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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

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An isobolographic analysis of the anti-nociceptive effect of geraniin in combination with morphine or diclofenac

Eric Boakye-Gyasi
  • Corresponding author
  • Faculty of Pharmacy and Pharmaceutical Science, Department of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
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/ Ella Anle Kasanga
  • Faculty of Pharmacy and Pharmaceutical Science, Department of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
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/ Elvis Ofori Ameyaw / Wonder Kofi Mensah Abotsi
  • Faculty of Pharmacy and Pharmaceutical Science, Department of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
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/ Robert Peter Biney
  • Department of Pharmacology, School of Medical Sciences, University of Cape Coast, Cape Coast, Ghana
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/ Christian Agyare
  • Faculty of Pharmacy and Pharmaceutical Science, Department of Pharmaceutics, Kwame Nkrumah University of Science and Technology, KNUST, Kumasi, Ghana
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/ Eric Woode
  • Faculty of Pharmacy and Pharmaceutical Science, Department of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
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Published Online: 2017-10-09 | DOI: https://doi.org/10.1515/jbcpp-2017-0031

Abstract

Background:

Geraniin, a dehydroellagitannin, is a major component of the aqueous extract of the aerial parts of Phyllanthus muellerianus (Kuntze) Exell. (Euphorbiaceae). Several Phyllanthus species are traditionally used for painful disorders. The anti-nociceptive effects of the aqueous extract of the aerial parts of P. muellerianus and of geraniin have been scientifically established. The aim of the paper is to determine whether a combination of geraniin and diclofenac or geraniin and morphine leads to better anti-nociceptive effects.

Methods:

The nature of the interactions of morphine and diclofenac with geraniin was evaluated by undertaking the isobolographic analysis. Mice were treated with geraniin (3–30 mg/kg), morphine (1–10 mg/kg), and diclofenac (10–100 mg/kg) to obtain the ED50 values of the agents in the formalin test. Dose-response curves were then obtained and analyzed after the co-administration of geraniin with morphine or diclofenac in fixed ratio (1:1) combinations based on specific fractions (1/2, 1/4, and 1/8) of their respective ED50 values for the formalin test.

Results:

Geraniin was less potent than morphine but more potent than diclofenac in the formalin-induced nociception. The isobolographic analysis of geraniin/morphine (G/M) and geraniin/diclofenac combinations (G/D) at different fractions revealed the potentiation of their anti-nociceptive effects. The degrees of potentiation, which were calculated as interaction indices, showed synergism for both combinations in both phase I (G/M: 0.040, G/D: 0.017) and phase II (G/M: 0.004, G/D: 0.002) of the formalin test.

Conclusions:

The present study demonstrates synergism for the co-administration of geraniin with both morphine and diclofenac.

Keywords: analgesia; nociception; synergism

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About the article

Corresponding author: Dr. Eric Boakye-Gyasi, Faculty of Pharmacy and Pharmaceutical Science, Department of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, Tel.: (+233)-244635194, Fax: +233-322-60366


Received: 2017-03-13

Accepted: 2017-08-10

Published Online: 2017-10-09


Disclosure of interest: The authors report no conflicts of interest.

Funding: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.


Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, 20170031, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2017-0031.

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