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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu / Tam, Joseph


CiteScore 2016: 1.01

SCImago Journal Rank (SJR) 2016: 0.349
Source Normalized Impact per Paper (SNIP) 2016: 0.495

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2191-0286
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Volume 28, Issue 1

Issues

Methanol extract of Nymphaea lotus ameliorates carbon tetrachloride-induced chronic liver injury in rats via inhibition of oxidative stress

Ifeoluwa T. Oyeyemi
  • Cell Biology and Genetics Unit, Faculty of Science, Department of Zoology, University of Ibadan, Ibadan, Nigeria
  • Other articles by this author:
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/ Olubukola O. Akanni
  • Drug Metabolism and Toxicology Unit, Faculty of Basic Medical Sciences, Department of Biochemistry, University of Ibadan, Ibadan, Nigeria
  • Other articles by this author:
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/ Oluwatosin A. Adaramoye
  • Drug Metabolism and Toxicology Unit, Faculty of Basic Medical Sciences, Department of Biochemistry, University of Ibadan, Ibadan, Nigeria
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/ Adekunle A. Bakare
  • Corresponding author
  • Cell Biology and Genetics Unit, Faculty of Science, Department of Zoology, University of Ibadan, Ibadan, Nigeria
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  • Other articles by this author:
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Published Online: 2016-11-08 | DOI: https://doi.org/10.1515/jbcpp-2016-0029

Abstract

Background:

Nymphaea lotus (NL) is an aquatic perennial plant used traditionally in the management of various liver diseases. In this study, the protective effect of methanol extract of NL against carbon tetrachloride (CCl4)-induced chronic hepatotoxicity in rats was investigated.

Methods:

Male Wistar rats were assigned into six groups of five rats each. Group I received corn oil (0.5 mL p.o.) and served as control, group II received CCl4 (1 mL/kg i.p., 1:3 in corn oil), group III received NL (200 mg/kg), and groups IV, V, and VI received CCl4+NL (50, 100, and 200 mg/kg, respectively) for 6 weeks. Twenty-four hours after the last exposure, rats were bled and killed.

Results:

The activities of alanine aminotransaminase (ALT), aspartate aminotransferase (AST), and levels of total bilirubin (TB) in the serum, thiobarbituric acid reactive substances (TBARS), superoxide dismutase, catalase, glutathione peroxidase (GPx) and glutathione (GSH) in the liver, and histopathology of the liver were determined using standard procedures. NL significantly (p<0.05) lowered the levels of ALT, AST, and TB and exhibited antioxidant potentials in rats exposed to CCl4 relative to the control values. Specifically, NL at 100 and 200 mg/kg significantly (p<0.05) increased CCl4-induced decrease in hepatic GSH and GPx and also decreased the level of hepatic TBARS in CCl4-intoxicated rats. Histopathological findings revealed cellular infiltration and fibrosis in rats that received CCl4 only, which were ameliorated in rats that received NL+CCl4.

Conclusions:

The data suggest that NL exhibited hepatoprotective effects in CCl4-intoxicated rats via antioxidative mechanism.

Keywords: antioxidant enzymes; hepatotoxicity; liver fibrosis; liver histopathology; Nymphaea lotus

References

  • 1.

    Malhi H, Gores G, Lemasters J. Apoptosis and necrosis in the liver: a tale of two deaths? Hepatology 2006;43:S31–44.Google Scholar

  • 2.

    Shim JY, Kim MH, Kim HD, Ahn JY, Yun YS, Song JY. Protective action of the immunomodulator ginsan against carbon tetrachloride-induced liver injury via control of oxidative stress and the inflammatory response. Toxicol Appl Pharmacol 2010;242:318–25.Google Scholar

  • 3.

    Lee KJ, Woo ER, Choi CY, Shin DW, Lee DG, You HJ, et al. Protective effect of acteoside on carbon tetrachloride-induced hepatotoxicity. Life Sci 2004;74:1051–64.Google Scholar

  • 4.

    Toori MA, Joodi B, Sadeghi H, Sadeghi H, Jafari M, Talebianpoor S, et al. Hepatoprotective activity of aerial parts of Otostegia persica against carbon tetrachloride-induced liver damage in rats. Avi J Phytomed 2015;5:238–46.Google Scholar

  • 5.

