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Journal of Perinatal Medicine

Official Journal of the World Association of Perinatal Medicine

Editor-in-Chief: Dudenhausen, MD, FRCOG, Joachim W.

Ed. by Bancalari, Eduardo / Chappelle, Joseph / Chervenak, Frank A. / D'Addario , Vincenzo / Genc, Mehmet R. / Greenough, Anne / Grunebaum, Amos / Konje, Justin C. / Kurjak M.D., Asim / Romero, Roberto / Zalud, MD PhD, Ivica

IMPACT FACTOR 2018: 1.361
5-year IMPACT FACTOR: 1.578

CiteScore 2018: 1.29

SCImago Journal Rank (SJR) 2018: 0.522
Source Normalized Impact per Paper (SNIP) 2018: 0.602

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Volume 33, Issue 5


Effects of arecoline in relaxing human umbilical vessels and inhibiting endothelial cell growth

Fu-Chen Kuo / Deng-Chyang Wu
  • Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Shyng-Shiou F. Yuan / Kwo-Ming Hsiao / Yuan-Yung Wang / Yuan-Chieh Yang / Yi-Ching Lo
  • Graduate Institute and Department of Pharmacology, Kaohsiung Medical University, Kaohsiung, Taiwan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2005-09-13 | DOI: https://doi.org/10.1515/JPM.2005.072


This study investigated the effects of arecoline, an active ingredient of the areca nut, on the tone of human umbilical arteries and veins and on the eNOS expression and cell proliferation of human umbilical vein endothelial cells (HUVECs). We found that arecoline relaxes the human umbilical artery and vein rings in a concentration-dependent manner; the higher the concentration of arecoline, the greater the relaxation of the rings. However, the relaxation decreases after the endothelium was removed or pretreated with L-NAME, a nitric oxide synthase inhibitor. Moreover, arecoline increases in a dose-dependent way the cGMP levels of human umbilical arteries and veins. In HUVECs, arecoline also increases the eNOS expression. Therefore, the relaxant effects of arecoline on the umbilical artery and vein rings were endothelium-dependent through the NO-cGMP systems. In addition, arecoline at higher doses (100–1000 μM) inhibits endothelial cell proliferation; the exposure toarecoline (100–1000 μM) for 24 and 48 h induces G2/M cell cycle arrest of HUVECs. Our results indicate that arecoline would decrease vascular tone, in part mediated by NO. Higher doses of arecoline inhibit endothelial cell growth, which suggest that long-term use or high doses of areca nut might induce endothelial dysfunction and associated diseases.

Keywords: Areca nut; arecoline; human umbilical arteries and veins; human umbilical vein endothelium cells

About the article

Corresponding author: Graduate Institute and Department of Pharmacology Kaohsiung Medical University No.100, Shih-Chuan 1st road Kaohsiung 807 Taiwan Tel.: 886-7-3121101 ext 2139 Fax: 886-7-3234686

Received: February 16, 2005

Revised: April 15, 2005

Accepted: April 23, 2005

Published Online: 2005-09-13

Published in Print: 2005-10-01

Citation Information: Journal of Perinatal Medicine, Volume 33, Issue 5, Pages 399–405, ISSN (Online) 1619-3997, ISSN (Print) 0300-5577, DOI: https://doi.org/10.1515/JPM.2005.072.

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