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Journal of Complementary and Integrative Medicine

Editor-in-Chief: Lui, Edmund

Ed. by Ko, Robert / Leung, Kelvin Sze-Yin / Saunders, Paul / Suntres, PH. D., Zacharias

CiteScore 2017: 1.41

SCImago Journal Rank (SJR) 2017: 0.472
Source Normalized Impact per Paper (SNIP) 2017: 0.564

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Antimalarial potential of China 30 and Chelidonium 30 in combination therapy against lethal rodent malaria parasite: Plasmodium berghei

Aswathy Rajan / Upma Bagai
Published Online: 2013-06-15 | DOI: https://doi.org/10.1515/jcim-2012-0016

Abstract: Homeopathy is a therapeutic method based on the application of similia principle, utilizing ultra-low doses of medicinal substances made from natural products. The present study has been designed to evaluate the efficacy of Cinchona officinalis (Chin.) 30C and Chelidonium majus (Chel.) 30C in combination therapy against lethal murine malaria. Five groups having twelve BALB/c mice each were administered orally with 0.2 ml/mouse/day of different drugs, and their antimalarial potential was evaluated by Peter’s 4-day test. The combination of Chin. 30 and Chel. 30 exhibited complete parasite clearance by the 28th day post-inoculation which was similar to the positive control [artesunate (4 mg/kg)+sulphadoxine-primethamine (1.2 mg/kg)] group. Both the groups exhibited enhanced mean survival time (MST) 28±0 days,whereas, the mice of infected control group survived up to 7.6±0.4 days only. The preventive and curative activities of the combination in comparison to the positive controls [pyrimethamine (1.2 mg/Kg) and chloroquine (20 mg/Kg), respectively] were also evaluated. The combination had a significant preventive activity (p<0.0005), with 89.2% chemosuppression which was higher than the standard drug, pyrimethamine (83.8%). It also showed a moderate curative activity with complete clearance of parasite in 50% of surviving mice, and enhancing the MST of mice up to 26.8±2.8 days. These findings point to the significant antiplasmodial efficacy of the combination of these homeopathic drugs against Plasmodium berghei.

Keywords: homeopathy; Plasmodium berghei; Cinchona; Chelidonium majus


  • 1.

    World Health Organisation. World malaria report 2011. Geneva: WHO Press, 2011. ISBN 978 92 4 156440 3.Google Scholar

  • 2.

    Khuda-Bukhsh AR. Towards understanding molecular mechanisms of action of homeopathic drugs: an overview. Mol Cell Biochem 2003;253:339–45.Google Scholar

  • 3.

    Bhattacharyya SS et al. In vitro studies demonstrate anticancer activity of an alkaloid of plant Gelsemium semipervirens. Exp Biol Med 2008;233:1591–601.Web of ScienceGoogle Scholar

  • 4.

    Khuda-Bukhsh AR. Mice as a model for homeopathy research. Homeopathy 2009;98:267–79.CrossrefWeb of ScienceGoogle Scholar

  • 5.

    Salazar GL et al. Effects of homeopathic medications Eupatorium perfoliatum and Arsenicum album on parasitaemia of Plasmodium berghei infected mice. Homeopathy 2006;95:223–8.Google Scholar

  • 6.

    Rajan A, Bagai U. SEM studies on blood cells of Plasmodium berghei infected Balb/c mice treated with artesunate and homeopathic medicine china. J Parasit Dis 2011;35:134–9.CrossrefGoogle Scholar

  • 7.

    Rajan A, Bagai U. Evaluation of antiplasmodial efficacy and safety of Cinchona officinalis against lethal murine malaria parasite. Am J Homeopath Med 2012;105:76–83.Google Scholar

  • 8.

    Dubey SK. Text book of materia medica [including Allen’s keynote- an easy explanation]. New Delhi: Books and Allied, 2007:1–184.Google Scholar

  • 9.

    Gonuguntla SR. The science behind homeopathy-unraveling the mystery: an allopathic doctor now extensively researching on homeopathy. Homeopathy 4 Everyone 2010;7:2–5.Google Scholar

  • 10.

    Rajan A, Bagai U. Effect of homeopathic medicine Chelidonium against blood stage infection of rodent malaria parasite. In: Advances in parasitology: a novel approach towards a disease free world, Proceedings of the 22nd National Congress of Parasitology, October 30–November 1, 2010, University of Kalyani, West Bengal, India, 2011:27–31.Google Scholar

  • 11.

