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Licensed Unlicensed Requires Authentication Published by De Gruyter February 6, 2014

Saffron in metabolic syndrome: its effects on antibody titers to heat-shock proteins 27, 60, 65 and 70

  • Maryam Shemshian , Seyed Hadi Mousavi , Abdolreza Norouzy , Tayebe Kermani , Toktam Moghiman , Akram Sadeghi , Majid Ghayour-Mobarhan EMAIL logo and Gordon A. Ferns

Abstract

Background: The metabolic syndrome is the most important risk factor for cardiovascular disease. The heat shock proteins (HSPs) are highly conserved families of proteins expressed by a number of cell types following exposure to stressful environmental conditions include several known risk factors for cardiovascular disease. Recent studies have shown the potential of constituents of saffron in the treatment of atherosclerosis. We aimed on investigating the effect of saffron on antibody titers to HSP in patients with metabolic syndrome.

Methods: This was a randomized, placebo-controlled clinical trial. One-hundred and five subjects with metabolic syndrome were randomly allocated to one of the three groups: the case group received 100 mg/day saffron, the placebo control group received a capsule of placebo and a non-placebo control group received no capsule, for 12 weeks.

Results: Antibodies against heat shock proteins 27, 60, 65 and 70 were determined in all patients before (week 0) and after (week 6 and 12) intervention. At 12 weeks, saffron produced a significantly decrease in AntiHSP27, 70 levels. Saffron can decrease AntiHSP27, 70 levels in patients with metabolic syndrome.

Conclusions: The results of this study indicate the efficacy of saffron in the improvement of some markers of autoimmunity HSPs in patients with metabolic syndrome.


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Maryam Shemshian and Seyed Hadi Mousavi contributed equally to this work.


Acknowledgments

The authors wish to thank the Iran National Science Foundation, Birjand University of Medical Sciences and Mashhad University of Medical Sciences for their financial support in performing this trial. This work is part of Maryam Shemshian’s MSc thesis, grant number [A-323].

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research support played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Research Funding: None declared.

Employment or Leadership: None declared.

Honorarium: None declared.

References

1. XuH, BrillJA, HsienJ, McBrideR, BoulianneGL, TrimbleWS. Syntaxin 5 is required for cytokinesis and spermatid differentiation in drosophila. Dev Biol2002;251:294306.10.1006/dbio.2002.0830Search in Google Scholar PubMed

2. PourghadamyariH, MoohebatiM, ParizadehSM, FalsoleimanH, DehghaniM, FazlinezhadA, et al. Serum antibody titers against heat shock protein 27 are associated with the severity of coronary artery disease. Cell Stress Chaperones2011;16:30916.10.1007/s12192-010-0241-7Search in Google Scholar PubMed PubMed Central

3. NematyM, DahriM, TavallaieS, LotfianE, KhorashadizadehM, FernsG, et al. Effect of a high dairy diet on serum antibody titers to heat shock protein 27 in overweight and obese children. Iran J Pediatr2009;19:4146.Search in Google Scholar

4. SnoeckxLH, CornelussenRN, Van NieuwenhovenFA, RenemanRS, Van der VusseGJ. Heat shock proteins and cardiovascular pathophysiology. Physiol Rev2001;81:146197.10.1152/physrev.2001.81.4.1461Search in Google Scholar PubMed

5. MorimotoRI. Cells in stress: transcriptional activation of heat shock genes. Science1993;259:140910.10.1126/science.8451637Search in Google Scholar PubMed

6. HuittinenT, LeinonenM, TenkanenL, MänttäriM, VirkkunenH, PitkänenT, et al. Autoimmunity to human heat shock protein 60, chlamydia pneumoniae infection, and inflammation in predicting coronary risk. Arterioscler Thromb Vasc Biol2002;22:43137.10.1161/hq0302.104512Search in Google Scholar PubMed

7. KolA, BourcierT, LichtmanAH, LibbyP. Chlamydial and human heat shock protein 60s activate human vascular endothelium, smooth muscle cells, and macrophages. J Clin Invest1999;103:57177.10.1172/JCI5310Search in Google Scholar PubMed PubMed Central

8. XuQ, KiechlS, MayrM, MetzlerB, EggerG, OberhollenzerF, et al. Association of serum antibodies to heat-shock protein 65 with carotid atherosclerosis: clinical significance determined in a follow-up study. Circulation1999;100:116974.10.1161/01.CIR.100.11.1169Search in Google Scholar PubMed

9. XuQ, SchettG, PerschinkaH, MayrM, EggerG, OberhollenzerF, et al. Serum soluble heat shock protein 60 is elevated in subjects with atherosclerosis in a general population. Circulation2000;102:1420.10.1161/01.CIR.102.1.14Search in Google Scholar PubMed

10. BurianK, KisZ, VirokD, EndreszV, ProhaszkaZ, DubaJ, et al. Independent and joint effects of antibodies to human heat-shock protein 60 and chlamydia pneumoniae infection in the development of coronary atherosclerosis. Circulation2001;103:15038.10.1161/01.CIR.103.11.1503Search in Google Scholar PubMed

