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Journal of Complementary and Integrative Medicine

Editor-in-Chief: Lui, Edmund

Ed. by Ko, Robert / Leung, Kelvin Sze-Yin / Saunders, Paul / Suntres, PH. D., Zacharias

CiteScore 2017: 1.41

SCImago Journal Rank (SJR) 2017: 0.472
Source Normalized Impact per Paper (SNIP) 2017: 0.564

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Studies on anti-inflammatory and analgesic properties of Lactobacillus rhamnosus in experimental animal models

Sarika Amdekar
  • Department of Microbiology, Barkatullah University, Hoshangabad Road, Bhopal 462026, Madhya Pradesh, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Vinod Singh
  • Corresponding author
  • Department of Microbiology, Barkatullah University, Hoshangabad Road, Bhopal 462026, Madhya Pradesh, India
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-04-14 | DOI: https://doi.org/10.1515/jcim-2015-0087


Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used for the treatment of inflammatory diseases. However, constant use of NSAID may lead to some side effects like gastrointestinal ulcers, bleeding and renal disorders. This study evaluates analgesic and anti-inflammatory activities of Lactobacillus rhamnosus in female Wistar rats.

Methods: Diclofenac sodium was used as a standard drug for comparison. L. rhamnosus, drugs and vehicle were administered orally. Acetic acid-induced writhing test and carrageenan-induced paw edema model were used for evaluation. Paw edema and number of writhes were measured subsequently. Pro-inflammatory (interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α and IL-17) and anti-inflammatory (IL-4 and IL-10) cytokines were estimated in serum after 24 h.

Results: Results showed that L. rhamnosus significantly decreased the paw thickness at t=24 h by 28.66 % while drug decreased by 19.33 %. Also, L. rhamnosus treatment and standard drug showed a protection of 66.66 % and 41.66 %, respectively. L. rhamnosus and diclofenac sodium treatment significantly down-regulated pro-inflammatory and up-regulated anti-inflammatory cytokines at p<0.0001. Overall, protection provided by L. rhamnosus was more pronounced in comparison to diclofenac sodium.

Conclusions: The present study clearly suggests that L. rhamnosus suppressed carrageenan-induced paw edema after second phase and decreased the acetic acid-induced writhings. It ameliorated the inflammatory pathways by down-regulating pro-inflammatory cytokines. However, additional clinical investigations are needed to prove the efficacy of L. rhamnosus in treatment/management of inflammatory joint diseases.

Keywords: analgesic activity; carrageenan model; Lactobacillus rhamnosus


  • 1. Oyedapo OA, Adewunmi CO, Iwalewa EO, Makanju VO. Analgesic, antioxidant and anti-inflammatory related activities of 21-hydroxy-2, 41-dimethoxychalcone and 4-hydroxychalcone in mice. J Biol Sci 2008;8:131–6.Google Scholar

  • 2. Sosa S, Balick MJ, Arvigo R, Esposito RG, Pizza C, Altinier G, et  al. Screening of the tropical anti-inflammatory activity of some Central American plants. J Ethnopharmacol 2002;81:211–15.Google Scholar

  • 3. Amdekar S, Singh V, Singh R, Sharma P, Keshav P, Kumar A. Lactobacillus casei reduces the inflammatory joint damage associated with collagen induced arthritis by reducing the pro-inflammatory cytokines. J Clin Immunol 2011;31:147–4.Google Scholar

  • 4. Tapiero H, Ba GN, Couvreur P, Tew KD. Poly-unsaturated fatty acids (PUFA) and eicosanoids in human health and pathologies. Biomed Pharmacother 2002;56:215–22.Google Scholar

  • 5. Ayoola GA, Alpanika GA, Awabajo FO, Sofidiya MO, Osunkalu VO, Coker HAB, et al. Anti-inflammatory properties of the fruits of Allanblanckia floribunda olive (Guttiferae). Bot Res Int 2009;2:21–6.Google Scholar

  • 6. WHO. Traditional medicine strategy, 2002–2005. Geneva, Switzerland: WHO, 2002.Google Scholar

  • 7. Roy P, Amdekar S, Kumar A, Singh V. Preliminary study of the antioxidant properties of flowers and roots of Pyrostegiavenusta (Ker Gawl) Miers. BMC Comp Alt Med 2011;11:Article 69.Google Scholar

  • 8. Barua CC, Talukdar A, Begum SA, Lahon LC, Sarma DK, Pathak DC, et al. Antinociceptive activity of methanolic extract of leaves of Achyranthes aspera Linn. (Amaranthaceae) in animal models of nociception. Ind J Exp Biol 2010;48:817–21.Google Scholar

  • 9. Amdekar S, Roy P, Singh V, Kumar A, Singh R, Sharma P. Anti-inflammatory activity of Lactobacillus on carrageenan induced paw edema in male Wistar rats. Int J Inflamm 2012:Article ID 752015.

