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Journal of Perinatal Medicine

Official Journal of the World Association of Perinatal Medicine

Editor-in-Chief: Dudenhausen, MD, FRCOG, Joachim W.

Editorial Board Member: / Bancalari, Eduardo / Greenough, Anne / Genc, Mehmet R. / Chervenak, Frank A. / Chappelle, Joseph / Bergmann, Renate L. / Bernardes, J.F. / Bevilacqua, G. / Blickstein, Isaac / Cabero Roura, Luis / Carbonell-Estrany, Xavier / Carrera, Jose M. / D`Addario, Vincenzo / D'Alton, MD, Mary E. / Dimitrou, G. / Grunebaum, Amos / Hentschel, Roland / Köpcke, W. / Kawabata, Ichiro / Keirse, Marc J.N.C. / Kurjak M.D., Asim / Lee, Ben H. / Levene, Malcolm / Lockwood, Charles J. / Marsal, Karel / Makatsariya, Alexander / Nishida, Hiroshi / Papp, Zoltán / Pejaver, Ranjan Kumar / Pooh, Ritsuko K. / Reiss, Irwin / Romero, Roberto / Saugstad, Ola D. / Schenker, Joseph G. / Sen, Cihat / Seri, Istvan / Vetter, Klaus / Winn, Hung N. / Young, Bruce K. / Zimmermann, Roland

9 Issues per year


IMPACT FACTOR 2016: 1.577
5-year IMPACT FACTOR: 1.705

CiteScore 2016: 1.49

SCImago Journal Rank (SJR) 2016: 0.602
Source Normalized Impact per Paper (SNIP) 2016: 0.832

Online
ISSN
1619-3997
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Volume 38, Issue 5 (Sep 2010)

Issues

Microbial invasion of the amniotic cavity in pregnancies with small-for-gestational-age fetuses

Daniel B. DiGiulio
  • Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA
  • Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Maria Teresa Gervasi / Roberto Romero
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Hutzel Women's Hospital, Detroit Medical Center, Detroit, MI, USA
  • Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Edi Vaisbuch
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Shali Mazaki-Tovi
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Juan Pedro Kusanovic
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kimberley S. Seok / Ricardo Gómez
  • CEDIP (Center for Perinatal Diagnosis and Research), Department of Obstetrics and Gynecology, Sotero del Rio Hospital, Santiago, Chile
  • Department of Obstetrics and Gynecology, P. Universidad Católica de Chile, Santiago, Chile
  • Other articles by this author:
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/ Pooja Mittal
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA
  • Other articles by this author:
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/ Francesca Gotsch / Tinnakorn Chaiworapongsa
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Enrique Oyarzún / Chong Jai Kim
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Department of Pathology, Wayne State University School of Medicine, Detroit, MI, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ David A. Relman
  • Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA
  • Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA
  • Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2010-05-20 | DOI: https://doi.org/10.1515/jpm.2010.076

Abstract

Objective: Microbial invasion of the amniotic cavity (MIAC) has been detected in women with preterm labor, preterm prelabor rupture of membranes (PROM), and in patients at term with PROM or in spontaneous labor. Intrauterine infection is recognized as a potential cause of fetal growth restriction; yet, the frequency of MIAC in pregnancies with small-for-gestational-age (SGA) fetuses is unknown. The aim of this study was to determine the frequency, diversity and relative abundance of microbes in amniotic fluid (AF) of women with an SGA neonate using a combination of culture and molecular methods.

Method: AF from 52 subjects with an SGA neonate was analyzed with both cultivation and molecular methods in a retrospective cohort study. Broad-range and group-specific PCR assays targeted small subunit rDNA, or other gene sequences, from bacteria, fungi and archaea. Results of microbiologic studies were correlated with indices of the host inflammatory response.

Results: 1) All AF samples (n=52) were negative for microorganisms based on cultivation techniques, whereas 6% (3/52) were positive based on PCR; and 2) intra-amniotic inflammation was detected in one of the three patients with a positive PCR result, as compared with three patients (6.1%) of the 49 with both a negative culture and a negative PCR (P=0.2).