    Hassan A, Rahman S, Deeba F, Mahmud S. Antimicrobial activity of some plant extracts having hepatoprotective effects. J Med Plants Res 2009;3:20–3.Google Scholar

  • 6.

    Akinjogunla OJ, Yah CS, Eghafona NO, Ogbemudia FO. Antibacterial activity of leave extracts of Nymphaea lotus (Nymphaeaceae) on methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Staphylococcus aureus (VRSA) isolated from clinical samples. Ann Biol Res 2010;1.2:174–84.Google Scholar

  • 7.

    Chaurasia S, Sharma V, Iqbal dar A, Arya N, Saxena RC, Chaurasia ID, et al. In vivo antidiabetic activity of alcoholic and aqueous extract of Nymphaea lotus in rat model. Inventi Rapid Ethnopharmacol 2011, Inventi:pep/496/11.Google Scholar

  • 8.

    Saleem A, Ahotupa M, Pihlaja K. Total phenolics concentration and antioxidant properties of extracts of medicinal plants of Pakistan. Z Naturforsch C 2001;56:973–8.Google Scholar

  • 9.

    Afolayan AJ, Sharaibi OJ, Kazeem MI. Phytochemical analysis and in vitro antioxidant activity of Nymphea lotus L. Int J Pharmacol 2013;9:297–304.Google Scholar

  • 10.

    Oyeyemi IT, Yekeen OM, Odusina PO, Ologun TM, Ogbaide OM, Olaleye OI, et al. Genotoxicity and antigenotoxicity of aqueous and hydro-methanol extracts of Spondias mombin (L), Nymphea lotus (L) and Luffa cylindrica (L) using animal bioassays. Interdiscip Toxicol 2015;8:101–9.Google Scholar

  • 11.

    Ashidi JS, Houghton PJ, Hylands PJ, Efferth T. Ethnobotanical survey and cytotoxicity testing of plants of south-western Nigeria used to treat cancer, with isolation of cytotoxic constituents from Cajanus cajan Millsp. Leaves. J Ethnopharmacol 2010;128:501–12.Google Scholar

  • 12.

    Hou YL, Tsai YH, Lin YH, Chao JC. Ginseng extract and ginsenoside Rb1 attenuate carbon tetrachloride-induced liver fibrosis in rats. BMC Complement Altern Med 2014;14:415–25.Google Scholar

  • 13.

    Boll M, Weber LW, Becker E, Stampfl A. Mechanism of carbon tetrachloride-induced hepatotoxicity. Hepatocellular damage by reactive carbon tetrachloride metabolites. Z Naturforsch 2001;56c:649–59.Google Scholar

  • 14.

    Cheeseman KH, Albano EF, Tomasi A, Slater TF. Biochemical studies on the metabolic activation of halogenated alkanes. Environ Health Perspect 1985;64:85–101.Google Scholar

  • 15.

    Manibusan MK, Odin M, Eastmond DA. Postulated carbon tetrachloride mode of action: a review. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 2007;25:185–209.Google Scholar

  • 16.

    Fujii T, Fuchs BC, Yamada S, Lauwers GY, Kulu Y, Goodwin JM, et al. Mouse model of carbon tetrachloride induced liver fibrosis: histopathological changes and expression of CD133 and epidermal growth factor. BMC Gastroenterol 2010;10:79.Google Scholar

  • 17.

    Lee GP, Jeong WI, Jeong DH, Do SH, Kim TH, Jeong KS. Diagnostic evaluation of carbon tetrachloride induced rat hepatic cirrhosis model. Anticancer Res 2005;25:1029–38.Google Scholar

  • 18.

    Al-Sayed E, Martiskainen O, Seif el-Din SH, Sabra AA, Hammam OA, El-Lakkany NM, et al. Hepatoprotective and antioxidant effect of Bauhinia hookeri extract against carbon tetrachloride-induced hepatotoxicity in mice and characterization of its bioactive compounds by HPLC-PDA-ESI-MS/MS. Biomed Res Int 2014, Article ID 245171.Google Scholar

  • 19.

