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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu / Tam, Joseph


CiteScore 2016: 1.01

SCImago Journal Rank (SJR) 2016: 0.349
Source Normalized Impact per Paper (SNIP) 2016: 0.495

Online
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2191-0286
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Volume 30, Issue 1

Issues

Local inhibition of carbonic anhydrase does not decrease sweat rate

Jeff Moore / Sarah Northway / Nicole Wells / Emily Woolf / Michael J. Buono
Published Online: 2018-08-13 | DOI: https://doi.org/10.1515/jbcpp-2018-0039

Abstract

Background:

The purpose of this study was to measure sweat rate during exercise in the heat after directly inhibiting carbonic anhydrase (CA) in eccrine sweat glands via transdermal iontophoresis of acetazolamide. It was hypothesized that if CA was important for sweat production, local administration of acetazolamide, without the confounding systemic effects of dehydration typically associated with past studies, would have a significant effect on sweat rate during exercise.

Methods:

Ten healthy subjects volunteered to exercise in the heat following acetazolamide or distilled water iontophoresis on the forearm.

Results:

The distilled water iontophoresis site had a mean sweat rate during exercise in the heat of 0.59±0.31 μL/cm2/min, while the acetazolamide iontophoresis site had a mean sweat rate of 0.63±0.36 μL/cm2/min (p>0.05).

Conclusions:

The most important finding of the current study was that iontophoresis of acetazolamide did not significantly decrease sweat rate during exercise in the heat. Such results suggest that in past studies it was systemic dehydration, and not CA inhibition at the level of the sweat gland, that caused the reported decreased sweat rate.

Keywords: carbonic anhydrase; dehydration; diamox; iontophoresis

References

  • 1.

    Briggman JV, Tashian RE, Spicer SS. Immunohistochemical localization of carbonic anhydrase I and II in eccrine sweat glands from control subjects and patients with cystic fibrosis. Am J Pathol 1983;112:250–7.PubMedGoogle Scholar

  • 2.

    Clunes MT, Lindsay SL, Roussa E, Quinton PM, Bovell DL. Localization of the vacuolar proton pump (V-H+-ATPase) and carbonic anhydrase II in the human eccrine sweat gland. J Mol Histol 2004;35:339–45.Google Scholar

  • 3.

    Cui CY, Schlessinger D. Eccrine sweat gland development and sweat secretion. Exp Dermatol 2015;24:644–50.CrossrefWeb of SciencePubMedGoogle Scholar

  • 4.

    Bovell D. The human eccrine sweat gland: structure, function, and disorders. J Local Glob Health Sci 2015;5:1–16.Google Scholar

  • 5.

    Dahlborn K, Jansson A, Nyman S, Morgan K, Holm L, Ridderstråle Y. Sweat production and localization of carbonic anhydrase in the equine sweat gland during exercise at two ambient temperatures. Eq Veter J 1999;30(S30):398–403.Google Scholar

  • 6.

    Sato K, Gisolfi CV, Lamb DR, Nadel ER. Perspectives in exercise science and sports medicine. Exercise, heat, and thermoregulation, Vol. 6. Indianapolis, IN: Benchmark Press, 1993:85–118.Google Scholar

  • 7.

    Brechue WF, Stager JM. Acetazolamide alters temperature regulation during submaximal exercise. J Appl Physiol 1990;69:1402–7.CrossrefPubMedGoogle Scholar

  • 8.

    Brechue WF, Stager JM, Lukaski HC. Body water and electrolyte responses to acetazolamide in humans. J Appl Physiol 1990;69:1397–401.CrossrefPubMedGoogle Scholar

  • 9.

    Ekblom B, Greenleaf CJ, Greenleaf JE, Hermansen L. Temperature regulation during exercise dehydration in man. Acta Physiol Scand 1970;79:475–83.PubMedCrossrefGoogle Scholar

  • 10.

    Fortney SM, Nadel ER, Wenger CB, Bove JR. Effect of blood volume on sweating rate and body fluids in exercising humans. J Appl Physiol 1981;51:1594–600.CrossrefGoogle Scholar

  • 11.

    Greenleaf JE, Castle BL. Exercise temperature regulation in man during hypohydration and hyperhydration. J Appl Physiol 1971;30:847–53.CrossrefPubMedGoogle Scholar

  • 12.

    Sawka M, Young A, Francesconi R, Muza S, Pandolf K. Thermoregulatory and blood pressure responses during exercise at graded hypohydration levels. J Appl Physiol 1985;59:1394–401.CrossrefGoogle Scholar

  • 13.

    Kaiser D, Songo-Williams R, Drack E. Hydrogen ion and electrolyte excretion of the single human sweat gland. Pflugers Archiv 1974;349:63–72.CrossrefGoogle Scholar

  • 14.

    Buono MJ, Wall AJ. Effect of hypohydration on core temperature during exercise in temperate and hot environments. Pflugers Archiv 2000;440:476–80.CrossrefGoogle Scholar

  • 15.

    Sato K. The physiology, pharmacology, and biochemistry of the eccrine sweat gland. Rev Physiol Biochem Pharmacol 1977;79:51–131.CrossrefPubMedGoogle Scholar

About the article

Corresponding author: Michael J. Buono, PhD, School of Exercise and Nutritional Sciences and the Department of Biology, San Diego State University, Mail Code: 7251, San Diego, CA 92182, USA, Phone: +619-594-6823


Received: 2018-02-27

Accepted: 2018-06-15

Published Online: 2018-08-13

Published in Print: 2018-12-19


Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, Volume 30, Issue 1, Pages 47–50, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2018-0039.

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