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Journal of Complementary and Integrative Medicine

Editor-in-Chief: Lui, Edmund

Ed. by Ko, Robert / Leung, Kelvin Sze-Yin / Saunders, Paul / Suntres, PH. D., Zacharias

CiteScore 2017: 1.41

SCImago Journal Rank (SJR) 2017: 0.472
Source Normalized Impact per Paper (SNIP) 2017: 0.564

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Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury

Mohammadreza Gholami
  • Department of Anatomical Science, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Abolfazl Abbaszadeh / Parastoo Baharvand / Afshin Hasanvand / Amin Hasanvand
  • Department of Pharmacology and Toxicology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anneh mohammad Gharravi
Published Online: 2018-05-23 | DOI: https://doi.org/10.1515/jcim-2016-0035



The aim of the present study was to survey the protective effect of pretreatment with Persian honey on amelioration of side effects of chemotherapy and ischemia/reperfusion induced testicular injury.

Materials and methods

Forty adult’s male wistar rats were divided into four groups of ischemia-reperfusion (IR), honey + ischemia-reperfusion (HIR), Busulfan (B) and Busulfan intraperitoneally+ honey (BH). The seminiferous tubules were rated for their modified spermatogenesis index (SI) by Johnsons score. Detection of single- and double-stranded DNA breaks at the early stages of apoptosis was performed using the in-situ cell death detection kit. Total serum concentration of Follicle-stimulating hormone (FSH) , Luteinizing hormone (LH) and testosterone was measured using ELISA. All data were expressed as mean ± SD and significance was set at p≤0.05.


Honey improved SI in the HIR and BH groups and serum levels of FSH and LH in the BH and HIR groups (p<0.001). Also, serum levels of testosterone were significantly higher in BH and HIR groups. But, apoptotic cells in IR and B groups significantly increased (p<0.001), while in HIR and BH groups, the number of apoptotic cells decreased and the positive cells of TUNEL (TdT-mediated dUTP-X nick end labelling) staining were detected in spermatocytes and spermatid.


Pretreatment with honey protect testis against chemotherapy and testicular IR injury, increase FSH and LH and testosterone and decrease the cellular damage and apoptosis. Honey can decrease the side effects of chemotherapy on reproductive system and prevent sterility.

Keywords: busulfan; honey; ischemia/reperfusion; spermatogenesis; testis


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About the article

Received: 2016-04-24

Accepted: 2018-03-30

Published Online: 2018-05-23

Author contributions: The authors contributed equally in the study concept and design, acquisition of data, statistical analysis, interpretation of data, and drafting of the manuscript.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Citation Information: Journal of Complementary and Integrative Medicine, Volume 15, Issue 4, 20160035, ISSN (Online) 1553-3840, DOI: https://doi.org/10.1515/jcim-2016-0035.

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