Editor-in-Chief: Dudenhausen, MD, FRCOG, Joachim W.
Editorial Board Member: / Bancalari, Eduardo / Greenough, Anne / Genc, Mehmet R. / Chervenak, Frank A. / Chappelle, Joseph / Bergmann, Renate L. / Bernardes, J.F. / Bevilacqua, G. / Blickstein, Isaac / Cabero Roura, Luis / Carbonell-Estrany, Xavier / Carrera, Jose M. / D`Addario, Vincenzo / D'Alton, MD, Mary E. / Dimitrou, G. / Grunebaum, Amos / Hentschel, Roland / Köpcke, W. / Kawabata, Ichiro / Keirse, Marc J.N.C. / Kurjak M.D., Asim / Lee, Ben H. / Levene, Malcolm / Lockwood, Charles J. / Marsal, Karel / Makatsariya, Alexander / Nishida, Hiroshi / Papp, Zoltán / Pejaver, Ranjan Kumar / Pooh, Ritsuko K. / Reiss, Irwin / Romero, Roberto / Saugstad, Ola D. / Schenker, Joseph G. / Sen, Cihat / Seri, Istvan / Vetter, Klaus / Winn, Hung N. / Young, Bruce K. / Zimmermann, Roland
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Objective: Abnormal inflammatory responses are implicated in the pathogenesis of neonatal disease. This study aimed to describe the neonatal cytokine response using an in vitro model of stimulated cord blood.
Methods: Cord blood samples (n = 12) were incubated in RPMI 1640 medium with and without lipopolysaccharide. Concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8, interferon (IFN)-γ and IL-10 were determined by multiplex immunoassay at 0, 1, 3, 6 and 24 hours of incubation. The difference between stimulated and control response was defined as the potential secretory capacity (mean ± S.E.M.; pg/million white cells). Analysis included a Kruskal-Wallis test and post-hoc Mann-Whitney U test.
Results: All cytokine capacities increased rapidly by 1 hour (p < 0.001), except IL-10 (p = 0.04). TNF-α peaked between 3–6 hours (1581 ± 377 pg/million WC), declining by 24 hours. Similarly, IFN-γ peaked at 3 hours. Capacity ascended throughout the incubation period for IL-6, IL-8 (631 ± 75 pg/million WC) and IL-10 (311 ± 37 pg/million WC). Overall, IFN-γ capacity was lowest (72 ± 10 pg/million WC) and IL-6 capacity was greatest (61489 ± 7059 pg/million WC).
Conclusion: The neonatal inflammatory response is chronologically similar to that determined in adults. Immature neonatal T-cell function may account for the lower IFN-γ production. These results may expand our knowledge of neonatal disease, etiology and management.
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