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Journal of Complementary and Integrative Medicine

Editor-in-Chief: Lui, Edmund

Ed. by Ko, Robert / Leung, Kelvin Sze-Yin / Saunders, Paul / Suntres, PH. D., Zacharias


CiteScore 2017: 1.41

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1553-3840
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Nephroprotective properties of the methanol stem extract of Abrus precatorius on gentamicin-induced renal damage in rats

Olufunke Olubunmi Falayi
  • Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria
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/ Ademola Adetokunbo Oyagbemi
  • Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria
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/ Temidayo Olutayo Omobowale
  • Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria
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/ Emmanuel Abiodun Ayodele / Aduragbenro Deborah Adedapo
  • Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria
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/ Momoh Audu Yakubu
  • Department of Environmental and Interdisciplinary Sciences, College of Science, Technology and Engineering, Texas Southern University, 3100 Cleburne Avenue, Houston, TX 77004, USA
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/ Adeolu Alex Adedapo
  • Corresponding author
  • Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria
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Published Online: 2018-10-26 | DOI: https://doi.org/10.1515/jcim-2017-0176

Abstract

Background

The use of plants for the treatment and prevention of diseases in man and his animals has led to a renewed scientific interest in the use of medicinal plants for therapeutic purposes. The nephroprotective properties of methanol stem bark extract of Abrus precatorius against gentamicin-induced renal damage in rats was evaluated in this study.

Methods

Thirty male Wistar rats were divided into five equal groups. Group A was the negative control group while B was the positive control group which received gentamicin 100 mg/kg intra-peritoneally for 6 days. Group C were pretreated with 100 mg/kg extract for the 3 days and then concurrently with gentamicin 100 mg/kg for 3 days and group D were pretreated with 200 mg/kg extract for 3 days and then concurrently with gentamicin 100 mg/kg for 3 days. Group E received gentamicin intraperitoneally for 6 days followed by administration of 200 mg/kg of the extract for 3 days. Blood samples, kidneys and kidney homogenates were collected for haematological, biochemical, histopathological and immunohistochemical analysis.

Results

The results showed that no significant haematological changes were noted. The groups treated with extract exhibited significant increase in body weight gain. While group B significantly exhibited focal areas of inflammation, fatty degeneration, congestion of vessels, tubular necrosis and glomerular atrophy, the lesions were mild with the treated groups. Treated groups exhibited a dose dependent significant decrease in serum creatinine, urea, XO, NO and Myeloperoxidase, AOPP, Protein carbonyl, H2O2 generated and MDA levels when compared with group B. There were significant dose dependent improvements in SOD, GST, GSH, Protein thiol, and non-protein thiol levels in the treated groups when compared with group B. In immunohistochemistry, Group B exhibited over expression of CRP and NF-κB levels, and marked reduction in expression of Bcl-2 while the reverse was seen in the groups treated with methanol extracts of Abrus precatorius.

Conclusion

The methanol extract of Abrus precatorius plays a vital role against gentamicin induced renal damage by reducing levels of renal markers of oxidative stress, inflammation and apoptosis, enhancing enzymatic and non enzymatic renal antioxidant system, alongside an increase in Bcl-2 and a decrease in NF-κB and CRP expressions.

Keywords: Abrus precatorius; anti-oxidant; gentamicin; immunohistochemistry; nephroprotection

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About the article

Correction note: Correction added after ahead-of-print publication on October 26, 2018: The first name and last name of the authors has been changed to reflect the correct order.


Received: 2017-12-20

Accepted: 2018-09-13

Published Online: 2018-10-26


Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: National Research Foundation of the Tertiary Education Trust Fund (TETFUND), Abuja, Nigeria (TETFUND/DESS/NRF/UI IBADAN/STI/VOL/I/B2.20.11)

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Journal of Complementary and Integrative Medicine, 20170176, ISSN (Online) 1553-3840, DOI: https://doi.org/10.1515/jcim-2017-0176.

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