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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu / Tam, Joseph


CiteScore 2016: 1.01

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Volume 29, Issue 3

Issues

Effects of dizocilpine-induced glutamatergic blockade in the nucleus accumbens septi on the plus maze test

Augusto P.I. Gargiulo
  • Corresponding author
  • Laboratory of Neurosciences and Experimental Psychology, Department of Pathology, Faculty of Medical Sciences, National University of Cuyo, CONICET, 5500 Mendoza, Argentina, Phone: +54 261 4135000/5006. Int. 2644 (U. N. de Cuyo), Fax: +54 261 5244001 (CCT, Mendoza)
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/ María P. Gargiulo De Aranda
  • Laboratory of Neurosciences and Experimental Psychology, Department of Pathology, Faculty of Medical Sciences, National University of Cuyo, CONICET, 5500 Mendoza, Argentina
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/ Mercedes M.L. Gargiulo
  • Laboratory of Neurosciences and Experimental Psychology, Department of Pathology, Faculty of Medical Sciences, National University of Cuyo, CONICET, 5500 Mendoza, Argentina
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/ Angel J.M. Gargiulo
  • Laboratory of Neurosciences and Experimental Psychology, Department of Pathology, Faculty of Medical Sciences, National University of Cuyo, CONICET, 5500 Mendoza, Argentina
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/ Andres Acuña
  • Laboratory of Neurosciences and Experimental Psychology, Department of Pathology, Faculty of Medical Sciences, National University of Cuyo, CONICET, 5500 Mendoza, Argentina
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/ Gustavo C. Baiardi
  • Laboratory of Neuropharmacology, Institute of Biological and Technological Research (IIBYT-CONICET), National University of Córdoba, Córdoba, Argentina
  • Faculty of Chemical Sciences, Catholic University of Córdoba, 5017 Córdoba, Argentina
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/ José V. Lafuente
  • Laboratory of Clinical and Experimental Neurosciences, Department of Neurosciences, Faculty of Medicine and Odontology, University of the Basque Country, Bilbao, Spain
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/ Adriana I. Landa De Gargiulo
  • Laboratory of Neurosciences and Experimental Psychology, Department of Pathology, Faculty of Medical Sciences, National University of Cuyo, CONICET, 5500 Mendoza, Argentina
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/ Manuel A. Guevara
  • Laboratory of Neurosciences and Experimental Psychology, Department of Pathology, Faculty of Medical Sciences, National University of Cuyo, CONICET, 5500 Mendoza, Argentina
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/ Pascual A. Gargiulo
  • Laboratory of Neurosciences and Experimental Psychology, Department of Pathology, Faculty of Medical Sciences, National University of Cuyo, CONICET, 5500 Mendoza, Argentina
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Published Online: 2018-02-14 | DOI: https://doi.org/10.1515/jbcpp-2017-0100

Abstract

Background:

In previous studies, we have observed that specific N-methyl-d-aspartic acid (NMDA) antagonists and non-NMDA antagonists injected within the nucleus accumbens septi (NAS) induced an anxiolytic-like effect in the plus maze test in rats. In the present study, the effect of intracanalicular blockade of NMDA receptors using dizocilpine in the plus maze was studied in male rats bilaterally cannulated NAS.

Methods:

Rats were divided into five groups that received either 1 μL injections of saline or dizocilpine (MK-801, [5R,10S]-[+]-5-methyl-10,11-dihydro-5H-dibenzo [a,d] cyclohepten-5,10-imine) in different doses (0.5, 1, 2, or 4 μg) 15 min before testing.

Results:

Time spent in the open arm increased under dizocilpine treatment with the two higher doses (2 and 4 μg, p<0.05), extreme arrivals were increased by the three higher doses (1 μg, p<0.05; 2 and 4 μg, p<0.01), and open arm entries by the three higher doses (1, 2, and 4 μg, p<0.05). A dose-effect relationship was observed in all cases.

Conclusions:

We conclude that dizocilpine-glutamatergic blockade in the accumbens lead to an anxiolytic-like effect and a behavioral disinhibition related to an increase in some motoric parameters, showing specific behavioral patterns.

Keywords: accumbens; anxiety; dizocilpine; plus maze; rat

References

  • 1.

    Pennartz CM, Groenewegen HJ, Lopes da Silva F. The nucleus accumbens as a complex of functionally distinct neuronal ensembles: an integration of behavioural, electrophysiological and anatomical data. Prog Neurobiol 1994;42:719–61.PubMedCrossrefGoogle Scholar

  • 2.

    Cabib S, Puglisi-Allegra S. Stress, depression and the mesolimbic dopamine system. Psychopharmacology 1996;128:331–42.PubMedCrossrefGoogle Scholar

  • 3.

    Gargiulo PA, Landa de Gargiulo AI. Perception and psychoses: the role of glutamatergic transmission within the nucleus accumbens septi. Behav Brain Sci 2004;27:792–3.Google Scholar

  • 4.

    Gargiulo PA, Landa de Gargiulo AI. Glutamate and modeling of schizophrenia symptoms: review of our findings: 1990–2014. Pharmacol Rep 2014;66:343–52.PubMedCrossrefWeb of ScienceGoogle Scholar

  • 5.

    Matthysse S. Nucleus accumbens and schizophrenia. In: Chronister R, De France JF, editors. The neurobiology of the nucleus accumbens. Maine: Haer Institute, 1981:351–9.Google Scholar

  • 6.

    Gargiulo PA. Thyrotropin releasing hormone injected into the nucleus accumbens septi selectively increases face grooming in rats. Braz J Med Biol Res 1996;29:805–10.PubMedGoogle Scholar

  • 7.

