Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Journal of Complementary and Integrative Medicine

Editor-in-Chief: Lui, Edmund

Ed. by Ko, Robert / Leung, Kelvin Sze-Yin / Saunders, Paul / Suntres, PH. D., Zacharias


CiteScore 2017: 1.41

SCImago Journal Rank (SJR) 2017: 0.472
Source Normalized Impact per Paper (SNIP) 2017: 0.564

Online
ISSN
1553-3840
See all formats and pricing
More options …

Metabolomic fingerprint of Mentha rotundifolia L. Leaf tissues promotes this species as a potential candidate for sustainable production of biologically active molecules

Leila Riahi
  • Corresponding author
  • Laboratory of Biotechnology and Bio-Geo Resources Valorization BVBGR-LR11ES31, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, 2020, Sidi Thabet, Ariana, Tunisia
  • Laboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj Cedria, B.P. 901, Hammam-Lif 2050, Tunisia
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hanen Chakroun
  • Biology Department, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Imen Klay
  • Laboratory of Biotechnology and Bio-Geo Resources Valorization BVBGR-LR11ES31, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, 2020, Sidi Thabet, Ariana, Tunisia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ahmed Slaheddine Masmoudi
  • Laboratory of Biotechnology and Bio-Geo Resources Valorization BVBGR-LR11ES31, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, 2020, Sidi Thabet, Ariana, Tunisia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ameur Cherif
  • Laboratory of Biotechnology and Bio-Geo Resources Valorization BVBGR-LR11ES31, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, 2020, Sidi Thabet, Ariana, Tunisia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Nejia Zoghlami
  • Laboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj Cedria, B.P. 901, Hammam-Lif 2050, Tunisia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2018-10-17 | DOI: https://doi.org/10.1515/jcim-2018-0048

Abstract

Background

The Mentha rotundifolia L. (Lamiaceae family), is a medicinal herb used since the ancient times as an antiseptic, analgesic and anti-inflammatory agent. In the present work, metabolomic profiling of two Mentha rotundifolia L. ecotypes leaf tissues spontaneously growing in the North of Tunisia was achieved.

Methods

Phenolic contents (TPC, TFC and TTC) were assessed using colorimetric methods. Metabolomic profiling of leaf tissues extracts was assessed based on Gas Chromatography-Mass Spectrometry (GC/MS) analysis. The antioxidant ability of M. rotundifolia extracts was achieved based on two test systems namely DPPH and FRAP assays. Antimicrobial activity against a set of Gram negative and Gram positive bacteria was estimated by measuring ID, MIC and MBC values.

Results

Fifty metabolites were identified as belonging mainly to phenolics, fatty acids, terpenes, steroids and aldehydes classes with qualitative and quantitative variability. Most of the identified compounds are reputed bioactive with potent antioxidant, antimicrobial and anti-inflammatory among others effects. To confirm these findings common in vitro biological activities were achieved. The investigated extracts showed significant antioxidant abilities based on both 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Ferric reducing antioxidant potential (FRAP) assays. Furthermore, the extracts revealed promising antimicrobial ability against tested Gram+ and Gram- bacterial strains (ID: 12.5–14.5 mm, MIC: 3.125–25 (µg/mL), MBC: 6.25–100 (µg/mL)).

Conclusions

Based on our findings Mentha rotundifolia L. leaves extracts present a potential source of natural antioxidants and diverse bioactive compounds which could be used in green pharmacy, food preservation, alternative medicine and natural therapies.

Keywords: alternative medicine; bioactivity; GC-MS; Mentha rotundifolia L.; metabolome

References

  • [1]

    Lu JM, Lin PH, Yao Q, Chen C. Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems. J Cell Mol Med. 2010;14:840–60.PubMedWeb of ScienceCrossrefGoogle Scholar

  • [2]

    Chung HY, Cesari M, Anton S, Marzetti E, Giovannini S, Seo AY, et al. Molecular inflammation: underpinnings of aging and age-related diseases. Ageing Res Rev. 2009;8:18–30.CrossrefWeb of SciencePubMedGoogle Scholar

  • [3]

    Edwards MR, Bartlett NW, Clarke D, Birrell M, Belvisi M, Johnston SL. Targeting the NF-κB pathway in asthma and chronic obstructive pulmonary disease. Pharmacol Ther. 2009;121:1–13.CrossrefPubMedWeb of ScienceGoogle Scholar