  • 10. Amdekar S, Singh V, Kumar A, Sharma P, Singh R. Lactobacillus casei and Lactobacillus acidophilus regulates inflammatory pathway and improves antioxidant status in collagen induced arthritic rats. J Interferon Cytokine Res 2013;33:1–8.Web of ScienceGoogle Scholar

  • 11. Winter CA, Risley EA, Nuss GW. Carrageenan-induced edema in the hind paw of rat as assays for anti-inflammatory activity. Proc Soc Exp Biol Med 1962;111:544–7.Google Scholar

  • 12. Koster R, Anderson M, De Beer J. Acetic acid for analgesic screening. Fed Pro 1959;18:412–17.Google Scholar

  • 13. Wills L. Release of histamin, kinin and prostaglandins during carrageenin induced inflammation of the rats. In: P Montagazza, EW Horton, editors. Prostaglandins, peptides and amines. London: Academic Press, 1969:31–48.Google Scholar

  • 14. Bhukya B, Anreddy RN, William CM, Gottumukkala KM. Analgesic and anti-inflammatory activities of leaf extract of Kydia calycina Roxb. Bang J Pharmacol 2009;4:101–4.Google Scholar

  • 15. Brooks PM, Day RO. Non-steroidal anti-inflammatory drugs difference and similarities. N Engl J Med 1991;324:1716–25.Google Scholar

  • 16. Giuliano F, Warner TD. Origins of prostaglandin E2: involvements of cyclooxygenase (COX)-1 and COX-2 in human and rat systems. J Pharmacol Exp Ther 2002;303:1001–6.Google Scholar

  • 17. Ouyang BS, Che JL, Gao J, Zhang Y, Li J, Yang HZ, et al. Effects of electro acupuncture and simple acupuncture on changes of IL-1, IL-4, IL-6 and IL-10 in peripheral blood and joint fluid in patients with rheumatoid arthritis. Zhongguo Zhen Jiu 2010;30:840–4.Google Scholar

  • 18. Ursaciuc C, Surcel M, Ciotaru D, Dobre M, Pirvu IR, Munteanu AN, et al. Regulatory T cells and TH1/TH2 cytokines as immunodiagnosis keys in systemic autoimmune diseases. Roumanian Arch Microbiol Immunol 2010;69:79–84.Google Scholar

  • 19. Ziolkowska M, Koc A, Luszczykiewicz G, Ksiezopolska-Pietrzak K, Klimczak E, Chwalinska-Sadowska H, et al. High levels of IL-17 in rheumatoid arthritis patients: IL-15 triggers in vitro IL-17 production via cyclosporin A-sensitive mechanism. J Immunol 2000;164:2832–8.Google Scholar

  • 20. Hwang SY, Kim JY, Kim KW, Park MK, Moon Y, Kim WU, et al. IL-17 induces production of IL-6 and IL-8 in rheumatoid arthritis synovial fibroblasts via NF-kB and PI3-kinase/Akt-dependent pathways. Arthritis Res Ther 2004;6:R120–28.Google Scholar

  • 21. Trushin SA, Pennington K, Carmona EM, Asin S, Savoy DN, Billadeau DD, et al. Protein kinase Cα (PKCα) acts upstream of PKCθ to activate IκB kinase and NF-κB in T lymphocytes. Mol Cell Biol 2003;23:7068–81.Google Scholar

  • 22. Baird-Lambert JD, Jamieson D. Possible mediators of the writhing response induced by acetic acid or phenylbenzoquinone in mice. Clin Exp Pharmacol Physiol 2007;10:15–20.Google Scholar

  • 23. Ribeiro RA, Vale ML, Thomazzi SM, Paschoalato AB, Poole S, Ferreira SH, et al. Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan and acetic acid in mice. Eur J Pharmacol 2000;387:111–18.Google Scholar

  • 24. Voilley N. Acid-sensing ion channels (ASICs): new targets for the analgesic effects of non-steroid anti-inflammatory drugs (NSAIDs). Curr Drug Targets 2004;3:71–9.Google Scholar

  • 25. Hossain MM, Ali MS, Saha A, Alimuzzaman Md. Antinociceptive activity of whole plant extracts of Paederia foetida. Dhaka Univ J Pharm Sci 2006;5:67–9.Google Scholar

  • 26. Solanki HK, Shah DA, Maheriya PM, Patel CA. Evaluation of anti-inflammatory activity of probiotic on carrageenan-induced paw edema in Wistar rats. Int J Biol Macromol 2015;72:1277–82.Web of ScienceGoogle Scholar

  • 27. Archer AC, Muthukumar SP, Halami PM, Anti-inflammatory potential of probiotic Lactobacillus spp. on carrageenan induced paw edema in Wistar rats. Int J Biol Macromol 2015;81:530–7.Web of ScienceGoogle Scholar

About the article

aSarika Amdekar and Vinod Singh contributed equally to this work.

Received: 2015-10-05

Accepted: 2016-02-08

Published Online: 2016-04-14

Published in Print: 2016-06-01

Citation Information: Journal of Complementary and Integrative Medicine, Volume 13, Issue 2, Pages 145–150, ISSN (Online) 1553-3840, ISSN (Print) 2194-6329, DOI: https://doi.org/10.1515/jcim-2015-0087.

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