    Gornal AG, Bardwil GS, David MM. Determination of serum proteins by mean of Biuret reactions. Biochemistry 1949;177:751–66.Google Scholar

  • 20.

    Mistra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972;247:3170–5.Google Scholar

  • 21.

    Claiborne A. Catalase activity. In: Wald RA, editor. CRC Handbook of methods for oxygen radical research. Boca Raton, FL: CRC Press, 1985:283–4.Google Scholar

  • 22.

    Beutler E, Duron O, Kelly BM. Improved method for determination of blood glutathione. J Lab Clin Med 1963;61:882–90.Google Scholar

  • 23.

    Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: biochemical role as a component of glutathione peroxidase. Science 1973;179:588–90.Google Scholar

  • 24.

    Rice-Evans C, Omorphos CS, Baysal E. Sickle cell membrane and oxidative damage. Biochem J 1986;237:265–9.Google Scholar

  • 25.

    Kalender S, Ogutcu A, Uzunhisarcikli M, Acikgoz F, Durak D, Ulusoy Y, et al. Diazinon-induced hepatotoxicity and protective effect of vitamin E on some biochemical indices and ultrastructural changes. Toxicology 2005;211:197–206.Google Scholar

  • 26.

    Edfawy M, Hassan MH, Mansour A, Hamed AA, Amin HA. Meloxicam modulates oxidative stress status, inhibits prostaglandin E2, and abrogates apoptosis in carbon tetrachloride induced rat hepatic injury. Int J Toxicol 2012;31:276–86.Google Scholar

  • 27.

    Raza M, Al-Shabanah OA, El-Hadiyah TM, Al-Majeed AA. Effect of prolonged vigabatrin treatment on heamatological and biochemical parameters in plasma, liver and kidney of Swiss albino mice. Sci Pharm 2002;70:135–45.Google Scholar

  • 28.

    Liju VB, Jeena K, Kuttan R. Acute and subchronic toxicity as well as mutagenic evaluation of essential oil from turmeric (Curcuma longa L). Food Chem Toxicol 2013;53:52–61.Google Scholar

  • 29.

    Breikaa RM, Algandaby MM, El-Demerdash E, Abdel-Naim AB. Multimechanistic antifibrotic effect of biochanin A in rats: implications of proinflammatory and profibrogenic mediators. PLoS One 2013;8:e69276.Google Scholar

  • 30.

    Vladimir-Knežević S, Cvijanović O, Blažeković B, Kindl M, Štefan MB, Domitrović R. Hepatoprotective effects of Micromeria croatica ethanolic extract against CCl4–induced liver injury in mice. BMC Complement Altern Med 2015;15:233–44.Google Scholar

  • 31.

    Uzma N, Kumar BS, Anees S. Red wine ameliorates CCl4-induced acute liver injury in rats. Austr J Biomed Sci 2011;1:1–7.Google Scholar

  • 32.

    Bhandarkar MR, Khan A. Antihepatotoxic effect of Nymphaea stellata willd., against carbon tetrachloride-induced hepatic damage in albino rats. J Ethnopharmacol 2004;91:61–4.Google Scholar

  • 33.

    Paharia AK, Pandurangan A. Evaluation of hepatoprotective activity of ethanolic extract of Nymphaea alba Linn flower in experimental rats. Int J Biomed Res 2013;4:349–54.Google Scholar

  • 34.

    Faubion WA, Guicciardi ME, Miyoshi H, Bronk SF, Roberts PJ, Svingen PA, et al. Toxic bile salts induce rodent hepatocyte apoptosis via direct activation of Fas. J Clin Invest 1999;103:137–45.Google Scholar

  • 35.

    Webster CR, Anwer MS. Cyclic adenosine monophosphate-mediated protection against bile acid-induced apoptosis in cultured rat hepatocytes. Hepatology 1998;27:1324–31.Google Scholar

  • 36.

    Fallatah HI. Noninvasive biomarkers of liver fibrosis: an overview. Adv Hepatol 2004;39:1563–73.Google Scholar

  • 37.