    Koob GF. Neural mechanisms of drug reinforcement. Ann N Y Acad Sci 1992;654:171–91.CrossrefPubMedGoogle Scholar

  • 8.

    Grace AA. Gating of information flow within the limbic system and the pathophysiology of schizophrenia. Brain Res Brain Res Rev 2000;1:330–41.Google Scholar

  • 9.

    Martínez G, Ropero C, Funes A, Flores E, Blotta C, Landa AI, et al. Effects of selective NMDA and non-NMDA blockade in the nucleus accumbens on the plus-maze test. Physiol Behav 2002;76:219–24.CrossrefPubMedGoogle Scholar

  • 10.

    Martínez G, Ropero C, Funes A, Flores E, Landa, AI, Gargiulo PA. AP-7 into the nucleus accumbens disrupts acquisition but does not affect consolidation in a passive avoidance task. Physiol Behav 2002;76:205–12.CrossrefGoogle Scholar

  • 11.

    Llano López LH, Caif F, García S, Fraile M, Landa AI, Baiardi G, et al. Anxiolytic-like effect of losartan injected into amygdala of the acutely stressed rats. Pharmacol Rep 2012;64:54–63.CrossrefPubMedGoogle Scholar

  • 12.

    Llano López LH, Caif F, Fraile M, Tinnirello B, Landa de Gargiulo AI, Lafuente JV, et al. Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi. Pharmacol Rep 2013;65:566–78.CrossrefPubMedWeb of ScienceGoogle Scholar

  • 13.

    Gargiulo PA, Siemann M, Delius J. Visual discrimination in pigeons impaired by glutamatergic blockade of nucleus accumbens. Physiol Behav 1998;63:705–9.PubMedCrossrefGoogle Scholar

  • 14.

    Gargiulo PA, Acerbo MJ, Krug I, Delius JD. Cognitive effects of dopaminergic and glutamatergic blockade in nucleus accumbens in pigeons. Pharmacol Biochem Behav 2005;81:732–9.CrossrefPubMedGoogle Scholar

  • 15.

    Acerbo MJ, Gargiulo PA, Krug I, Delius JD. Behavioural consequences of nucleus accumbens dopaminergic stimulation and glutamatergic blocking in pigeons. Behav Brain Res 2002;136:171–7.PubMedCrossrefGoogle Scholar

  • 16.

    Jessa M, Nazar M, Plaznik A. Effect of intra-accumbens blockade of NMDA receptors in two models of anxiety, in rats. Neurosci Res Commun 1996;19:19–25.CrossrefGoogle Scholar

  • 17.

    Belozertseva IV, Bespalov AY. Effects of NMDA receptor channel blockers, dizocilpine and memantine, on the development of opiate analgesic tolerance induced by repeated morphine exposures or social defeats in mice. Naunyn Schmiedebergs Arch Pharmacol 1998;358:270–4.PubMedCrossrefGoogle Scholar

  • 18.

    Dimpfel W, Spüler M. Dizocilpine (MK-801), ketamine and phencyclidine: low doses affect brain field potentials in the freely moving rat in the same way as activation of dopaminergic transmission. Psychopharmacology (Berl) 1990;101:317–23.CrossrefGoogle Scholar

  • 19.

    Sircar R, Rappaport M, Nichtenhauser R, Zukin SR. The novel anticonvulsant MK-801: a potent and specific ligand of the brain phencyclidine/sigma-receptor. Brain Res 1987;435:235–40.PubMedCrossrefGoogle Scholar

  • 20.

    Pellegrino LJ, Pellegrino AS, Cushman AJ. A stereotaxic atlas of the rat brain. New York: Plenum Press, 1979.Google Scholar

  • 21.

    Gargiulo PA, Donoso AO. Distinct grooming patterns induced by intracerebroventricular injection of CRH, TRH and LHRH in male rats. Braz J Med Biol Res 1996;29:375–9.PubMedGoogle Scholar

  • 22.

    Silva RC, Brandao ML. Acute and chronic effects of gepirone and fluoxetine in rats tested in the elevated plus maze: an ethological analysis. Pharmacol Biochem Behav 2000;65:209–16.PubMedCrossrefGoogle Scholar

  • 23.

    Laconi M, Casteller G, Gargiulo PA, Bregonzio C, Cabrera R. The anxiolytic effect of allopregnenolone is associated with gonadal hormonal status in female rats. Eur J Pharmacol 2001;417:111–6.CrossrefGoogle Scholar

  • 24.

    File SE. Behavioural detection of anxiolytic action. In: Elliot JM, Heal DJ, Marsden CA, editors. Experimental approaches to anxiety and depression. New York: Wiley, 1992:25–44.Google Scholar

  • 25.

    Del Vecchio S, Gargiulo PA. Visual and motor function in schizophrenic patients. Acta Psiquiát Psicol Am Lat 1992;38:317–22.Google Scholar

  • 26.

    Gargiulo PA. Experimental approaches to perceptual dysfunction in schizophrenia. Rev Neurol 2003;37:545–51.PubMedGoogle Scholar

About the article

aA.P.I. Gargiulo and M.P. Gargiulo De Aranda contributed equally to this article.


Received: 2017-07-03

Accepted: 2017-12-16

Published Online: 2018-02-14

Published in Print: 2018-06-27


Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: Funding research was received from Volkswagen Foundation, the Latin American Technological Corporation Foundation (Fucotel) and the National University of Cuyo (see aknowledgements).

Employment of leader: Pascual Ángel Gargiulo is Established Researcher of the National Council of Research (CONICET) and Professor of Pharmacology at the National University of Cuyo, Mendoza, Argentina.

Honorarium: No special honoraries were received by P.A. Gargiulo for present study.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, Volume 29, Issue 3, Pages 241–246, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2017-0100.

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