Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Journal of Complementary and Integrative Medicine

Editor-in-Chief: Lui, Edmund

Ed. by Ko, Robert / Leung, Kelvin Sze-Yin / Saunders, Paul / Suntres, PH. D., Zacharias

4 Issues per year


CiteScore 2017: 1.41

SCImago Journal Rank (SJR) 2017: 0.472
Source Normalized Impact per Paper (SNIP) 2017: 0.564

Online
ISSN
1553-3840
See all formats and pricing
More options …

The beneficial effects of pumpkin extract on atherogenic lipid, insulin resistance and oxidative stress status in high-fat diet-induced obese rats

Atefe Ghahremanloo / Reza Hajipour / Mina Hemmati
  • Corresponding author
  • Birjand CardioVascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Maryam Moossavi / Zabihullah Mohaqiq
Published Online: 2017-10-24 | DOI: https://doi.org/10.1515/jcim-2017-0051

Abstract

Background

Prevention and treatment of obesity is a way to reduce cardiovascular disease, diabetes and depression. Pumpkin as a favorable plant has different properties notably antioxidant, lipid-lowering and anti-diabetic potential. The aim of this study was to assess the anti-obesity effects of pumpkin in diet-induced obese rats.

Methods

Thirty adult male Wistar rats were randomly divided into five groups (n=6) of healthy control, dietary fatty control rats, and three experimental dietary fatty rats that received hydro-alcoholic extract of pumpkin once daily at doses 100 and 200 and 400 mg/kg, respectively. At the end of 6 weeks, lipid profile, atherogenicity, liver enzymes, and oxidative stress status were measured.

Results

Pumpkin in a dose-dependent manner dramatically decreased triglycerides and low-density lipoprotein, and liver enzymes while high-density lipoprotein was markedly increased in treated groups. Pumpkin also increased glutathione level in comparison with obese control group.

Conclusions

Pumpkin ameliorated oxidative stress and dyslipidemia in obese rats, leading to decrease cardiovascular disease risk in obesity.

Keywords: insulin resistance; obesity; pumpkin extract

References

  • [1]

    Hasani-Ranjbar S, Jouyandeh Z, Abdollahi M. A systematic review of anti-obesity medicinal plants-an update. J Diabetes Metab Disord. 2013;12:28.CrossrefGoogle Scholar

  • [2]

    Medjakovic S, Hobiger S, Ardjomand-Woelkart K, Bucar F, Jungbauer A. Pumpkin seed extract: Cell growth inhibition of hyperplastic and cancer cells, independent of steroid hormone receptors. Fitoterapia. 2016;110:150–56.CrossrefWeb of SciencePubMedGoogle Scholar

  • [3]

    Adams GG, Imran S, Wang S, Mohammad A, Kok S, Gray DA, et al. The hypoglycaemic effect of pumpkins as anti-diabetic and functional medicines. Food Res Int. 2011;44:862–67.CrossrefWeb of ScienceGoogle Scholar

  • [4]

    Hammer KA, Carson C, Riley T. Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol. 1999;86:985–90.CrossrefPubMedGoogle Scholar

  • [5]

    Sharma D, Rawat I, Goel HC. Anticancer and anti-inflammatory activities of some dietary cucurbits. Indian J Exp Biol. 2015;53:216–21.PubMedGoogle Scholar

  • [6]

    Hall JA, Seedarala S, Rice N, Kopel L, Halaweish F, Blagg BS. Cucurbitacin D Is a Disruptor of the HSP90 Chaperone Machinery. J Nat Prod. 2015;24;78:873–79.Web of ScienceGoogle Scholar

  • [7]

    Aghaie S, Nikzad H, Mahabadi JA, Taghizadeh M, Azami-Tameh A, Taherian A, et al. Protective effect of combined pumpkin seed and ginger extracts on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide. Anat Sci Int. 2016;91:382–90.PubMedCrossrefWeb of ScienceGoogle Scholar

  • [8]

    Xie J. Induced polarization effect of pumpkin protein on B16 cell. Fujian Med Univ Acta. 2004;38:394–95.Google Scholar

  • [9]

    Fissore E, Matkovic L, Wider E, Rojas A, Gerschenson L. Rheological properties of pectin-enriched products isolated from butternut (Cucurbita moschata Duch ex Poiret). LWT-food Sci Technol. 2009;42:1413–21.CrossrefWeb of ScienceGoogle Scholar

  • [10]

    Xanthopoulou MN, Nomikos T, Fragopoulou E, Antonopoulou S. Antioxidant and lipoxygenase inhibitory activities of pumpkin seed extracts. Food Res Int. 2009;42:641–46.CrossrefWeb of ScienceGoogle Scholar

  • [11]

    Peiretti PG, Meineri G, Gai F, Longato E, Amarowicz R. Antioxidative activities and phenolic compounds of pumpkin (Cucurbita pepo) seedsand amaranth (Amaranthus caudatus) grain extracts. Nat Prod Res. 2017;23:1–5.Google Scholar

  • [12]

    El-Mosallamy AE, Sleem AA, Abdel-Salam OM, Shaffie N, Kenawy SA. Antihypertensive and cardioprotective effects of pumpkin seed oil. J Med Food. 2012 Feb;15:180–89.CrossrefPubMedWeb of ScienceGoogle Scholar

  • [13]

    Barakat LA, Mahmoud RH. The antiatherogenic, renal protective and immunomodulatory effects of purslane, pumpkin and flax seeds on hypercholesterolemic rats. N Am J Med Sci. 2011;3:411–17.PubMedGoogle Scholar

  • [14]

