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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu / Tam, Joseph

CiteScore 2016: 1.01

SCImago Journal Rank (SJR) 2016: 0.349
Source Normalized Impact per Paper (SNIP) 2016: 0.495

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Volume 26, Issue 3


Acute high blood glucose level attenuates histamine-stimulated acid secretion in male Wistar rats

Taofeek O. Usman / Lawrence A. Olatunji
  • Department of Physiology, College of Health Sciences, University of Ilorin, Ilorin, Nigeria
  • Cardiovascular Research Institute and Department of Pharmacology, Kyungpook National University School of Medicine, Daegu, Republic of Korea
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Abdul Rasak A. Alada
Published Online: 2014-12-03 | DOI: https://doi.org/10.1515/jbcpp-2014-0022


Background: Studies have shown that the hyperglycemic state induced by intravenous glucagon or glucose infusion may lead to inhibition of gastric acid secretion through the inhibition of gastric vagus activity. Histamine is a well-known mammalian acid secretagogue and it stimulates acid secretion through H2 receptors. Therefore, the aim of this study was to determine what effect an acute hyperglycemic or hypoglycemic state would have on histamine-stimulated acid secretion.

Methods: Male Wistar rats weighing between 200 and 250 g were divided into three groups that received an intravenous infusion of normal saline (control group), glucose (hyperglycemic group), or insulin (hypoglycemic group) followed by injection of histamine to stimulate acid secretion. The oesophageal and duodenal ends of the rats’ stomach were cannulated with polythene tubing and the stomach was perfused at room temperature using a Langendoff perfusion apparatus. The effluent was collected in aliquots over 10 min per sample and its pH was measured.

Results: There was a significant (p<0.05) decrease in acid secretion following histamine injection in the hyperglycemic rats, while histamine injection led to an increase (p<0.05) in acid secretion in the hypoglycemic rats.

Conclusions: These results show that hyperglycemic state would result in attenuated histamine-stimulated gastric acid secretion, while hypoglycemic state would lead to increased histamine-stimulated gastric acid secretion.

Keywords: acid secretion; histamine; hyperglycemia; hypoglycemia; insulin


  • 1.

    Henry JB. Clinical diagnosis and management by laboratory methods, 20th ed. Philadelphia: Saunders, 2001.Google Scholar

  • 2.

    Giugliano D, Marfella R, Coppola L. Vascular effects of acute hyperglycemia in humans are reversed by l-arginine. Evidence for reduced availability of nitric oxide during hyperglycemia. Circulation 1997;95:1783–90.Google Scholar

  • 3.

    Cryer PE. Hypoglycemia. In: Jefferson L, Cherrington A, Goodman H, for the American Physiological Society, editors. Handbook of physiology; section 7. The endocrine system. II. The endocrine pancreas and regulation of metabolism. New York: Oxford University Press, 2001:1057–92.Google Scholar

  • 4.

    Cryer PE, Axelrod L, Grossman AB, Heller SR, Montori VM, Seaquist ER, et al. Evaluation and management of adult hypoglycemic disorders: an Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab 2009;94:709–28.Web of ScienceGoogle Scholar

  • 5.

    Marieb E. Human anatomy and physiology. San Francisco: Benjamin Cummings, 2001:414.Google Scholar

  • 6.

    Oyebola DD, Alada AR. High doses of histamine required for gastric acid secretion in the common African toad, Bufo regularis. Isr J Med Sci 1992;28:731–3.Google Scholar

  • 7.

    Melrose AG. Effect of glucagon on gastric secretion in man. Gut 1960;1:142.Google Scholar

  • 8.

    Lam WF, Masclee AA, Muller ES, Lamers CB. Effect of hyperglycemia on gastric acid secretion and gastrin release induced by intravenous amino acids. Am J Clin Nutr 1995;61:1268–72.Google Scholar

  • 9.

    Doong ML, Yang H. Intravenous glucose infusion decreases intracisternal thyrotropin-releasing hormone induced vagal stimulation of gastric acid secretion in anesthetized rats. Neurosci Lett 2003;340:49–52.Google Scholar

  • 10.

    Loud FB, Holst JJ, Rehfeld JF, Christiansen J. Inhibition of gastric acid secretion in humans by glucagon during euglycemia, hyperglycemia and hypoglycemia. Dig Dis Sci 1988;33:530–4.Google Scholar

  • 11.

