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Journal of Perinatal Medicine

Official Journal of the World Association of Perinatal Medicine

Editor-in-Chief: Dudenhausen, MD, FRCOG, Joachim W.

Ed. by Bancalari, Eduardo / Chappelle, Joseph / Chervenak, Frank A. / Genc, Mehmet R. / Greenough, Anne / Grunebaum, Amos / Konje, Justin C. / Kurjak M.D., Asim / Romero, Roberto

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CiteScore 2017: 1.26

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Volume 32, Issue 5


The effect of processing and cryopreservation on nucleated umbilical cord blood cells

Lucy A. Bayer-Zwirello
  • Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, St. Elizabeth’s Medical Center, Boston, Tufts University School of Medicine, Boston, Massachusetts, U.S.A.
  • Other articles by this author:
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/ Despina E. Hoffman / Lorrie A. Adams / Paul T. Wilder / Margaret T. Reece
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/JPM.2004.142


Recovery of nucleated cord blood cells after storage in liquid nitrogen was evaluated. Red cells were depleted using Ficoll-Paque™ or Puregene® red cell lyses. Freeze Medium contained 10% dimethylsulfoxide and 20% serum for cryoprotection. Recovery of the original cell population remaining serviceable for fluorescence activated cell sorting (FACS) was 12 ± 10% (average ± standard deviation), with a range of 1% to 55%. Viability measured by FACS analysis after freezing was significantly lower than that of the same specimens prior to freezing, 62 ± 20% compared to 91 ± 11% (p < 0.001). Percentage CD45+34+ cells were the same for fresh and frozen cells. Gestational age at which specimens were collected had no effect on the percent cells carrying the CD45+34+ markers. We conclude that better cryoprotective supplements are needed to insure consistent high recovery of viable nucleated umbilical cord blood cells after preservation in liquid nitrogen.

Keywords: Blood; cells; cryopreservation; nucleated; recovery; umbilical cord blood

About the article

Corresponding author: Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, St. Elizabeth’s Medical Center, 736 Cambridge Street, Boston, MA 02135/U.S.A. E-mail:

Received: July 15, 2003

Revised: May 14, 2004

Accepted: May 17, 2004

Published Online: 2005-06-01

Published in Print: 2004-09-01

Citation Information: Journal of Perinatal Medicine, Volume 32, Issue 5, Pages 430–433, ISSN (Print) 0300-5577, DOI: https://doi.org/10.1515/JPM.2004.142.

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