Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu / Tam, Joseph


CiteScore 2016: 1.01

SCImago Journal Rank (SJR) 2016: 0.349
Source Normalized Impact per Paper (SNIP) 2016: 0.495

Online
ISSN
2191-0286
See all formats and pricing
More options …
Volume 27, Issue 1

Issues

Anti-diabetic effects of aqueous prickly lettuce (Lactuca scariola Linn.) leaves extract in alloxan-induced male diabetic rats treated with nickel (II)

Kailash S. Chadchan
  • Department of Chemistry, B.L.D.E.A’s V.P. Dr. P.G. Halakatti College of Engineering and Technology, Bijapur, Karnataka, India, (Affiliated to Visvesvaraya Technological University, Belagavi, Karnataka, India)
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jameel G. Jargar / Swastika N. Das
  • Corresponding author
  • Department of Chemistry, B.L.D.E.A’s V.P. Dr. P.G. Halakatti College of Engineering and Technology, Bijapur, Karnataka, India, (Affiliated to Visvesvaraya Technological University, Belagavi, Karnataka, India)
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-08-26 | DOI: https://doi.org/10.1515/jbcpp-2015-0038

Abstract

Background: Hattaraki pallye or prickly lettuce (Lactuca scariola Linn.) is one among several green leafy plants that grow in north Karnataka; it is usually consumed by the people of this region and is found to be antidiabetic in nature. The objective of this study is to evaluate hypoglycemic activities of supplementation with aqueous extract of prickly lettuce (L. scariola) leaves in vivo in acute and subchronic exposure with or without nickel (II) along with its glucose reduction capabilities with or without nickel (II) at pH 7.0 and 9.0 in vitro.

Methods: Percentage glucose reduction (in vitro) was determined by glucose oxidase-peroxidase enzymatic method at pH 7.0 and pH 9.0 using UV-Vis spectrophotometer. Hypoglycemic activities of L. scariola were carried out in alloxan-induced male diabetic rats at both acute and subchronic exposure.

Results: The results showed a significant alteration in the λmax value of Ni (II) in combination with L. scariola leaves extracts at both pH 7.0 and 9.0. The aqueous extract also produced a significant reduction in the glucose concentration at pH 7.0 and pH 9.0 even in presence of Ni (II) in vitro. Lactuca scariola leaves in either acute or subchronic supplementation showed a greater glucose tolerance and hypoglycemic regulation of blood sugar in diabetic rats with or without nickel (II) treatments.

Conclusions: Lactuca scariola leaves can be a substitute for synthetic drugs to treat diabetic patients.

Keywords: hypoglycemia; Lactuca scariola Linn. extract; nickel (II); pH alteration

References

  • 1.

    Ody P. The complete medicinal herbal, 1st ed. New York: Dorling Kindersley publications, Inc., 1993.Google Scholar

  • 2.

    Rajasab AH, Isaq M. Documentation of folk knowledge on edible wild plants of North Karnataka. Indian J Trad Know 2004;3:419–29.Google Scholar

  • 3.

    Chen YW, Yang CY, Huang CF, Hung DZ, Leung YM, Liu SH. Heavy metals, islet function and diabetes development. Islets 2009;1:169–76.Web of ScienceGoogle Scholar

  • 4.

    Grover JK, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmocol 2002;81:81–100.CrossrefGoogle Scholar

  • 5.

    Gamble JS. The flora of the presidency of Bombay. Vol. III. Taylor and Francis publication, London, 1908.Google Scholar

  • 6.

    Khandelwal KR. Practical pharcognosy techniques and experiments, 11th ed. Pune: Nirali Prakashan, 2004:149–56.Google Scholar

  • 7.

    Malik CP, Singh MB, editors. Plant enzymology and histoenzymology. New Delhi: Kalyani Publishers, 1980:278.Google Scholar

  • 8.