    Taborska E et al. The greater celandine (Chelidonium majus L.) – a review of present knowledge. Ceska Slov Farm 1995;44:71–5.Google Scholar

  • 12.

    Partington T et al. Silent and deadly, prophylaxis and treatment of malaria. Homeopath Pract 2006;Spring:14–19.Google Scholar

  • 13.

    Santiyanont R. Parasite identification, counting and staining. In: Application of genetic engineering techniques in tropical diseases, pathogens with special reference to plasmodia. A laboratory manual of selected techniques. UNDP/World Bank/WHO special program for research and techniques in tropical diseases, Bangkok, Thailand, 1985, 8.7:413–48.Google Scholar

  • 14.

    Knight DJ, Peters W. The antimalarial action of benzyloxydihydrotriazines. The action of Cycloguanil (BRL50216) against rodent malaria and studies on its mode of action. Ann Trop Med Parasitol 1980;84:393–404.Google Scholar

  • 15.

    Peters W. Drug resistance in Plasmodium berghei. Exp Parasitol 1965;17:80–9.CrossrefGoogle Scholar

  • 16.

    Ryley JF, Peters W. The antimalarial activity of some quinolone esters. Ann Trop Med Parasitol 1970;74:209–22.Google Scholar

  • 17.

    Wijk MV. Importance of the right potency: role of potency selection I homeopathy. Homeopathy 4 Everyone 2012;9:23–4.Google Scholar

  • 18.

    Bagai U et al. Efficacy of Eucalyptus (a homeopathic medicine) in combination with artesunate to clear Plasmodium berghei infection in Balb/C mice. In: Current trends in parasitology, Proceedings of the 20th National Congress of Parasitology, November 3–5, 2008, NEHU, Shillong, India, 2008:203–11.Google Scholar

  • 19.

    Rajan A et al. Effect of artesunate based combination therapy with homeopathic medicine china on liver and kidney of Plasmodium berghei infected mice. J Parasit Dis 2012. DOI:–011–0059y.CrossrefGoogle Scholar

  • 20.

    Rajan A. Efficacy of some homeopathic medicines against rodent malaria parasite Plasmodium berghei. Ph.D. Thesis, Panjab University, Chandigarh, 2012.Google Scholar

  • 21.

    Georgewill UO, Ebong OO. A comparative study on the efficacy of some artemisinin combination therapies on Plasmodium berghei in Swiss albino mice. Pharmacol Pharm 2012;3:109–12.CrossrefGoogle Scholar

  • 22.

    Van VMA, Brands M. Malaria and homeopathic remedies in Ghana, an open study and a double-blind randomized clinical trial. Homeopathy 1993;5:312–16.Google Scholar

  • 23.

    Bagai U et al. Antimalarial potential of nosode 30 and 200 against Plasmodium berghei infection in Balb/c mice. J Vector Borne Dis 2012;49:72–7.Google Scholar

  • 24.

    Davis EE et al. The chemotherapy of rodent malaria action of quinine and WR 122–445, the fine structure in Plasmodium berghei in mouse blood. Ann Trop Med Parasitol 1975;69:147–55.Google Scholar

  • 25.

    Lessell C. Handbook of the homeopathic traveler’s manual. Winter Press, UK, 1993:21–33.Google Scholar

  • 26.

    Shah H. Malaria and homeopathy. New Delhi: Mayur Jain, Indian Books and Periodicals Publishers, 1999:1–233.Google Scholar

  • 27.

    Adler M et al. Effects of Chelidonium majus extracts in human hepatocytes in vitro. Toxicol Lett 2006;164S:15–16.Google Scholar

  • 28.

    Dow GS et al. Plasmodium berghei: a new rat model for assessment of blood schizonticidal activity. Exp Parasitol 1999;93:92–4.Google Scholar

  • 29.

    Abolghasemi E et al. Comparative study of chloroquine and quinine on malaria rodents and their effects on the mouse testis. Asian Pac J Trop Biomed 2012:311–14.Google Scholar

  • 30.

    Arnstein PRN. The placebo effect. Seminars in Integ Med 2003;1:125–35.Google Scholar

About the article

Received: 2012-09-11

Accepted: 2013-03-08

Published Online: 2013-06-15

Citation Information: Journal of Complementary and Integrative Medicine, Volume 10, Issue 1, Pages 89–96, ISSN (Online) 1553-3840, ISSN (Print) 2194-6329, DOI: https://doi.org/10.1515/jcim-2012-0016.

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