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Journal of Complementary and Integrative Medicine

Editor-in-Chief: Lui, Edmund

Ed. by Ko, Robert / Leung, Kelvin Sze-Yin / Saunders, Paul / Suntres, PH. D., Zacharias

CiteScore 2017: 1.41

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Phytochemical screening and toxicity studies on the methanol extract of the seeds of moringa oleifera

Temitayo Olabisi Ajibade
  • Corresponding author
  • Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria
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/ Ruben Arowolo
  • University of Ibadan - Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria
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/ Funsho Olakitike Olayemi
Published Online: 2013-06-12 | DOI: https://doi.org/10.1515/jcim-2012-0015

Abstract: The seeds of Moringa oleifera were collected, air-dried, pulverized, and subjected to cold extraction with methanol. The methanol extract was screened phytochemically for its chemical components and used for acute and sub-acute toxicity studies in rats. The phytochemical screening revealed the presence of saponins, tannins, terpenes, alkaloids, flavonoids, carbohydrates, and cardiac glycosides but the absence of anthraquinones. Although signs of acute toxicity were observed at a dose of 4,000 mg kg1 in the acute toxicity test, and mortality was recorded at 5,000 mg kg1, no adverse effect was observed at concentrations lower than 3,000 mg kg1. The median lethal dose of the extract in rat was 3,873 mg kg1. Sub-acute administration of the seed extract caused significant (p<0.05) increase in the levels of alanine and aspartate transferases (ALT and AST), and significant (p<0.05) decrease in weight of experimental rats, at 1,600 mg kg1. The study concludes that the extract of seeds of M. oleifera is safe both for medicinal and nutritional uses.

Keywords: phytochemical screening median lethal dose; Moringa oleifera seeds


  • 1.

    Jahn SAA. Using Moringa oleifera seeds as coagulant in developing countries. J Am Water Works Assoc 1988;6:43–50.Google Scholar

  • 2.

    Kalogo Y, Rosillon F, Hammes F, Verstraete W. Effect of a water extract of Moringa oleifera seeds on the hydrolytic microbial species diversity of a UASB reactor treating domestic wastewater. Lett Appl Microbiol 2000;31:259–64.CrossrefGoogle Scholar

  • 3.

    Ndabigengesere A, Narasiah C. Quality of water treated by coagulation using Moringa oleifera seed. Water Resour 1998;32:781–91.Google Scholar

  • 4.

    Muyibi SA, Evison LM. Moringa oleifera seeds for softening hard water. Water Res 1995;29:1099–105.CrossrefGoogle Scholar

  • 5.

    Gilani AH, Rahman A. Trends in ethnopharmacology. J Ethnopharmacol 2005;100:43–9.CrossrefGoogle Scholar

  • 6.

    Oliveira JTA, SB Silveira, et al. (1999) Compositional and nutritional attributes of seeds from the multiple purpose tree Moringa oleifera Lamarck. Journal of the Science of Food and Agriculture 79(6): 815–820.Google Scholar

  • 7.

    Lorke D. A new method for acute toxicity testing. Arch Toxic 1983;79:117–43.Google Scholar

  • 8.

    Farris EJ, Griffith JQ, editors. The rat in laboratory investigation. New York: Hofner Publishing, 1949.Google Scholar

  • 9.

    Njar VCO, Alao TO, Okogun JI, Holland HL. 2 methoxy cathin-6-one: a new alkaloid from the stem wood of Quassia amara. Plant Med 1993;59:259–61.Google Scholar

  • 10.

    Sofowora AC. Medicinal Plants and Traditional Medicine in Africa. Ibadan: Spectrum Books, 1993:261–68.Google Scholar

  • 11.

    Evans WC. Trease and Evans’ pharmacognosy, 15th ed. Saunders, Elsevier (A Division of Reed Elsevier India Pvt. Limited), 2002:221–27, 229–31, 304–05, 336.Google Scholar

  • 12.

    Duncan JR, Prasse KW, Mahaffey EA. Veterinary Laboratory Medicine (Clinical Pathology). Ames, IA: Iowa State University Press, 1994:94–6.Google Scholar

  • 13.

    Bennett RN, Mellon FA, Foidl N, Pratt JH, Dupont MS, Perkins L, Kroon PA. Profiling glucosinolates and Phenolics in Vegetative and Reproductive tissues of the multi-purpose trees Moringa oleifera L. (Horseradish tree) and Moringa stenppetala L. J Agric Food Chem 2003;51:3546–53.CrossrefGoogle Scholar

  • 14.

