Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Journal of Medical Biochemistry

The Journal of Society of Medical Biochemists of Serbia

4 Issues per year


IMPACT FACTOR 2016: 1.148

CiteScore 2016: 0.84

SCImago Journal Rank (SJR) 2016: 0.279
Source Normalized Impact per Paper (SNIP) 2016: 0.488

Open Access
Online
ISSN
1452-8266
See all formats and pricing
More options …
Volume 29, Issue 1 (Jan 2010)

Issues

The Change of Ghrelin Levels in Intestinal Parasitic Infections

Ahmet Erensoy / Suleyman Aydin
  • Department of Biochemistry and Clinical Biochemistry, School of Medicine, Firat University, Elazig, Turkey
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Neslihan Kelestimur / Sevda Kirbag / Salih Kuk
Published Online: 2010-01-06 | DOI: https://doi.org/10.2478/v10011-010-0004-0

The Change of Ghrelin Levels in Intestinal Parasitic Infections

The aim of this work was to examine the relationship between active (acylated ghrelin) and inactive (desacylated ghrelin) ghrelin in the serum and other serum parameters in intestinal parasitic infections and healthy controls. Conventional microscopic methods (saline and iodine solutions, trichrome stain) were used to identify intestinal parasites in stool samples of 29 subjects attending Firat University Hospital. Serum parameters were assessed in a single measurement of serum from 29 parasite subjects, and in 18 healthy controls. Serum acylated ghrelin and desacylated ghrelin levels were measured using a commercial radioimmunoassay (RIA) kit. Paraoxonase and arylesterase were measured by using a spectrophotometer at 405 nm and 270 nm, respectively. Serum concentrations of acylated ghrelin and desacylated ghrelin were more markedly decreased in helminth bearing patients than the control group. Glucose, cholesterol and triglyceride levels were higher in intestinal parasitic infections than in controls. Furthermore, there were no correlations between ghrelin levels and BMI. These results indicate that low ghrelin and PON1/AE level may be important for appetite monitoring in intestinal parasitic infections.

Promene Nivoa Grelina kod Crevnih Parazitskih Infekcija

Cilj studije bio je da se ispita odnos između aktivnog (aciliranog) i neaktivnog (deaciliranog) grelina u serumu i drugih serumskih parametara kod crevnih parazitskih infekcija i zdravih kontrola. Uobičajene mikroskopske metode (slani rastvor i rastvor joda, trihromatski otisak) korišćene su za identifikaciju crevnih parazita u uzorcima stolice 29 pacijenata Univerzitetske bolnice Firat. Serumski parametri određeni su u jednokratnom merenju seruma 29 pacijenata s parazitima i 18 zdravih kontrolnih subjekata. Nivoi aciliranog grelina i deaciliranog grelina u serumu izmereni su metodom radioimunoeseja. Paraoksonaza i arilesteraza su merene spektrofotometrijski na 405 nm i 270 nm. Koncentracije aciliranog i deaciliranog grelina u serumu bile su upadljivo niže kod pacijenata sa parazitima nego u kontrolnoj grupi. Nivoi glukoze, holesterola i triglicerida bili su viši kod crevnih parazitskih infekcija nego kod kontrola. Štaviše, nije bilo korelacija između nivoa grelina i indeksa telesne mase. Rezultati ukazuju na to da bi niski nivoi grelina i PON1/AE mogli biti važni za praćenje apetita kod crevnih parazitskih infekcija.

