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Journal of Horticultural Research

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Low temperature seed germination of cucumber: genetic basis of the tolerance trait

Urszula Kłosińska / Elżbieta U. Kozik / Marcin Nowicki / Todd C. Wehner
Published Online: 2014-02-27 | DOI: https://doi.org/10.2478/johr-2013-0031


Cucumber (Cucumis sativus L.) germinates in an optimal temperature ranging from 24 to 28 °C. In order to develop cultivars with low temperature germination ability, knowledge regarding its genetic basis is needed. In our earlier study, we identified the accession PI 390953 as chilling tolerant and a good cold germinator. The objective of our present study was to compare cold germinability of cold tolerant breeding line B 5669 with PI 390953, and to measure the inheritance of this trait. At 13 °C, both tested cultigens (B 5669, PI 390953) showed the highest germinability and we found no significant differences between them regarding the rate of germination, days to germination (DTG), or germination index (GI). We also observed differences in the germination ability at 13 °C among seven hybrid populations of cucumber, derived from the cross between good cold germinator B 5669 (P1) and B 6115 (P2) lacking cold-germination ability. The fastest low temperature germination and the highest low temperature germination percentages were observed in B 5669 (P1) with germination of 78 and 100% on the 6th and 10th day of the test, respectively. In addition, the cultigen B 5669 exhibited the fastest germination, reaching on average of DTG = 5.7. B 6115 (P2) and BC1P2 proved unable to germinate at 13 °C even within 21 days. The seed germinability of F2 population fits a three-recessive gene model. Cucumber cultigens B 5669, PI 390953, and PI 246903 showed low temperature tolerance, but of them B 5669 may become the most desirable to breeders since it exhibits cold germinability combined with good fruit quality traits.

Keywords : Chilling; inheritance; cold tolerance; Cucumis sativus; genetic factors


  • Cabrera R.M., Saltveit Jr. M.E., Owens K. 1992. Cucumber cultivars differ in their response to chilling temperatures. J. Amer. Soc. Hort. Sci. 117: 802-807.Google Scholar

  • Chung S.M., Staub J.E., Fazio G. 2003. Inheritance of chilling injury: a maternally inherited trait in cucumber. J. Amer. Soc. Hort. Sci. 128: 526-530.Google Scholar

  • De Vos D.A., Hill R.R. Jr., Hepler R.W., Garwood D.L. 1981. Inheritance of low temperature sprouting ability in F1 tomato crosses. J. Amer. Soc. Hort. Sci. 106: 352-355.Google Scholar

  • Foolad M.R., Lin G.Y. 1998. Genetic analysis of low-temperature tolerance during germination in tomato, Lycopersicon esculentum Mill. Plant Breeding 117: 171-176. DOI:10.1111/j.1439-0523.1998.tb01473.x.CrossrefGoogle Scholar

  • Foolad M.R., Lin G.Y. 2001. Relationship between cold tolerance during seed germination and vegetative growth in tomato: analysis of response and correlated response to selection. J. Amer. Soc. Hort. Sci. 126: 216-220.Google Scholar

  • Hutton M.G., Loy B.J. 1992. Inheritance of cold germinability in muskmelon. HortScience 27: 826-829.Google Scholar

  • Kozik E.U., Kłosińska U., Wehner T.C. 2007. New sources of chilling resistance in cucumber. In: Nowaczyk P. (Ed.), Spontaneous and induced variation for the genetic improvement of horticultural crops. University Press, University of Technology and Life Sciences, Bydgoszcz, Poland, pp. 227-232.Google Scholar

  • Kozik E.U., Wehner T.C. 2008. A single dominant gene Ch for chilling resistance in cucumber seedlings. J. Amer. Soc. Hort. Sci. 133: 225-227.Google Scholar

  • Kozik E.U., Kłosińska U., Wehner T.C. 2010. Progress in the development of cucumber breeding lines with low-temperature resistance. In: Thies J.A., Kousik S., Levi A. (Eds.), Cucurbitaceae 2010 Proc. Charleston, SC, US, pp. 46-49.Google Scholar

  • Kozik E.U., Kłosińska U., Wehner T.C. 2012. Inheritance of low temperature seed germination ability in cucumber. In: Proc. of 10th Eucarpia International Meeting on Genetics and Breeding Cucurbitaceae 2012, Antalya, Turkey, pp. 575-578.Google Scholar

  • Li J., Cui H., Zhang M. 1998. The relationship between low-temperature germination and chilling tolerance in cucumber. Cucurbit Genet. Coop. Rpt. 21: 11-13.Google Scholar

  • Lower R.L. 1975. Measurement and selection for cold tolerance in cucumber. Pickle Pak. Sci. 4: 8-11.Google Scholar

  • Nerson H., Staub J.E, 1989. Low temperature germination in muskmelon is dominant. Cucurbit Genet. Coop. Rpt. 12: 50-51.Google Scholar

  • Nienhuis J., Lower R.L. 1981. An estimate of the heritability of low temperature and seed germination in cucumber. Cucurbit Genet. Coop. Rpt. 4: 12-13.Google Scholar

  • Ng T.J., Tigchelaar E.C. 1973. Inheritance of low temperature seed sprouting in tomato. J. Amer. Soc. Hort. Sci. 98: 314-316.Google Scholar

  • Røeggen O. 1987. Variation in minimum germination temperature for cultivars of bean (Phaseolus vulgaris L.) cucumber (Cucumis sativus L.) and tomato (Lycopersicum esculentum Mill.). Sci. Hortic. 33: 7-65. DOI: 10.1016/0304-4238(87)90032-X.CrossrefGoogle Scholar

  • Smeets L., Wehner T.C. 1997. Environmental effects on genetic variation of chilling resistance in cucumber. Euphytica 97: 217-225.CrossrefGoogle Scholar

  • Smith P.G., Millet A.H. 1964. Germinating and sprouting responses of tomato at low temperatures. J. Amer. Soc. Hort. Sci. 84: 480-484.Google Scholar

  • Staub J.E., Wehner T.C. 1996. Temperature stress. In: Zitter T.A., Hopkins D.L., Thomas C.E. (Eds.), Compendium of cucurbit diseases. APS Press, St. Paul, MN, USA, pp 66-67.Google Scholar

  • Wehner T.C. 1981. Screening for low-temperature germination ability in cucumber. HortScience 16: 399. (abstr.).Google Scholar

  • Wehner T.C. 1982. Genetic variation for low-temperature germination ability in cucumber. Cucurbit Genet. Coop. Rpt. 5: 16-17.Google Scholar

  • Wehner T.C. 1984. Estimates of heritabilities and variance components for low-temperature germination ability in cucumber. J. Amer. Soc. Hort. Sci. 109: 664-667.Google Scholar

About the article

Published Online: 2014-02-27

Published in Print: 2013-12-01

Citation Information: Journal of Horticultural Research, ISSN (Online) 2300-5009, DOI: https://doi.org/10.2478/johr-2013-0031.

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