan JF, Lucki I. Antidepressant-like behavioral effects mediated by 5-Hydroxytryptamine (2C) receptors. J Pharmacol Exp Ther 2000;295:1120–6.Search in Google Scholar

29. Nutt DJ. The role of dopamine and norepinephrine in depression and antidepressant treatment. J Clin Psychiatry 2006;67:3–8.Search in Google Scholar

30. Masuda Y, Ohnuma S, Sugiyama T. Alpha 2-adrenoceptor activity induces the antidepressant-like glycolipid in mouse forced swimming. Methods Find Exp Clin Pharmacol 2001;23:19–21.10.1358/mf.2001.23.1.619175Search in Google Scholar

31. Cervo L, Samanin R. Clonidine causes antidepressant-like effects in rats by activating alpha 2-adrenoceptors outside the locus coeruleus. Eur J Pharmacol 1991;193:309–13.10.1016/0014-2999(91)90144-FSearch in Google Scholar

32. Papakostas GI. Dopaminergic-based pharmacotherapies for depression. Eur Neuropsychopharmacol 2006;16:391–402.10.1016/j.euroneuro.2005.12.002Search in Google Scholar

33. Yamada J, Sugimoto Y, Yamada S. Involvement of dopamine receptors in the antiimmobility effects of dopamine re-uptake inhibitors in the forced swimming test. Eur J Pharmacol 2004;504:207–11.10.1016/j.ejphar.2004.09.057Search in Google Scholar

34. Dagytė G, Den Boer JA, Trentani A. The cholinergic system and depression. Behav Brain Res 2011;221:574–82.10.1016/j.bbr.2010.02.023Search in Google Scholar

35. Furey ML, Drevets WC. Antidepressant efficacy of the antimuscarinic drug scopolamine: a randomized, placebo-controlled clinical trial. Arch Gen Psychiatry 2006;63:1121–9.10.1001/archpsyc.63.10.1121Search in Google Scholar

36. Masse F, Petit-Demouliere B, Dubois I, Hascöet M, Bourin M. Anxiolytic-like effects of DOI microinjections into the hippocampus (but not the amygdala nor the PAG) in the mice four plates test. Behav Brain Res 2008;188:291–7.Search in Google Scholar

37. Soares VP, Zangrossi Jr, H. Stimulation of 5-HT1A or 5-HT2A receptors in the ventrolateral periaqueductal gray causes anxiolytic-, but not panicolytic-like effect in rats. Behav Brain Res 2009;197:178–85.10.1016/j.bbr.2008.08.027Search in Google Scholar

38. Brüning CA, Prigol M, Roehrs JA, Nogueira CW, Zeni G. Involvement of the serotonergic system in the anxiolytic-like effect caused by m-trifluoromethyl-diphenyl diselenide in mice. Behav Brain Res 2009;205:511–7.10.1016/j.bbr.2009.08.010Search in Google Scholar

39. Goloubkova TD, Heckler E, Rates SM, Henriques JA, Henriques AT. Inhibition of cytochrome P450-dependent monooxygenases by an alkaloid fraction from Helietta apiculata markedly potentiate the hypnotic action of pentobarbital. J Ethnopharmacol 1998;60:141–48.10.1016/S0378-8741(97)00139-6Search in Google Scholar

40. Williamson EM, Okpako DT, Evans FJ. Pharmacological methods in phytotherapy research: Selection, preparation and pharmacological evaluation of plant materials, John Wiley: Chichester 1996;184–6.Search in Google Scholar

41. Dias GP, Cavegn N, Nix A, do Nascimento-Bevilaqua MC, Stangl D, Zainuddin MS, et al. The role of dietary polyphenols on adult hippocampal neurogenesis: molecular mechanisms and behavioral effects on depression and anxiety. Oxid Med Cellular Longev 2012;541971:1–18.10.1155/2012/541971Search in Google Scholar PubMed PubMed Central

42. An L, Zhang Y, Yu N, Liu X, Zhao N, Yuan L, et al. Role for serotonin in the antidepressant-like effect of a flavonoid extract of Xiaobuxin-Tang. Pharmacol Biochem Behav 2008; 89: 572–80.10.1016/j.pbb.2008.02.014Search in Google Scholar PubMed

43. Vignes M, Maurice T, Lanté F, Nedjar M, Thethi K, Guiramand J, et al. Recasens M. Anxiolytic properties of green tea polyphenol (-)-epigallocatechin gallate (EGCG). Brain Res 2006;1110:102–15.10.1016/j.brainres.2006.06.062Search in Google Scholar PubMed

44. Zhu WL, Shi S, Wei YM, Wang SJ, Sun CY, Ding ZB, et al. Green tea polyphenols produce antidepressant-like effects in adult mice. Pharmacol Res 2012;65:74–80.10.1016/j.phrs.2011.09.007Search in Google Scholar PubMed

Received: 2014-2-17
Accepted: 2014-6-22
Published Online: 2014-8-7
Published in Print: 2015-5-1

©2015 by De Gruyter

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