Keywords: serum; active/inactive ghrelin; paraoxonase; arylesterase; parasite

Keywords: serum; aktivni/neaktivni grelin; paraoksonaza; arilesteraza; paraziti

  • Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 1999; 402: 656-60.Google Scholar

  • Hosoda H, Kojima M, Matsuo H, Kangawa K. Ghrelin and des-acyl ghrelin: two major forms of rat ghrelin peptide in gastrointestinal tissue. Biochem Biophys Res Commun 2000; 279: 909-13.Google Scholar

  • Hosoda H, Kojima M, Mizushima T, Shimizu S, Kangawa K. Structural divergence of human ghrelin. Identification of multiple ghrelin-derived molecules produced by post-translational processing. J Biol Chem 2003; 278: 64-70.Google Scholar

  • Carlini VP, Monzon ME, Varas MM, Cragnolini AB, Schiöth HB, Scimonelli TN, et al. Ghrelin increases anxiety-like behavior and memory retention in rats. Biochem Biophys Res Commun 2002; 299: 739-43.Google Scholar

  • Fujino K, Inui A, Asakawa A, Kihara N, Fujimura M, Fujimiya M. Ghrelin induces fasted motor activity of the gastrointestinal tract in conscious fed rats. J Physiol 2003; 550: 227-40.Google Scholar

  • Inui A. Ghrelin: an orexigenic and somatotrophic signal from the stomach. Nat Rev Neurosci 2001; 2: 551-60.CrossrefPubMedGoogle Scholar

  • Kojima M, Kangawa K. Ghrelin, an orexigenic signaling molecule from the gastrointestinal tract. Curr Opin Pharmacol 2002; 2: 665-8.PubMedCrossrefGoogle Scholar

  • Nagaya N, Kangawa K. Ghrelin improves left ventricular dysfunction and cardiac cachexia in heart failure. Curr Opin Pharmacol 2003; 3: 146-51.CrossrefPubMedGoogle Scholar

  • Yoshimoto A, Mori K, Sugawara A, Mukoyama M, Yahata K, Suganami T, et al. Plasma ghrelin and desacyl ghrelin concentrations in renal failure. J Am Soc Nephrol 2002; 13: 2748-52.CrossrefPubMedGoogle Scholar

  • Kumru S, Aydin S, Gursu MF, Ozcan Z. Changes of serum paraoxonase (an HDL-cholesterol-associated lipophilic antioxidant) and arylesterase activities in severe preeclamptic women. Eur J Obstet Gynecol Reprod Biol 2004; 114: 177-81.Google Scholar

  • Ng CJ, Wadleigh DJ, Gangopadhyay A, Hama S, Grijalva VR, Navab M, et al. Paraoxonase-2 is a ubiquitously expressed protein with antioxidant properties and is capable of preventing cell-mediated oxidative modification of low density lipoprotein. J Biol Chem 2001; 276: 44444-9.Google Scholar

  • Mackness B, Durrington PN, Mackness MI. Human serum paraoxonase. Gen Pharmacol 1998; 31: 329-36.CrossrefPubMedGoogle Scholar

  • WHO. The evidence is in: deworming helps meet the Millennium Development Goals. 2005; WHO/CDS/CPE/PVC/2005.12

  • Cummings DE, Weigle DS, Frayo RS, Breen PA, Ma MK, Dellinger EP, et al. Plasma ghrelin levels after diet-induced weight loss or gastric bypass surgery. N Engl J Med 2002; 346: 1623-30.Google Scholar

  • Eckerson HW, Wyte MC, La Du BN. The human serum paraoxonase/arylesterase polymorphism. Am J Hum Genet 1983; 35: 1126-38.Google Scholar

  • Haagen L, Brock A. A new automated method for phenotyping arylesterase (E.C.3.1.1.2.) based upon inhibition of enzymatic hydrolysis of 4-nitrophenyl acetate by phenyl acetate. Eur J Clin Chem Clim Biochem 1992; 30: 391-5.Google Scholar

  • Horoz M, Aslan M, Selek S, Koylu AO, Bolukbas C, Bolukbas FF, et al. PON1 status in haemodialysis patients and the impact of hepatitis C infection. Clin Biochem 2007; 40: 609-14.Web of SciencePubMedCrossrefGoogle Scholar