    Lam WF, Masclee AA, De Boer SY, Lamers CB. Hyperglycemia reduces gastric secretory and plasma pancreatic polypeptide responses to modified sham feeding in humans. Digestion 1993;54:48–53.CrossrefPubMedGoogle Scholar

  • 12.

    Lam WF, Masclee AA, Muller ES, Lamers CB. Effect of hyperglycemia on gastric acid secretion during gastric phase of digestion. Gastroenterology 1994;106:A119 (abstract).Google Scholar

  • 13.

    Afsaneh E, Mahdi N. Effect of d-glucose injection into ventromedial and lateral nuclei of hypothalamus on pentagastrin-induced gastric secretion in rat. Physiol Pharmacol 2002;6:67–78.Google Scholar

  • 14.

    Sakaguchi T, Aono T, Ohtake M. Enhanced gastric acid secretion induced by gastrin can be suppressed partially by glucose injection into the medullary nuclei in rats. Brain Res 1992;596:337–9.Google Scholar

  • 15.

    Simici D, Popesco M, Diculesco G. L’action de l’insuline sur la secretion de l’estomac A I’etat normal et pathologique. Arch Mal Appar Dig 1927;17:2843.Google Scholar

  • 16.

    Qvigstad G, Björgaas M, Eide I, Sandvik AK, Waldum HL. Vagal stimulation augments maximal (pental) gastrin-stimulated acid secretion in humans. Acta Physiol Scand 1999;165:277–81.Google Scholar

  • 17.

    Chang FY, Chena TS, Lee SD, Doong ML, Yeh GH, Wang PS. Pentagastrin-induced gastric acid secretion in the diabetic rats: role of insulin. Chin J Physiol 2004;47:175–81.Google Scholar

  • 18.

    Tashima K, Nishijima M, Fujita A, Kawauchi S, Takeuchi K. Gastric acid secretion in streptozotocin-diabetic rats – different responses to various secretagogues. J Physiol 2000;94:11–7.Google Scholar

  • 19.

    Herzog RI, Chan O, Yu S, Dziura J, McNay EC, Sherwin RS. Effect of acute and recurrent hypoglycemia on changes in brain glycogen concentration. Endocrinology 2008;149:1499–504.Web of ScienceGoogle Scholar

  • 20.

    Alada AR, Salahdeen HM, Akande OO, Idolor GO. Influence of nitric oxide on histamine and carbachol-induced gastric acid secretion in the common African toad – Bufo regularis. Nig J Phys Sci 2005;20:74–8.Google Scholar

  • 21.

    Amure BO, Ginsburg M. Inhibitors of Histamine catabolism and the action of gastrin in the rat. Br J Pharmacol Chemother 1964;23:476–85.CrossrefGoogle Scholar

  • 22.

    Filaretova L, Tanaka A, Miyazawa T, Kato S, Takeuchi K. Mechanisms by which endogenous glucocorticoid protects against indomethacin-induced gastric injury in rats. Am J Physiol Gastrointest Liver Physiol 2002;283:G1082–9.Google Scholar

  • 23.

    Gonlachanvit S, Chen YH, Hasller WL, Sun WL, Owyang C. Ginger reduces hyperglycemia-evoked gastric dysrhythmias in healthy humans: possible role of endogenous prostaglandins. J Pharmacol Exp Ther 2003;307:1098–103.Google Scholar

  • 24.

    Hersey SJ, Sachs G. Gastric acid secretion. Physiol Rev 1995;75:155.Google Scholar

  • 25.

    Ekelund M, Håkanson R, Hedenbro J, Liedberg G, Lundquist I, Rehfeld JF, et al. Effects of insulin on serum gastrin concentrations, gastric acid secretion and histamine mobilization in the rat. Acta Physiol Scand 2008;114:17–29.Google Scholar

About the article

Corresponding author: Taofeek O. Usman, Department of Physiology, College of Health Sciences, University of Ilorin. P.M.B. 1515 Ilorin Kwara State, Nigeria, Phone: +2348068449324, E-mail:

Received: 2014-03-05

Accepted: 2014-08-16

Published Online: 2014-12-03

Published in Print: 2015-05-01

Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, Volume 26, Issue 3, Pages 259–263, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2014-0022.

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