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Journal of Pediatric Endocrinology and Metabolism

Editor-in-Chief: Kiess, Wieland

Ed. by Bereket, Abdullah / Darendeliler, Feyza / Dattani, Mehul / Gustafsson, Jan / Luo, Fei Hong / Mericq, Veronica / Toppari, Jorma

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Volume 26, Issue 5-6


The role of uncoupling protein 2 and 3 genes polymorphism and energy expenditure in obese Indonesian children

Maria Mexitalia
  • Corresponding author
  • Department of Pediatrics, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Semarang, Indonesia
  • Email
  • Other articles by this author:
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/ Taro Yamauchi
  • Laboratory of Human Ecology, Graduate School of Health Sciences, Hokkaido University, Sapporo, Japan
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  • De Gruyter OnlineGoogle Scholar
/ Agustini Utari
  • Department of Pediatrics, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Semarang, Indonesia
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/ Damayanti R. Sjarif
  • Department of Pediatrics, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia
  • Other articles by this author:
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/ Hertanto W. Subagio / Agustinus Soemantri
  • Department of Pediatrics, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Semarang, Indonesia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Takafumi Ishida
  • Laboratory of Human Biology and Genetic, Department of Biological Sciences, Graduate School of Sciences, University of Tokyo, Tokyo, Japan
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Published Online: 2013-02-15 | DOI: https://doi.org/10.1515/jpem-2012-0311


Aim: Uncoupling protein (UCP) genes, which may contribute to energy metabolism in mitochondria, may be involved in the pathogenesis of obesity. We analyzed the differences in energy expenditure between single nucleotide polymorphisms (SNPs) UCP3-55C/T, UCP3 Y210Y, and UCP2 A55V among Indonesian children.

Methods: The study included 76 schoolchildren (36 obese and 40 healthy; mean age, 12.8 years) in Semarang, Indonesia. Body composition was measured by bioelectrical impedance analysis; resting energy expenditure (REE) by indirect calorimetry; physical activity by uniaxial accelerometer; and total energy expenditure (TEE) by the equations extrapolated from REE and physical activity. UCP3-55C/T, UCP3 Y210Y, and UCP2 A55V were examined by restriction length fragment polymorphism analysis.

Results: The TEE of the subjects with the T/T genotype at UCP3-55C/T after adjusting for fat-free mass (63.2±7.2 kcal/kg/day) and T/T at UCP2 A55V (62.8±5.6 kcal/kg/day) was lower than that of the subjects with the C/C and C/T genotypes (p<0.05). The REE of the subjects with these T/T genotypes tended to be lower than that of the subjects with C/C and C/T (p≥0.05). No significant differences in REE or TEE were found between the UCP3 Y210Y genotypes.

Conclusions: The subjects with the T/T genotypes of UCP3-55C/T or UCP2 A55V had lower TEE than those with other genotypes.

Keywords: children; energy expenditure; Indonesia; obesity; UCP genes


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About the article

Corresponding author: Maria Mexitalia, Department of Pediatrics, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Dr. Sutomo 16–18 Semarang Central Java 50231, Semarang, Indonesia, Phone/Fax: +62248414296.

Received: 2012-10-04

Accepted: 2012-12-22

Published Online: 2013-02-15

Published in Print: 2013-05-01

Citation Information: Journal of Pediatric Endocrinology and Metabolism, Volume 26, Issue 5-6, Pages 441–447, ISSN (Online) 2191-0251, ISSN (Print) 0334-018X, DOI: https://doi.org/10.1515/jpem-2012-0311.

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