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Journal of Complementary and Integrative Medicine

Editor-in-Chief: Lui, Edmund

Ed. by Ko, Robert / Leung, Kelvin Sze-Yin / Saunders, Paul / Suntres, PH. D., Zacharias


CiteScore 2017: 1.41

SCImago Journal Rank (SJR) 2017: 0.472
Source Normalized Impact per Paper (SNIP) 2017: 0.564

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1553-3840
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Chemical and pharmacological standardization of Ashwagandhadi lehyam: an ayurvedic formulation

Arun Rasheed
  • Corresponding author
  • Department of Pharmaceutical Chemistry, Al Shifa College of Pharmacy, Poonthavanam P.O., Kizhattur, Perinthalamanna, Malappuram, Kerala, India
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/ K.V. Satyanarayana
  • Department of Pharmacognosy and Phytochemistry, Sree Vidyanikethan College of Pharmacy, Tirupati, Andhra Pradesh, India
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/ P. Swetha Gulabi
  • Department of Pharmacognosy and Phytochemistry, Sree Vidyanikethan College of Pharmacy, Tirupati, Andhra Pradesh, India
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/ M. Sindhura Rao
  • Department of Pharmacognosy and Phytochemistry, Sree Vidyanikethan College of Pharmacy, Tirupati, Andhra Pradesh, India
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Published Online: 2013-10-15 | DOI: https://doi.org/10.1515/jcim-2012-0026

Abstract

Standardization is an important measurement for ensuring the quality control of herbal drugs. It has become essential to develop reliable, specific and sensitive quality control methods. One of the prime ingredients of Ashwagandhadi lehyam is Withania somnifera L. Dunal (Solanaceae) (ashwagandha). In the present study, Ashwagandhadi lehyam was formulated and the quality assessment of the formulation was based on phytochemical screening and physico-chemical evaluation. Ashwagandhadi lehyam was formulated according to a working formula and subjected to phytochemical screening by FTIR analysis and HPTLC fingerprinting, heavy metal determination by AAS, determination of alcohol content, tested for Escherichia coli, Staphylococcus aureus, aerobic bacteria, yeasts and mould, oral toxicity studies and anti-epileptic activity by MES method. The physico-chemical studies showed total ash content as 6.45%, extractive values and some trace elements such as lead, mercury, cadmium and arsenic with 3.2, 0.05, 0.18 and 0.48 ppm, respectively. FTIR and HPTLC studies revealed the presence of functional groups of withanolides in Ashwagandhadi lehyam, resulting in its chemical standardization. The formulation exhibited less epileptic seizures in various phases when compared with that of standard phenytoin and found to possess better anti-inflammatory activity, thus making it biologically standardized. The physico-chemical and pharmacological analysis to standardize Ashwagandhadi lehyam confirmed its use as a safe anti-inflammatory agent and for various seizure disorders.

Keywords: Ashwagandhadi lehyam; standardization; anti-inflammatory activity; anti-epileptic activity; quality control

References

  • 1.

    Rasheed A, Satyanarayana KV, Swetha Gulabi P, Sindhura M, Neelufar Shama S, Avinash Kumar RG. Standardization: accessing key for herbal drug safety assurance. J Pharm Res 2011;4:1322–5.Google Scholar

  • 2.

    Gupta M, Bisht D, Pandey MM, Ojha SK, Khatoon S, Rastogi S, et al. Standardization of Ashwagandhadi lehya – an important Ayurvedic formulation of Withania sominifera. Ind J Trad Know 2011;10:594–8.Google Scholar

  • 3.

    Mukharjee PK. Quality control of herbal drugs-An approach to evaluation of botanicals. New Delhi, India: Business Horizons Pharmaceutical Publications, 2002.Google Scholar

  • 4.

    Ayurvedic formulary of India Part-1. 2nd revised Ed. Ministry of health and family welfare. New Delhi, India: Government of India, 2003:108–13.Google Scholar

  • 5.

    Gupta MK, Sharma PK. Text book of pharmacognosy. Meerut, India: Pragati Prakashan Limited, 2007.Google Scholar

  • 6.

    Nadkarni KM. Indian materia medica. Bombay, India: Popular Prakashan Limited, 1976.Google Scholar

  • 7.

    Mohammad HM, Moyano E, Bonfill M, Cusido RM, Palazon J. Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecules 2009;14:2373–93.Web of ScienceGoogle Scholar

  • 8.

    World Health Organization. Quality control methods for medicinal plant materials. Geneva: WHO, 2007.Google Scholar

  • 9.

    Indian Pharmacopoeia. Ministry of health and family welfare. New Delhi, India: Government of India, 1996.Google Scholar

  • 10.

    Harborne JB. Phytochemical methods – a guide to modern techniques of plant analysis. New York: Chapman and Hall, 1984.Google Scholar

  • 11.

    Wagner H, Bladt S. Plant drug analysis: a thin layerchromatography atlas. Berlin, Heidelberg: Springer-Verlag, 1996.Google Scholar

  • 12.

    Kalaiselvan V, Shah AK, Patel FB, Shah CN, Kalaivani M, Rajasekharan A. Quality assessment of different marketed brands of Dasamoolaristam, an Ayurvedic formulation. Int J Ayur Res 2010;1:10–13.CrossrefGoogle Scholar

  • 13.

    Chattopadhyay RN, Chattopadhyay R, Roy S, Moitra SK. A simple method for plethysmometric measurement of paw volume of small laboratory animals in evaluation of anti inflammatory effects. Bull Calcutta School Trop Med 1986;34:5–8.Google Scholar

  • 14.

    Swinyard EA. Laboratory evaluation of antiepileptic drugs. Epilepsia 1969;10:107–19.CrossrefPubMedGoogle Scholar

About the article

Received: 2012-02-15

Accepted: 2013-09-15

Published Online: 2013-10-15


Citation Information: Journal of Complementary and Integrative Medicine, Volume 10, Issue 1, Pages 1–9, ISSN (Online) 1553-3840, ISSN (Print) 2194-6329, DOI: https://doi.org/10.1515/jcim-2012-0026.

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