    Xia T, Wang Q. Antihyperglycemic effect of Cucurbita ficifolia fruit extract in streptozotocin-induced diabetic rats. Fitoterapia. 2006;77:530–33.PubMedCrossrefGoogle Scholar

  • [15]

    Bayat A, Azizi-Soleiman F, Heidari-Beni M, Feizi A, Iraj B, Ghiasvand R, et al. Effect of cucurbita ficifolia and probiotic yogurt consumption on blood glucose, lipid profile, and inflammatory marker in type 2 diabetes. Int J Prev Med. 2016;7:30. DOI: CrossrefPubMedGoogle Scholar

  • [16]

    Abdel-Zaher AO, Salim SY, Assaf MH, Abdel-Hady RH. Antidiabetic activity and toxicity of Zizyphus spina-christi leaves. J Ethnopharmacol. 2005;101:129–38.CrossrefPubMedGoogle Scholar

  • [17]

    Yagi K. A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med. 1976;15:212–16.PubMedCrossrefGoogle Scholar

  • [18]

    Benzie IF, Strain J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem. 1996;239:70–76.CrossrefPubMedGoogle Scholar

  • [19]

    Lee MJ, Park JT, Han SH, Kim YL, Kim YS, Yang CW, et al. The atherogenic index of plasma and the risk of mortality in incident dialysis patients: Results from a nationwide prospective cohort in Korea. PLoS One. 2017;26;12:e0177499.Web of ScienceGoogle Scholar

  • [20]

    Mertz D. Atherosclerosis-index”(LDL/HDL): risk indicator in lipid metabolism disorders. Med Klin. 1980;75:159–61.PubMedGoogle Scholar

  • [21]

    Gayoso-Diz P, Otero-González A, Rodriguez-Alvarez MX, Gude F, García F, De Francisco A, et al. Insulin resistance (HOMA-IR) cut-off values and the metabolic syndrome in a general adult population: effect of gender and age: EPIRCE cross-sectional study. BMC Endocr Disord. 2013;13:47.CrossrefWeb of ScienceGoogle Scholar

  • [22]

    Bharti SK, Kumar A, Sharma NK, Prakash O, Jaiswal SK, Krishnan S, et al. Tocopherol from seeds of Cucurbita pepo against diabetes: validation by in vivo experiments supported by computational docking. J Formos Med Assoc. 2013;112:676–90.CrossrefWeb of SciencePubMedGoogle Scholar

  • [23]

    Hajati H, Hassanabadi A, Waldroup PW. Effects of dietary supplementation with pumpkin oil (Cucurbita pepo) on performance and blood fat of broiler chickens during finisher period. Am J Anim Vet Sci. 2011;6:40–44.CrossrefGoogle Scholar

  • [24]

    Fortis-Barrera Á, García-Macedo R, Almanza-Perez JC, Blancas-Flores G, Zamilpa-Alvarez A, Flores-Sáenz JL, et al. Cucurbita ficifolia (Cucurbitaceae) modulates inflammatory cytokines and IFN-γ in obese mice. Can J Physiol Pharmacol. 2017;95:170–77.Web of ScienceCrossrefPubMedGoogle Scholar

  • [25]

    Makni M, Fetoui H, Gargouri N, Garoui EM, Jaber H, Makni J, et al. Hypolipidemic and hepatoprotective effects of flax and pumpkin seed mixture rich in ω-3 and ω-6 fatty acids in hypercholesterolemic rats. Food Chem Toxicol. 2008;46:3714–20.CrossrefWeb of ScienceGoogle Scholar

  • [26]

    Choi H, Eo H, Park K, Jin M, Park E-J, Kim S-H, et al. A water-soluble extract from Cucurbita moschata shows anti-obesity effects by controlling lipid metabolism in a high fat diet-induced obesity mouse model. Biochem Biophys Res Commun. 2007;359:419–25.CrossrefWeb of ScienceGoogle Scholar

  • [27]

    Acosta-Patino J, Jimenez-Balderas E, Juarez-Oropeza M, Dıaz-Zagoya J. Hypoglycemic action of Cucurbita ficifolia on Type 2 diabetic patients with moderately high blood glucose levels. J Ethnopharmacol. 2001;77:99–101.CrossrefPubMedGoogle Scholar

  • [28]

    Farid HEA, EL-Sayed SM, Abozid MM. Pumpkin and Sunflower Seeds Attenuate Hyperglycemia and Protect Liver in Alloxan-Induced Diabetic Rats. Res J Pharmaceutical, Biol Chem Sci. 2015;6:1269.Google Scholar

  • [29]

    Mohamed RA, Ramadan RS, Ahmed LA. Effect of substituting pumpkin seed protein isolate for casein on serum liver enzymes, lipid profile and antioxidant enzymes in CCl4-intoxicated rats. Adv Biol Res. 2009;3:9–15.Google Scholar

  • [30]

    Yoshinari O, Sato H, Igarashi K. Anti-Diabetic Effects of Pumpkin and Its Components, Trigonelline and Nicotinic Acid, on Goto-Kakizaki Rats. Biosci Biotechnol Biochem. 2009;73:1033–41.PubMedCrossrefWeb of ScienceGoogle Scholar

About the article

Received: 2017-07-06

Accepted: 2017-07-27

Published Online: 2017-10-24


This investigation was supported by Grant No. 77.93 from the office of Vice Chancellor research, Birjand University of Medical Sciences.


Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Journal of Complementary and Integrative Medicine, Volume 15, Issue 2, 20170051, ISSN (Online) 1553-3840, DOI: https://doi.org/10.1515/jcim-2017-0051.

Export Citation

© 2018 Walter de Gruyter GmbH, Berlin/Boston.Get Permission

Comments (0)

Please log in or register to comment.
Log in