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Journal of Complementary and Integrative Medicine

Editor-in-Chief: Lui, Edmund

Ed. by Ko, Robert / Leung, Kelvin Sze-Yin / Saunders, Paul / Suntres, PH. D., Zacharias

CiteScore 2017: 1.41

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Metabolomic fingerprint of Mentha rotundifolia L. Leaf tissues promotes this species as a potential candidate for sustainable production of biologically active molecules

Leila Riahi
  • Corresponding author
  • Laboratory of Biotechnology and Bio-Geo Resources Valorization BVBGR-LR11ES31, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, 2020, Sidi Thabet, Ariana, Tunisia
  • Laboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj Cedria, B.P. 901, Hammam-Lif 2050, Tunisia
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/ Hanen Chakroun
  • Biology Department, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia
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/ Imen Klay
  • Laboratory of Biotechnology and Bio-Geo Resources Valorization BVBGR-LR11ES31, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, 2020, Sidi Thabet, Ariana, Tunisia
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/ Ahmed Slaheddine Masmoudi
  • Laboratory of Biotechnology and Bio-Geo Resources Valorization BVBGR-LR11ES31, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, 2020, Sidi Thabet, Ariana, Tunisia
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/ Ameur Cherif
  • Laboratory of Biotechnology and Bio-Geo Resources Valorization BVBGR-LR11ES31, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, 2020, Sidi Thabet, Ariana, Tunisia
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/ Nejia Zoghlami
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Published Online: 2018-10-17 | DOI: https://doi.org/10.1515/jcim-2018-0048



The Mentha rotundifolia L. (Lamiaceae family), is a medicinal herb used since the ancient times as an antiseptic, analgesic and anti-inflammatory agent. In the present work, metabolomic profiling of two Mentha rotundifolia L. ecotypes leaf tissues spontaneously growing in the North of Tunisia was achieved.


Phenolic contents (TPC, TFC and TTC) were assessed using colorimetric methods. Metabolomic profiling of leaf tissues extracts was assessed based on Gas Chromatography-Mass Spectrometry (GC/MS) analysis. The antioxidant ability of M. rotundifolia extracts was achieved based on two test systems namely DPPH and FRAP assays. Antimicrobial activity against a set of Gram negative and Gram positive bacteria was estimated by measuring ID, MIC and MBC values.


Fifty metabolites were identified as belonging mainly to phenolics, fatty acids, terpenes, steroids and aldehydes classes with qualitative and quantitative variability. Most of the identified compounds are reputed bioactive with potent antioxidant, antimicrobial and anti-inflammatory among others effects. To confirm these findings common in vitro biological activities were achieved. The investigated extracts showed significant antioxidant abilities based on both 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Ferric reducing antioxidant potential (FRAP) assays. Furthermore, the extracts revealed promising antimicrobial ability against tested Gram+ and Gram- bacterial strains (ID: 12.5–14.5 mm, MIC: 3.125–25 (µg/mL), MBC: 6.25–100 (µg/mL)).


Based on our findings Mentha rotundifolia L. leaves extracts present a potential source of natural antioxidants and diverse bioactive compounds which could be used in green pharmacy, food preservation, alternative medicine and natural therapies.

Keywords: alternative medicine; bioactivity; GC-MS; Mentha rotundifolia L.; metabolome


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About the article

Received: 2018-04-08

Accepted: 2018-07-17

Published Online: 2018-10-17

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Citation Information: Journal of Complementary and Integrative Medicine, 20180048, ISSN (Online) 1553-3840, DOI: https://doi.org/10.1515/jcim-2018-0048.

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