  • Toshinai K, Mondal MS, Nakazato M, Date Y, Murakami N, Kojima M, et al. Upregulation of Ghrelin expression in the stomach upon fasting, insulin-induced hypoglycemia, and leptin administration. Biochem Biophys Res Commun 2001; 281: 1220-5.Google Scholar

  • Ashraf A, Mick G, Meleth S, Wang X, McCormick K. Insulin treatment reduces pre-prandial plasma ghrelin concentrations in children with type 1 diabetes. Med Sci Monit 2007; 13: CR533-7.Google Scholar

  • De Vriese C, Hacquebard M, Gregoire F, Carpentier Y, Delporte C. Ghrelin interacts with human plasma lipoproteins. Endocrinology 2007; 148: 2355-62.Google Scholar

  • Kılıç E, Yazar S, Saraymen R. Lipid Peroxidation Level in Patients with Blastocystosis. Inönü Üniversitesi Tıp Fakültesi Dergisi 2003; 10: 1-3.Google Scholar

  • Adelekan DA, Thurnham DI, Adekile AD. Reduced antioxidant capacity in paediatric patients with homozygous sickle cell disease. Eur J Clin Nutr 1989; 43: 609-14.Google Scholar

  • Kılıç E, Yazar S, Saraymen R, Özbilge H. Serum Lipid Peroxidation Level in Patients with Taeniasis saginata. Türkiye Parazitoloji Dergisi 2004; 2: 91-3.Google Scholar

  • Primo-Parmo SL, Sorenson RC, Teiber J, La Du BN. The human serum paraoxonase/arylesterase gene (PON1) is one member of a multigene family. Genomics 1996; 33: 498-507.CrossrefGoogle Scholar

  • Mackness MI, Arrol S, Abbott C, Durrington PN. Protection of low-density lipoprotein against oxidative modification by high-density lipoprotein associated paraoxonase. Atherosclerosis 1993; 104: 129-35.Google Scholar

  • Nevin DN, Zambon A, Seidel SL, Motulsky AG. Role of genetic polymorphism of human plasma paraoxonase/arylesterase in hydrolysis of the insecticide metabolites chlorpyrifos oxon and paraoxon. Am J Hum Genet 1988; 43: 230-8.Google Scholar

  • Hong SH, Song J, Min WK, Kim JQ. Genetic variations of the paraoxonase gene in patients with coronary artery disease. Clin Biochem 2001; 34: 475-81.CrossrefPubMedGoogle Scholar

  • Marsillach J, Camps J, Ferré N, Beltran R, Rull A, Mackness B, et al. Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in experimental liver disease. BMC Gastroenterol 2009; 14: 9: 3.Web of ScienceGoogle Scholar

  • Aydin S, Guzel SP, Kumru S, Aydin S, Akin O, Kavak E, et al. Serum leptin and ghrelin concentrations of maternal serum, arterial and venous cord blood in healthy and preeclamptic pregnant women. J Physiol Biochem 2008; 64: 51-9.Web of ScienceCrossrefPubMedGoogle Scholar

  • Tsuzura S, Ikeda Y, Suehiro T, Ota K, Osaki F, Arii K, et al. Correlation of plasma oxidized low-density lipoprotein levels to vascular complications and human serum paraoxonase in patients with type 2 diabetes. Metabolism 2004; 53: 297-302.PubMedGoogle Scholar

About the article


Published Online: 2010-01-06

Published in Print: 2010-01-01


Citation Information: Journal of Medical Biochemistry, ISSN (Online) 1452-8266, ISSN (Print) 1452-8258, DOI: https://doi.org/10.2478/v10011-010-0004-0.

Export Citation

This content is open access.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Suleyman Aydin, Sebnem Erenler, and Yalcin Kendir
Journal of Medical Biochemistry, 2011, Volume 30, Number 4
[2]
Suleyman Aydin, Suna Aydin, Gerry Croteau, Íbrahim Sahin, and Cihan Citil
Journal of Medical Biochemistry, 2010, Volume 29, Number 2

Comments (0)

Please log in or register to comment.
Log in