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Journal of Complementary and Integrative Medicine

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Ed. by Ko, Robert / Leung, Kelvin Sze-Yin / Saunders, Paul / Suntres, PH. D., Zacharias

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Antidiabetic and in vitro antioxidant effects of hydromethanol extract of Paullinia pinnata root bark in alloxan-induced diabetic rat

Onoja Samuel OkwudiliORCID iD: http://orcid.org/0000-0001-6535-6110
  • Corresponding author
  • Department of Veterinary Physiology, Michael Okpara University of Agriculture Umudike, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, PMB 7267, Abia State Umuahia, Nigeria
  • Department of Biochemistry, Michael Okpara University of Agriculture, College of Natural Sciences, PMB 7267, Abia State Umuahia, Nigeria
  • orcid.org/0000-0001-6535-6110
  • Email
  • Other articles by this author:
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/ Nwachukwu Glory Chimaobi
  • Department of Biochemistry, Michael Okpara University of Agriculture, College of Natural Sciences, PMB 7267, Abia State Umuahia, Nigeria
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/ Ezeja Maxwell Ikechukwu
  • Department of Veterinary Physiology, Michael Okpara University of Agriculture Umudike, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, PMB 7267, Abia State Umuahia, Nigeria
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  • De Gruyter OnlineGoogle Scholar
/ Omeh Yusuf Ndukaku
  • Department of Biochemistry, Michael Okpara University of Agriculture, College of Natural Sciences, PMB 7267, Abia State Umuahia, Nigeria
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Published Online: 2017-11-17 | DOI: https://doi.org/10.1515/jcim-2015-0017



The study evaluated phytochemical composition, antidiabetic, oral glucose tolerance test and in vitro antioxidant activities of hydromethanol extract of Paullinia pinnata root bark.


Cold maceration method was used in extract preparation and scavenging of 2,2-diphenyl-1-picrylhydrazyl radicals was used to evaluate antioxidant properties of the extract. Diabetes was induced with alloxan at the dose of 160 mg/kg. The antidiabetic activity of the extract was tested at doses of 50, 100 and 200 mg/kg, and glibenclamide was used as reference drug.


Phytochemical analysis of the extract showed the presence of alkaloids, flavonoids, glycosides, tannins, saponins and terpenes/sterols. The extract produced a significant (p<0.05) time-dependent decrease in the fasting blood glucose (FBG) levels in the treated rats when compared with the distilled water treated rats, but did not produce dose-dependent effects. The extract 50, 100 and 200 mg/kg and glibenclamide (2 mg/kg) caused 83.62 %, 60.66 %, 47.77 % and 68.52 % reduction respectively in FBG at 6 h post-treatment while the distilled water (5 mL/kg) produced 8.12 % reduction in FBG at 6 h post treatment. The extract (50 mg/kg) and glibenclamide (2 mg/kg) produced a significant (p<0.05) oral glucose tolerance effect in both normoglycemic and diabetic rats. The extract produced concentration-dependent increase in antioxidant activity and had its optimum effect at 400 µg/mL concentration.


This study suggests that P. pinnata root bark has potent antidiabetic and antioxidant activities and also validates its use in folkloric medicine in the management of diabetes-related conditions.

Keywords: alloxan monohydrate; antioxidant; diabetes mellitus; oral glucose tolerance test; Paullinia pinnata


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About the article

Received: 2015-03-14

Accepted: 2017-10-02

Published Online: 2017-11-17

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Citation Information: Journal of Complementary and Integrative Medicine, Volume 15, Issue 2, 20150017, ISSN (Online) 1553-3840, DOI: https://doi.org/10.1515/jcim-2015-0017.

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