  • [4]

    Read SA, Douglas MW. Virus induced inflammation and cancer development. Cancer Lett. 2014;345:174–81.CrossrefWeb of SciencePubMedGoogle Scholar

  • [5]

    Tatiya AU, Saluja AK, Kalaskar MG, Surana SJ, Patil PH. Evaluation of analgesic and anti-inflammatory activity of Bridelia retusa (Spreng) bark. J Tradit Complement Med. 2017;7:441–51.CrossrefPubMedGoogle Scholar

  • [6]

    Prestinaci F, Pezzotti P, Pantosti A. Antimicrobial resistance: a global multifaceted phenomenon. Pathog Glob Health. 2015;109:309–18.PubMedWeb of ScienceCrossrefGoogle Scholar

  • [7]

    Conforti F, Sosa S, Marrelli M, Menichini F, Statti GA, Uzunov D, et al. In vivo anti-inflammatory and in vitro antioxidant activities of Mediterranean dietary plants. J Ethnopharmacol. 2008;116:144–51.PubMedWeb of ScienceCrossrefGoogle Scholar

  • [8]

    Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pac J Trop Biomed. 2012;2:S178–S180.CrossrefGoogle Scholar

  • [9]

    Kar D, Kuanar A, Pattanaik PK. Antimicrobial activities of different parts of calotropis gigantea: a naturally occurring prophylactic medicinal shrub. Iran J Sci Technol Trans Sci. 2018;42:1057–62.Web of ScienceCrossrefGoogle Scholar

  • [10]

    Kumar K, Gupta SM, Arya MC, Nasim M. In vitro antimicrobial and antioxidant activity of camelina seed extracts as potential source of bioactive compounds. Proc Natl Acad Sci India Sect B Biol Sci. 2017;87:521–26.CrossrefGoogle Scholar

  • [11]

    Abat JK, Kumar S, Mohanty A. Ethnomedicinal, phytochemical and ethnopharmacological aspects of four medicinal plants of Malvaceae used in Indian traditional medicines: a review. Medicines. 2017;4:75.CrossrefGoogle Scholar

  • [12]

    Brahmi F, Hauchard D, Guendouze N, Madani K, Kiendrebeogo M, Kamagaju L, et al. Phenolic composition, in vitro antioxidant effects and tyrosinaseinhibitory activity of three Algerian Mentha species: M. spicata (L.), M.pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae). Ind Crops Prod. 2015;74:722–30.CrossrefGoogle Scholar

  • [13]

    Derwich E, Benziane Z, Boukir A, Gc-Ms BL. Analysis of the leaf essential oil of Mentha rotundifolia, a traditional herbal medicine in Morocco. Chem Bull Politech Univ Timisoara. 2009;2:85–88.Google Scholar

  • [14]

    Salhi S, Fadli M, Zidane L, Douira A. Etudes floristique et ethnobotanique des plantes médicinales de la ville de Kénitra (Maroc). Lazaroa. 2010;31:133–46.CrossrefGoogle Scholar

  • [15]

    Brahmi F, Madani K, Stévigny C, Chibane M, Duez P. Algerian mintspecies: HPTLC quantitative determination of rosmarinic acid and in vitro inhibitory effects on linoleic acid peroxidation. J Coast Life Med. 2014;2:986–92.Google Scholar

  • [16]

    Jdaidi N, Hasnaoui B. Étude floristique et ethnobotanique des plantes médicinales au nord-ouest de la Tunisie : cas de la communauté d’Ouled Sedra. J Adv Res Sci Tech. 2016;3:281–91.Google Scholar

  • [17]

    Osorio S, Alba R, Nikoloski Z, Kochevenko A, Fernie AR, Giovannoni JJ. Integrative comparative analyses of transcript and metabolite profiles from pepper and tomato ripening and development stages uncovers species-specific patterns of network regulatory behavior[W][OA]. Plant Physiol. 2012;159:1713–29.Web of ScienceCrossrefGoogle Scholar

  • [18]

