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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu / Tam, Joseph

CiteScore 2016: 1.01

SCImago Journal Rank (SJR) 2016: 0.349
Source Normalized Impact per Paper (SNIP) 2016: 0.495

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Volume 26, Issue 5


Hepatoprotective and antioxidant activity of hydromethanolic extract of Daniella oliveri leaves in carbon tetrachloride-induced hepatotoxicity in rats

Samuel Okwudili Onoja
  • Corresponding author
  • Department of Veterinary Physiology, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, Micheal Okpara University of Agriculture, Umudike, Umuahia, Abia State, Nigeria
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Gideon Kelechi Madubuike
  • Department of Veterinary Physiology, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, Micheal Okpara University of Agriculture, Umudike, Umuahia, Abia State, Nigeria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Maxwell Ikechukwu Ezeja
  • Department of Veterinary Physiology, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, Micheal Okpara University of Agriculture, Umudike, Umuahia, Abia State, Nigeria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-04-28 | DOI: https://doi.org/10.1515/jbcpp-2014-0087


Background: Daniella oliveri is a deciduous plant that is commonly found in savanna and open grassland. Various parts of the plant is used by herbalist in the management of different ailments. The present study aims at investigating the hepatoprotective and antioxidant activity of the methanolic extract of D. oliveri leaves.

Methods: The hepatoprotective activity was investigated using carbon tetrachloride (CCl4)-induced hepatotoxicity in albino rats. The antioxidant activity was determined using both in vitro (2, 2-diphenyl-1-picrylhydrazine photometric assay) and in vivo (malondialdehyde and catalase level assay) models.

Results: The pretreatment with extract (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) produced a significant (p<0.05) dose-dependent increase in hepatoprotective activity when compared with the negative control group. The extract (25–400 μg/mL concentration) produced a concentration-dependent increase in antioxidant activity in 2, 2-diphenyl-1-picrylhydrazine (DPPH) photometric assay. The IC50 of the extract in DPPH photometric assay was 400 μg/mL concentrations. The extract and silymarin showed a significant (p<0.05) dose-dependent increase in catalase level in treated rats when compared with the negative control group. Also, the extract and silymarin produced a significant (p<0.05) dose-dependent decrease in malondialdehyde level in treated rats when compared with the negative control group.

Conclusions: The results of the study suggest that D. oliveri leaves has a potent hepatoprotective activity that may be linked to its antioxidant activities and validates its use in the traditional management of liver disorders.

Keywords: carbon tetrachloride; catalase; Daniella oliveri; hepatoprotective activity; malondialdehyde; silymarin


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About the article

Corresponding author: Samuel Okwudili Onoja, Department of Veterinary Physiology, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, Micheal Okpara University of Agriculture, Umudike, P.M.B 7267 Umuahia, Abia State, Nigeria, Phone: +234-803-061-3032, E-mail:

Received: 2014-08-03

Accepted: 2015-03-25

Published Online: 2015-04-28

Published in Print: 2015-09-01

Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, Volume 26, Issue 5, Pages 465–470, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2014-0087.

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