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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu / Tam, Joseph

CiteScore 2016: 1.01

SCImago Journal Rank (SJR) 2016: 0.349
Source Normalized Impact per Paper (SNIP) 2016: 0.495

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Volume 26, Issue 5


Cyperus rotundus L. prevents non-steroidal anti-inflammatory drug-induced gastric mucosal damage by inhibiting oxidative stress

Deepa Thomas / Sindhu Govindhan / Edathiruthykottuckkal Chandran Baiju / Ganesan Padmavathi
  • Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ajaikumar B. Kunnumakkara
  • Corresponding author
  • Amala Cancer Research Center, Amala Nagar, Thrissur, Kerala, India
  • Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, India
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jose Padikkala
Published Online: 2015-04-25 | DOI: https://doi.org/10.1515/jbcpp-2014-0093


Background: Since centuries, Cyperus rotundus L. has been used against gastric ailments in traditional Indian medicine, especially in Ayurveda and Siddha. Therefore, it is very obvious that this plant will have a greater potential to treat gastric ulcers. For this reason, in this study, we mainly focused on the ulcer-preventive role of C. rotundus in rats treated with non-steroidal anti-inflammatory drugs.

Methods: Seventy percent methanolic extract of the plant was prepared and fed to 36-h fasted rats. Ulcer was induced in these rats by single oral administration of aspirin (400 mg/kg) 1 h after the administration of the plant extract. After 4 h, the rats were sacrificed, ulcer index was calculated, and antioxidant activity of the extract in gastric mucosa was evaluated by determining the levels of superoxide dismutase, glutathione, glutathione peroxidase, and tissue lipid peroxidation.

Results: Oral administration of different doses of C. rotundus rhizome methanolic extract (CME; 250 mg/kg and 500 mg/kg) significantly inhibited aspirin-induced gastric ulceration in animals in a dose-dependent manner (49.32% and 53.15%, respectively), which was also comparable with the standard gastric ulcer drug ranitidine. Administration of CME also significantly increased the activity of superoxide dismutase, cellular glutathione and glutathione peroxidase, and inhibited the lipid peroxidation in the gastric mucosa of ulcerated animals in a dose-dependent manner.

Conclusions: Our results showed that C. rotundus extract has the capacity to significantly inhibit aspirin-induced gastric ulcers through an antioxidant defense mechanism. This study warrants further examination of this plant for its gastroprotective activities.

Keywords: aspirin; Cyperus rotundus; oxidative damage; ranitidine; ulcer


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About the article

Corresponding authors: Dr. Ajaikumar B. Kunnumakkara, PhD, Associate Professor, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Room # 005, O-Block, Guwahati, Assam 781039, India, Phone: +91-361-258-2231 (office), +91-789-600-5326 (mobile), Fax: +91-361-258-2249 (office), E-mail: ; ; and Amala Cancer Research Center, Amala Nagar, Thrissur, Kerala, India; and Jose Padikkala, PhD, Professor and Head Department of Biotechnology, Associate Director, Amala Cancer Research Center, Amala Nagar, Kerala, India, Phone: +91-0487-2307968, Fax: +91-0487-2307968, E-mail:

Received: 2014-08-09

Accepted: 2015-02-19

Published Online: 2015-04-25

Published in Print: 2015-09-01

Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, Volume 26, Issue 5, Pages 485–490, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2014-0093.

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