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Journal of Perinatal Medicine

Official Journal of the World Association of Perinatal Medicine

Editor-in-Chief: Dudenhausen, MD, FRCOG, Joachim W.

Editorial Board Member: / Bancalari, Eduardo / Greenough, Anne / Genc, Mehmet R. / Chervenak, Frank A. / Chappelle, Joseph / Bergmann, Renate L. / Bernardes, J.F. / Bevilacqua, G. / Blickstein, Isaac / Cabero Roura, Luis / Carbonell-Estrany, Xavier / Carrera, Jose M. / D`Addario, Vincenzo / D'Alton, MD, Mary E. / Dimitrou, G. / Grunebaum, Amos / Hentschel, Roland / Köpcke, W. / Kawabata, Ichiro / Keirse, Marc J.N.C. / Kurjak M.D., Asim / Lee, Ben H. / Levene, Malcolm / Lockwood, Charles J. / Marsal, Karel / Makatsariya, Alexander / Nishida, Hiroshi / Papp, Zoltán / Pejaver, Ranjan Kumar / Pooh, Ritsuko K. / Reiss, Irwin / Romero, Roberto / Saugstad, Ola D. / Schenker, Joseph G. / Sen, Cihat / Seri, Istvan / Vetter, Klaus / Winn, Hung N. / Young, Bruce K. / Zimmermann, Roland

9 Issues per year


IMPACT FACTOR 2016: 1.577
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CiteScore 2016: 1.49

SCImago Journal Rank (SJR) 2016: 0.602
Source Normalized Impact per Paper (SNIP) 2016: 0.832

Online
ISSN
1619-3997
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Volume 32, Issue 5 (Sep 2004)

Issues

The effect of processing and cryopreservation on nucleated umbilical cord blood cells

Lucy A. Bayer-Zwirello
  • Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, St. Elizabeth’s Medical Center, Boston, Tufts University School of Medicine, Boston, Massachusetts, U.S.A.
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/ Despina E. Hoffman / Lorrie A. Adams / Paul T. Wilder / Margaret T. Reece
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/JPM.2004.142

Abstract

Recovery of nucleated cord blood cells after storage in liquid nitrogen was evaluated. Red cells were depleted using Ficoll-Paque™ or Puregene® red cell lyses. Freeze Medium contained 10% dimethylsulfoxide and 20% serum for cryoprotection. Recovery of the original cell population remaining serviceable for fluorescence activated cell sorting (FACS) was 12 ± 10% (average ± standard deviation), with a range of 1% to 55%. Viability measured by FACS analysis after freezing was significantly lower than that of the same specimens prior to freezing, 62 ± 20% compared to 91 ± 11% (p < 0.001). Percentage CD45+34+ cells were the same for fresh and frozen cells. Gestational age at which specimens were collected had no effect on the percent cells carrying the CD45+34+ markers. We conclude that better cryoprotective supplements are needed to insure consistent high recovery of viable nucleated umbilical cord blood cells after preservation in liquid nitrogen.

Keywords: Blood; cells; cryopreservation; nucleated; recovery; umbilical cord blood

About the article

Corresponding author: Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, St. Elizabeth’s Medical Center, 736 Cambridge Street, Boston, MA 02135/U.S.A. E-mail:


Received: July 15, 2003

Revised: May 14, 2004

Accepted: May 17, 2004

Published Online: 2005-06-01

Published in Print: 2004-09-01


Citation Information: Journal of Perinatal Medicine, ISSN (Print) 0300-5577, DOI: https://doi.org/10.1515/JPM.2004.142.

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