    Lavergne FD, Broeckling CD, Cockrell DM, Haley SD, Peairs FB, Jahn CE, et al. GC-MS metabolomics to evaluate the composition of plant cuticular waxes for four Triticum aestivum cultivars. Int J Mol Sci. 2018;19:249.CrossrefWeb of ScienceGoogle Scholar

  • [19]

    Singleton VL, Rosi JA. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am J Oenol Vitic. 1965;16:144–58.Google Scholar

  • [20]

    Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocher P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006;97:654–60.CrossrefGoogle Scholar

  • [21]

    Sun B, Ricardo-da-Silva JM, Spranger I. Critical factors of vanillin assay for catechins and proanthocyanidins. J Agric Food Chem. 1998;46:4267–74.CrossrefGoogle Scholar

  • [22]

    Belhadj F, Somrani I, Aissaoui N, Messaoud C, Boussaid M, Marzouki MN. Bioactive compounds contents, antioxidant and antimicrobial activities during ripening of Prunus persica L. varieties from the North West of Tunisia. Food Chem. 2016;204:29–36.PubMedCrossrefWeb of ScienceGoogle Scholar

  • [23]

    Messaoud C, Laabidi A, Boussaid M. Myrtus communis L. Infusions: the effect of infusion time on phytochemical composition, antioxidant, and antimicrobial activities. J Food Sci. 2012;77:C941–C947.Web of ScienceGoogle Scholar

  • [24]

    Blois MS Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199–200.CrossrefGoogle Scholar

  • [25]

    Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem. 1996;239:70–76.PubMedCrossrefGoogle Scholar

  • [26]

    National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial susceptibility testing. Twelfth informational supplement. M100–S12. NCCLS, Wayne, PA; 2002.Google Scholar

  • [27]

    Riahi L, Ghazghazi H, Ayari B, Aouadhi C, Klay I, Chograni H, et al. Effect of environmental conditions on chemical polymorphism and biological activities among Artemisia absinthium L. essential oil provenances grown in Tunisia. Ind Crops Prod. 2015;66:96–102.Web of ScienceCrossrefGoogle Scholar

  • [28]

    SAS. SAS User’s Guide: SAS Stat, SAS Basic (Version 6). Cary, NC: SAS Institute Inc., 1990.Google Scholar

  • [29]

    Barchan A, Bakkali M, Arakrak A, Pagán R, Laglaoui A. The effects of solvents polarity on the phenolic contents and antioxidant activity of three Mentha species extracts. Int J Curr Microbiol App Sci. 2014;3:399–412.Google Scholar

  • [30]

    Brahmi F, Dahmoune F, Kadri N, Chibane M, Dairi S, Remini H, et al. Antioxidant capacity and phenolic content of two Algerian Mentha species M. rotundifolia (L.) Huds, M. pulegium L., extracted with different solvents. J Compl Integr Med. 2017;14:1–9.Google Scholar

  • [31]

    Dhawan D, Gupta J. Comparison of different solvents for phytochemical extraction potential from Datura metel plant leaves. Int J Biol Chem. 2017;11:17–22.Google Scholar

  • [32]

    Mediani A, Abas F, Khatib A, Tan CP, Ismail IS, Shaari K, et al. Relationship between metabolites composition and biological activities of Phyllanthus niruri extracts prepared by different drying methods and solvents extraction. Plant Foods Hum Nutr. 2015;70:184–92.PubMedCrossrefWeb of ScienceGoogle Scholar

  • [33]

    Safdar MN, Kausar T, Jabbar S, Mumtaz A, Ahad K, Saddozai AA. Extraction and quantification of polyphenols from kinnow (Citrus reticulate L.) peel using ultrasound and maceration techniques. J Food Drug Anal. 2017;25:488–500.Web of SciencePubMedCrossrefGoogle Scholar

  • [34]

    Elansary HO, Norrie J, Ali HM, Salem MZM, Mahmoud EA, Yessoufou K. Enhancement of Calibrachoa growth, secondary metabolites and bioactivity using seaweed extracts. BMC Compl Alt Med. 2016;16:341.CrossrefGoogle Scholar

  • [35]

    Singh B, Sahu PM, Sharma RA. Flavonoids from Heliotropium subulatum exudates and their evaluation for antioxidant, antineoplastic and cytotoxic activities II. Cytotechnology. 2017;69:103–15.CrossrefGoogle Scholar