    Das KK, Razzaghi-Asl N, Tikare SN, Di Santo R, Costi R, Messore A, et al. Hypoglycemic activity of curcumin synthetic analogues in alloxan-induced diabetic rats. J Enzyme Inhib Med Chem 2015:1–7. [Epub ahead of print]. DOI: 10.3109/14756366.2015.1004061.CrossrefGoogle Scholar

  • 9.

    OECD 2001. Guidelines for the testing of chemicals, revised draft guidelines 423, Acute oral toxicity – acute toxic class method, revised document, CPCSEA, Ministry of Social Justice and Empowerment. New Delhi: Government of India.Google Scholar

  • 10.

    Bordes E, Papillion VV. Myocardial change induced by nickel and in association with cadmium. Rev Ig Bacteriol Virusal Parazitol Epidemol Pneumotizol 1983;32:51–6.Google Scholar

  • 11.

    El-Hilaly J, Tahraoui A, Israili ZH, Lyoussi B. Hypolipidemic effects of acute and sub-chronic administration of an aqueous extract of Ajugaiva L. whole plant in normal and diabetic rats. J Ethnopharmacol 2006;105:441–8.CrossrefGoogle Scholar

  • 12.

    Dhandapani S, Ramasamy SV, Rajagopal S, Namasivayam, N. Hypolipidemic effect of Cuminumcyminum L. on alloxan-induced diabetic rats. Pharmacol Res 2002;46:251–5.CrossrefGoogle Scholar

  • 13.

    Trinder P. Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. J Clin Pathol 1969;22:158–61.CrossrefGoogle Scholar

  • 14.

    Sridevi S, Chary MG, Krishna DR, Diwan PV. Pharmacodynamic evaluation of transdermal drug delivery system of glibenclamide in rats. Indian J Pharmacol 2000;32:309–12.Google Scholar

  • 15.

    Chadchan KS, Das SN, Das KK. Fenugreek (Trigonella foenum graecum) leaves extract and its interaction with heavy metal (nickel II) with reference to glucose reduction capabilities in-vitro. Biomedicine 2014;34:104–8.Google Scholar

  • 16.

    Babu PV, Liu D, Gilbert ER. Recent advances in understanding the anti-diabetic actions of dietery flavonoids. J Nutr.Biochem 2013;24:777–89.Web of ScienceCrossrefGoogle Scholar

  • 17.

    Negi JS, Negi PS, Pant GJ, Rawat MS, Negi SK. Naturally occurring saponins: chemistry and biology. J Poisonous Med Plant Res 2013;1:6–11.Google Scholar

  • 18.

    Horak E, Zygowicz ER, Tarabishy R, Mitchell JM, Sunderman FW. Effects of nickel chloride and nickel carbonyl upon glucose metabolism in rats. Ann Clin Lab Sci 1978;8:476–82.Google Scholar

  • 19.

    Jargar JG, Dhundasi SA, Das KK. Influence of α-tocopherol on blood glucose regulation of alloxan induced male diabetic rats exposed to nickel sulfate. Biomedicine 2014; 34:296–303.Google Scholar

  • 20.

    Cartana J, Arola L. Nickel-induced hyperglycaemia: the role of insulin and glucagon. Toxicology1992;71:181–92.Google Scholar

  • 21.

    Gupta S, Ahmad N, Husain MM, Srivastava RC. Involvement of nitric oxide in nickel-induced hyperglycemia in rats. Nitric Oxide 2000;4:129–38.CrossrefGoogle Scholar

About the article

Corresponding author: Prof. Swastika N. Das, Department of Chemistry, B.L.D.E.A’s V.P. Dr. P.G. Halakatti College of Engineering and Technology, Bijapur-586103, Karnataka, India, (Affiliated to Visvesvaraya Technological University, Belagavi, Karnataka, India), Phone: +91 8553286112, E-mail:


Received: 2015-04-07

Accepted: 2015-06-16

Published Online: 2015-08-26

Published in Print: 2016-01-01


Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, Volume 27, Issue 1, Pages 49–56, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2015-0038.

Export Citation

©2016 by De Gruyter.Get Permission

Comments (0)

Please log in or register to comment.
Log in