11. ZhuJ, QuyyumiAA, RottD, CsakoG, WuH, HalcoxJ, et al. Antibodies to human heat-shock protein 60 are associated with the presence and severity of coronary artery disease evidence for an autoimmune component of atherogenesis. Circulation2001;103:107175.10.1161/01.CIR.103.8.1071Search in Google Scholar

12. GrundySM, CleemanJI, DanielsSR, DonatoKA, EckelRH, FranklinBA, et al. Diagnosis and management of the metabolic syndrome an American heart association/national heart, lung, and blood institute scientific statement. Circulation2005;112:273552.10.1161/CIRCULATIONAHA.105.169404Search in Google Scholar

13. CameronAJ, ShawJE, ZimmetPZ. The metabolic syndrome: prevalence in worldwide populations. Endocrinol Metab Clin North Am2004;33:351.10.1016/j.ecl.2004.03.005Search in Google Scholar

14. RodbardHW, JellingerPS, DavidsonJA, EinhornD, GarberAJ, GrunbergerG, et al. Statement by an American association of clinical endocrinologists/American college of endocrinology consensus panel on type 2 diabetes mellitus: an algorithm for glycemic control. Endocr Pract2009;15:54059.10.4158/EP.15.6.540Search in Google Scholar

15. GongW, GottliebS, CollinsJ, BlesciaA, DietzH, GoldmuntzE, et al. Mutation analysis of TBX1 in non-deleted patients with features of DGS/VCFS or isolated cardiovascular defects. J Med Genet2001;38:e45.10.1136/jmg.38.12.e45Search in Google Scholar

16. SchmidtM, BettiG, HenselA. Saffron in phytotherapy: pharmacology and clinical uses. Wien Med Wochenschr2007;157:31519.10.1007/s10354-007-0428-4Search in Google Scholar

17. HosseinzadehH, YounesiHM. Antinociceptive and anti-inflammatory effects of Crocus sativus L.stigma and petal extracts in mice. BMC Pharmacol2002;2:7.10.1186/1471-2210-2-7Search in Google Scholar

18. AbdullaevFI. Cancer chemopreventive and tumoricidal properties of saffron (Crocus sativus L). Exp Biol Med2002;227:205.10.1177/153537020222700104Search in Google Scholar

19. AbdullaevF, Espinosa-AguirreJ. Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detect Prev2004;28:42632.10.1016/j.cdp.2004.09.002Search in Google Scholar

20. ZhengS, QianZ, TangF, ShengL. Suppression of vascular cell adhesion molecule-1 expression by crocetin contributes to attenuation of atherosclerosis in hypercholesterolemic rabbits. Biochem Pharmacol2005;70:11929.10.1016/j.bcp.2005.07.034Search in Google Scholar

21. KellyD, TiidusP, HoustonM, NobleE. Effect of vitamin E deprivation and exercise training on induction of HSP70. J Appl Physiol1996;81:237985.10.1152/jappl.1996.81.6.2379Search in Google Scholar

22. AndrésD, AlvarezAM, Díez-FernándezC, ZaragozaA, CascalesM. HSP70 induction by cyclosporine A in cultured rat hepatocytes: effect of vitamin E succinate. J Hepatol2000;33:5709.10.1016/S0168-8278(00)80009-4Search in Google Scholar

23. AndrésD, MaC. Novel mechanism of vitamin E protection against cyclosporine A cytotoxicity in cultured rat hepatocytes. Biochem Pharmacol2002;64:26776.10.1016/S0006-2952(02)01112-7Search in Google Scholar

24. KhassafM, McArdleA, EsanuC, VasilakiA, McArdleF, GriffithsR, et al. Effect of vitamin C supplements on antioxidant defence and stress proteins in human lymphocytes and skeletal muscle. J Physiol2003;549:64552.10.1113/jphysiol.2003.040303Search in Google Scholar PubMed PubMed Central

25. CarratelliCR, NuzzoI, VitielloT, GaldieroE, GaldieroF. The effect of dietary lipid manipulation on murine splenic lymphocytes apoptosis and heat shock protein over expression. FEMS Immunol Med Microbiol1999;24:1925.10.1111/j.1574-695X.1999.tb01260.xSearch in Google Scholar PubMed

26. Ghayour-MobarhanM, NewSA, LambDJ, StarkeyBJ, LivingstoneC, WangT, et al. Dietary antioxidants and fat are associated with plasma antibody titers to heat shock proteins 60, 65, and 70 in subjects with dyslipidemia. Am J Clin Nutr2005;81:9981004.10.1093/ajcn/81.5.998Search in Google Scholar PubMed

Received: 2013-9-19
Accepted: 2013-12-10
Published Online: 2014-2-6

©2014 by Walter de Gruyter Berlin / Boston

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