  • [36]

    Pinent M, Castell A, Baiges I, Montagut G, Arola L, Ardévol A. Bioactivity of flavonoids on insulin-secreting cells. Comp Rev Food Sci Food Saf. 2008;7:299–308.CrossrefGoogle Scholar

  • [37]

    Hossain MA, Al-Hdhrami SS, Mohammed Weli A, Al-Riyami Q, Nasser Al-Sabahi J. Isolation, fractionation and identification of chemical constituents from the leaves crude extracts of Mentha piperita L. grown in Sultanate of Oman. Asian Pac J Trop Biomed. 2014;4:S368–S372.CrossrefGoogle Scholar

  • [38]

    Sagadevan P, Suresh SN, Kumar SR, Revathy Rajan AR. Studies on Phytochemical composition, antibacterial and antioxidant potential of methanolic leaf extract of Mentha arvensis. Int J Biosci Nanosci. 2014;1:22–27.Google Scholar

  • [39]

    Kim KB, Nam YA, Kim HS, Hayes AW, Lee BM. α-Linolenic acid: nutraceutical, pharmacological and toxicological evaluation. Food Chem Toxicol. 2014;70:163–78.PubMedWeb of ScienceCrossrefGoogle Scholar

  • [40]

    Rajalakshmi K, Mohan VR. GC-MS analysis of bioactive components of Myxopyrum serratulum A.W. Hill (Oleaceae). Int J Pharm Sci Rev Res. 2016;38:30–35.Google Scholar

  • [41]

    Benabdallah A, Rahmoune C, Boumendjel M, Aissi O, Messaoud C. Total phenolic content and antioxidant activity of six wild Mentha species (Lamiaceae) from northeast of Algeria. Asian Pac J Trop Biomed. 2016;6:760–66.Web of ScienceCrossrefGoogle Scholar

  • [42]

    Nickavar B, Alinaghi A, Kamalinejad M. Evaluation of the antioxidant properties of five Mentha species. Iran J Pharm Res. 2008;7:203–09.Google Scholar

  • [43]

    Gulluce M, Sahin F, Sokmen M, Ozer H, Daferera D, Sokmen A, et al. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food Chem. 2007;103:1449–56.Web of ScienceCrossrefGoogle Scholar

  • [44]

    Biswas NN, Saha S, Ali MK. Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. Asian Pac J Trop Biomed. 2014;4:792–97.CrossrefGoogle Scholar

  • [45]

    Naz R, Ayub H, Nawaz S, ZUl I, Yasmin T, Bano A, et al. Antimicrobial activity, toxicity and anti-inflammatory potential of methanolic extracts of four ethnomedicinal plant species from Punjab Pakistan. BMC Compl Alt Med. 2017;17:302.CrossrefGoogle Scholar

  • [46]

    Riahi L, Chograni H, Elferchichi M, Zaouali Y, Zoghlami N, Mliki A. Variations in Tunisian wormwood essential oil profiles and phenolic contents between leaves and flowers and their effects on antioxidant activities. Ind Crops Prod. 2013;46:290–96.CrossrefWeb of ScienceGoogle Scholar

  • [47]

    Sanhueza L, Tello M, Vivanco M, Mendoza L, Wilkens M. Relation between antibacterial activity against food transmitted pathogens and total phenolic compounds in grape pomace extracts from Cabernet Sauvignon and Syrah varieties. Adv Microbiol. 2014;4:225–32.CrossrefGoogle Scholar

  • [48]

    Behbahani B, Shahidi F, Yazdi FT, Mortazavi SA, Mohebbi M. Antioxidant activity and antimicrobial effect of tarragon (Artemisia dracunculus) extract and chemical composition of its essential oil. Food Meas. 2017;11:847–63.CrossrefWeb of ScienceGoogle Scholar

About the article

Received: 2018-04-08

Accepted: 2018-07-17

Published Online: 2018-10-17


Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Journal of Complementary and Integrative Medicine, 20180048, ISSN (Online) 1553-3840, DOI: https://doi.org/10.1515/jcim-2018-0048.

Export Citation

© 2018 Walter de Gruyter GmbH, Berlin/Boston.Get Permission

Comments (0)

Please log in or register to comment.
Log in