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Journal of Perinatal Medicine

Official Journal of the World Association of Perinatal Medicine

Editor-in-Chief: Dudenhausen, MD, FRCOG, Joachim W.

Ed. by Bancalari, Eduardo / Chappelle, Joseph / Chervenak, Frank A. / D'Addario , Vincenzo / Genc, Mehmet R. / Greenough, Anne / Grunebaum, Amos / Kurjak M.D., Asim / Romero, Roberto / Zalud, MD PhD, Ivica


IMPACT FACTOR 2018: 1.361
5-year IMPACT FACTOR: 1.578

CiteScore 2018: 1.29

SCImago Journal Rank (SJR) 2018: 0.522
Source Normalized Impact per Paper (SNIP) 2018: 0.602

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1619-3997
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Volume 37, Issue 4

Issues

Does progesterone inhibit bacteria-stimulated interleukin-8 production by lower genital tract epithelial cells?

Morgan R. Peltier
  • Perinatal Research Laboratory, Applied Bench Core, Winthrop University Hospital, Mineola, NY, USA
  • Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Winthrop University Hospital, Mineola, NY, USA
  • Division of Neonatology, Department of Pediatrics, Winthrop University Hospital, Mineola, NY, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Yana Berlin
  • Perinatal Research Laboratory, Applied Bench Core, Winthrop University Hospital, Mineola, NY, USA
  • Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, NJ, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Siew C. Tee
  • Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, NJ, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ John C. Smulian
  • Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Lehigh Valley Hospital, Allentown, PA, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2009-03-17 | DOI: https://doi.org/10.1515/JPM.2009.064

Abstract

Objective: Progesterone (P4) has been clinically shown to prevent the recurrence of preterm birth. The mechanism(s) of action is unclear, but may involve modulation of the immunologic inflammatory response of the lower genital tract. We evaluated the effects of P4 on interleukin-8 (IL-8) production by vaginal and cervical epithelial cells stimulated with bacterial species that are commonly associated with preterm birth.

Methods: Vaginal and endocervical epithelial cells were incubated with up to 10,000 ng/mL P4 overnight and stimulated with heat-killed Escherichia coli, Gardnerella vaginalis, or Ureaplasma urealyticum. Concentrations of IL-8 in conditioned medium were quantified by ELISA and viability of the cell cultures was measured by the reduction of a tetrazolium salt.

Results: E. coli, G. vaginalis and U. urealyticum-stimulated IL-8 production for both cell lines. P4 inhibited basal and bacteria-stimulated IL-8 production for vaginal epithelial cells but enhanced IL-8 production by endocervical cells. P4 reduced the number of viable cells for both cell lines.

Conclusions: P4 inhibits IL-8 production by vaginal epithelial cells stimulated with pathogens associated with preterm birth, possibly by reducing the number of viable cells or by inhibiting their proliferation. Although P4 also reduces proliferation of endocervical cells it also increases their production of IL-8.

Keywords: Infection; innate immunity; preterm birth; progesterone; vaginal immunity

About the article

Corresponding author: Morgan R. Peltier, PhD Perinatal Research Laboratory Applied Bench Core Winthrop University Hospital Mineola, NY 11501 USA Tel.: +1 (516) 663 2035 Fax: +1 (516) 663-8871


Received: 2008-09-30

Revised: 2008-11-24

Accepted: 2008-11-27

Published Online: 2009-03-17

Published Online: 2009-03-17

Published in Print: 2009-07-01


Citation Information: Journal of Perinatal Medicine, Volume 37, Issue 4, Pages 328–333, ISSN (Online) 1619-3997, ISSN (Print) 0300-5577, DOI: https://doi.org/10.1515/JPM.2009.064.

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