    Tamura H, Matsuda A, Kidoguchi N, Matsumura O, Mitarai T, Isoda K. A family with two sisters with collagenofibrotic glomerulonephropathy. Am J Kidney Dis 1996;27:588–95.Google Scholar

  • 38.

    Lieber CS, Weiss DG, Paronetto F. Value of fibrosis markers for staging liver fibrosis in patients with precirrhotic alcoholic liver disease. Alcohol Clin Exp Res 2008;32:1031–9.Google Scholar

  • 39.

    Cengiza M, Yılmazb G, Ozenirlera S. The association between indirect bilirubin levels and liver fibrosis due to chronic hepatitis C virus infection. Pathol Res Pract 2014;210:488–93.Google Scholar

  • 40.

    Singh N, Kamath V, Narasimhamurthy K, Rajini PS. Protective effects of potato peel extract against carbon tetrachloride-induced liver injury in rats. Environ Toxicol Pharmacol 2008;26:241–6.Google Scholar

  • 41.

    Wang T, Sun NL, Zhang WD, Li HL, Lu GC, Yuan BJ, et al. Protective effect of dehydrocavidine on carbon tetrachloride-induced acute hepatotoxicity in rats. J Ethnopharmacol 2008;117:300–8.Google Scholar

  • 42.

    Ai G, Liu Q, Hua W, Huang Z, Wang D. Hepatoprotective evaluation of the total flavonoids extracted from flowers of Abelmoschus manihot (L.) Medic: in vitro and in vivo studies. J Ethnopharmacol 2013;146:794–802.Google Scholar

  • 43.

    Bahashwan S, Hassan MH, Aly H, Ghobara MM, El-Beshbishy HA, Busati I. Crocin mitigates carbon tetrachloride-induced liver toxicity in rats. J Taibah Univ Med Sci 2015;10:140–9.Google Scholar

  • 44.

    Jadhav SH, Sarkar SN, Aggarwal M, Tripathi HC. Induction of oxidative stress in erythrocytes of male rats subchronically exposed to a mixture of eight metals found as groundwater contaminants in different parts of India. Arch Environ Contam Toxicol 2007;52:145–51.Google Scholar

  • 45.

    Ferrari RS, da Rosa DP, Forgiarini LF, Bona S, Dias AS, Marroni NP. Oxidative stress and pulmonary changes in experimental liver cirrhosis. Oxid Med Cell Longev 2012;2012:486190.Google Scholar

  • 46.

    Oyeyemi IT, Bakare AA. Genotoxic and anti-genotoxic effect of aqueous extracts of Spondias mombin L., Nymphea Lotus L. and Luffa cylindrica L. on Allium cepa root cells. Carylogia 2013;66:360–7.Google Scholar

  • 47.

    Al-Olayan EM, El-Khadragy MF, Aref AM, Othman MS, Kassab RB, Abdel Moneim AE. The potential protective effect of Physalis peruviana L. against carbon tetrachloride-induced hepatotoxicity in rats is mediated by suppression of oxidative stress and down regulation of MMP-9 expression. Oxid Med Cell Longev 2014:Article ID 381413.Google Scholar

  • 48.

    Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: how are they linked? Free Radic Biol Med 2010;49:1603–16.Google Scholar

  • 49.

    Parola M, Robino G. Oxidative stress-related molecules and liver fibrosis. J Hepatol 2001;35:297–306.Google Scholar

About the article

Corresponding author: Prof. Adekunle A. Bakare, Cell Biology and Genetics Unit, Faculty of Science, Department of Zoology, University of Ibadan, Ibadan, Nigeria, Phone: +234-7032295419, E-mail:


Received: 2016-03-04

Accepted: 2016-08-05

Published Online: 2016-11-08

Published in Print: 2017-01-01


Author contributions: ITO, OAA, and AAB designed the work, ITO and OOA carried out the laboratory analysis and wrote the manuscript, ITO did the statistical analysis, and OAA and AAB proofread, corrected, and approved the manuscript. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: This study was partly funded by the Alexander Von Humboldt Return Fellowship to AAB and the University of Ibadan Postgraduate School scholarship to ITO.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organizations played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, Volume 28, Issue 1, Pages 43–50, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2016-0029.

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