    Olayemi AB, Alabi RO. Studies on traditional water purification using Moringa oleifera seeds. Afr Study Monogr 1994;15:135–42.Google Scholar

  • 15.

    Kam PCA, Liew S. Traditional Chinese herbal medicine and anaesthesia. Anaesthesia 2002;57: 1083–9.Google Scholar

  • 16.

    Nobori T, Miurak K, Wu DJ, Takabayashik LA, Carson DA. Deletion of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancers. Nature 1994;368:753–6.CrossrefGoogle Scholar

  • 17.

    Oladele SB, Ayo JO, Aaudi, AO. Medicinal and physiological properties of flavonoids, coumarin derivatives and anthraquinones of plant origin. West Africa J Pharm Drug Res 1995;11:134–44.Google Scholar

  • 18.

    Li M, Wang Y, Wah H, Tian C. Hypoglycemic effects of saponins from Tribulus terrestis. J Nat Prod 2002;25:420–2.Google Scholar

  • 19.

    Isaac OO, Chinwe JA. The phytochemical analysis and antibacterial screening of extract of Tetrecarpidum conophorum. J Chem Soc Niger 2001;26:53–5.Google Scholar

  • 20.

    Abuye C, Omwega AM, Imungi JK. Familial tendency and dietary association of goitre in Gamo-Gofa, Ethiopia. East Afr Med J 1999;76:447–51.Google Scholar

  • 21.

    Anwar F, and MI Bhanger (2003) Analytical characterization of Moringa oleifera seed oil grown in temperate regions of Pakistan. Journal of Agricultural and Food Chemistry 51: 6558–6563.CrossrefGoogle Scholar

  • 22.

    Shimada T. Salivary proteins as a defense against dietary tannins. J Chem Ecol 2006;32:1149–63.Web of ScienceCrossrefGoogle Scholar

  • 23.

    Parekh J, Chanda S. In vitro antibacterial activity of crude methanol extract of Woodfordia fruticosa Kurz flower (Lythacease). Braz J Microbiol 2007;38:2.Web of ScienceGoogle Scholar

  • 24.

    Dharamananda S. Gallnuts and the uses of Tannins in Chinese Medicine. In: Proceedings of Institute for Traditional Medicine, Portland, OR, 2003.Google Scholar

  • 25.

    Mbagwu FN, Okafor VU, Ekeanyanwu J. Phytochemical screening on four edible legumes (Vigna subterranean, Glycine max, Arachis hypogea, and Vigna uniguiculata) found in eastern Nigeria. Afr J Plant Sci 2011;5:370–2.Google Scholar

  • 26.

    Miller AL. Antioxidant flavonoids: structure, function and clinical usage. Alt Med Rev 1996;1:103–11.Google Scholar

  • 27.

    Ayoola GA, Ipav SS, Sofidiya MO, Adepoju-Bello AA, Coker HAB, Odugbemi TO. Phytochemical screening and free radical scavenging activities of the fruits and leaves of Allanblackia floribunda Oliv (Guttiferae). Int J Health Res 2008;1:87–93.Google Scholar

  • 28.

    Coppo JA, Mussart NB, Fioranelli SA. Physiological variations of enzymatic activities in blood of Bullfrog, Rana catesbeina (Shaw, 1802). Rev Vet 2002;12:22–7.Google Scholar

  • 29.

    Patti M, Kulkarni RS. Ovarian and hepatic biochemical response to Sumaach (a crude form of HCG) in fish, Notopterus notopterus pallas, under pesticide treatment. Geobios 1993;20:255–9.Google Scholar

  • 30.

    Bush BM (1991). Interpretation of laboratory results for small animal clinicians. Blackwell Scientific Publications: London, 56–59.Google Scholar

  • 31.

    Bain PJ. Liver. In: Latimer KS, Mahaffrey EA, Prasse KW, editors. Duncan and Prasse’s Veterinary Laboratory Medicine: Clinical Pathology, 4th ed. Ames, IA: Iowa State Press, 2003:193–414.Google Scholar

  • 32.

    Rodwell VW, Kennelly PJ. Enzymes: mechanism of action In: Murray RK, Granner DK, Mayes PA, Rodwell VW, editors. Harper’s illustrated biochemistry, 26th ed. New York: Lange Medical Books/McGraw-Hill, 2003:chap 7, 57.Google Scholar

About the article

Received: 2012-04-06

Accepted: 2012-11-20

Published Online: 2013-06-12

Citation Information: Journal of Complementary and Integrative Medicine, Volume 10, Issue 1, Pages 11–16, ISSN (Online) 1553-3840, ISSN (Print) 2194-6329, DOI: https://doi.org/10.1515/jcim-2012-0015.

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