Conclusion: MIAC is detected by PCR in some patients with an SGA fetus who were not in labor at the time of AF collection.

Keywords: 16S rRNA; chorioamnionitis; cytokines; FIRS; IL-6; intra-amniotic infection; intra-amniotic inflammation; molecular microbiology; PCR; pregnancy; SGA

About the article

Corresponding author: Roberto Romero, MD Perinatology Research Branch Intramural Division NICHD/NIH/DHHS Hutzel Women's Hospital Box No. 4, 3990 John R, Detroit MI 48201 USA Tel.: +1 (313) 993-2700 Fax: +1 (313) 993-2694


Received: 2010-03-05

Revised: 2010-03-26

Accepted: 2010-03-29

Published Online: 2010-05-20

Published Online: 2010-05-20

Published in Print: 2010-09-01


Citation Information: Journal of Perinatal Medicine, ISSN (Online) 1619-3997, ISSN (Print) 0300-5577, DOI: https://doi.org/10.1515/jpm.2010.076.

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Nardhy Gomez-Lopez, Roberto Romero, Yi Xu, Yaozhu Leng, Valeria Garcia-Flores, Derek Miller, Suzanne M. Jacques, Sonia S. Hassan, Jonathan Faro, Adham Alsamsam, Ali Alhousseini, Hunter Gomez-Roberts, Bogdan Panaitescu, Lami Yeo, and Eli Maymon
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Nardhy Gomez-Lopez, Roberto Romero, Valeria Garcia-Flores, Yi Xu, Yaozhu Leng, Ali Alhousseini, Sonia S. Hassan, and Bogdan Panaitescu
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Chong Jai Kim, Roberto Romero, Piya Chaemsaithong, Noppadol Chaiyasit, Bo Hyun Yoon, and Yeon Mee Kim
American Journal of Obstetrics and Gynecology, 2015, Volume 213, Number 4, Page S29
[7]
Roberto Romero, Jean-Charles Grivel, Adi L. Tarca, Piya Chaemsaithong, Zhonghui Xu, Wendy Fitzgerald, Sonia S. Hassan, Tinnakorn Chaiworapongsa, and Leonid Margolis
American Journal of Obstetrics and Gynecology, 2015, Volume 213, Number 6, Page 836.e1
[8]
Matthew S. Payne and Sara Bayatibojakhi
Frontiers in Immunology, 2014, Volume 5
[9]
Roberto Romero, Jezid Miranda, Tinnakorn Chaiworapongsa, Piya Chaemsaithong, Francesca Gotsch, Zhong Dong, Ahmed I. Ahmed, Bo Hyun Yoon, Sonia S. Hassan, Chong Jai Kim, Steven J. Korzeniewski, and Lami Yeo
American Journal of Reproductive Immunology, 2014, Volume 71, Number 4, Page 330
[10]
Roberto Murgas Torrazza and Josef Neu
Clinics in Perinatology, 2013, Volume 40, Number 1, Page 93
[11]
Daniel B. DiGiulio
Seminars in Fetal and Neonatal Medicine, 2012, Volume 17, Number 1, Page 2
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J. M. Goldstein, S. Cherkerzian, S. L. Buka, G. Fitzmaurice, M. Hornig, M. Gillman, S. O'Toole, and R. P. Sloan
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RF Lamont, JD Sobel, RA Akins, SS Hassan, T Chaiworapongsa, JP Kusanovic, and R Romero
BJOG: An International Journal of Obstetrics & Gynaecology, 2011, Volume 118, Number 5, Page 533
[14]
Ingrid Cardenas, Gil Mor, Paulomi Aldo, Sabine M. Lang, Paul Stabach, Andrew Sharp, Roberto Romero, Shali Mazaki-Tovi, MariaTeresa Gervasi, and Robert E. Means
American Journal of Reproductive Immunology, 2011, Volume 65, Number 2, Page 110

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