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Journal of Perinatal Medicine

Official Journal of the World Association of Perinatal Medicine

Editor-in-Chief: Dudenhausen, MD, FRCOG, Joachim W.

Editorial Board Member: / Bancalari, Eduardo / Greenough, Anne / Genc, Mehmet R. / Chervenak, Frank A. / Chappelle, Joseph / Bergmann, Renate L. / Bernardes, J.F. / Bevilacqua, G. / Blickstein, Isaac / Cabero Roura, Luis / Carbonell-Estrany, Xavier / Carrera, Jose M. / D`Addario, Vincenzo / D'Alton, MD, Mary E. / Dimitrou, G. / Grunebaum, Amos / Hentschel, Roland / Köpcke, W. / Kawabata, Ichiro / Keirse, Marc J.N.C. / Kurjak M.D., Asim / Lee, Ben H. / Levene, Malcolm / Lockwood, Charles J. / Marsal, Karel / Makatsariya, Alexander / Nishida, Hiroshi / Papp, Zoltán / Pejaver, Ranjan Kumar / Pooh, Ritsuko K. / Romero, Roberto / Saugstad, Ola D. / Schenker, Joseph G. / Sen, Cihat / Seri, Istvan / Vetter, Klaus / Winn, Hung N. / Young, Bruce K. / Zimmermann, Roland

9 Issues per year


IMPACT FACTOR 2016: 1.577
5-year IMPACT FACTOR: 1.705

CiteScore 2016: 1.49

SCImago Journal Rank (SJR) 2016: 0.602
Source Normalized Impact per Paper (SNIP) 2016: 0.832

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1619-3997
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Volume 38, Issue 5 (Sep 2010)

Issues

Microbial invasion of the amniotic cavity in preeclampsia as assessed by cultivation and sequence-based methods

Daniel B. DiGiulio
  • Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA
  • Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA
  • Other articles by this author:
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/ MariaTeresa Gervasi / Roberto Romero
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Hutzel Women's Hospital, Detroit Medical Center, Detroit, MI, USA
  • Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA
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/ Shali Mazaki-Tovi
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Hutzel Women's Hospital, Detroit Medical Center, Detroit, MI, USA
  • Other articles by this author:
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/ Edi Vaisbuch
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Hutzel Women's Hospital, Detroit Medical Center, Detroit, MI, USA
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/ Juan Pedro Kusanovic
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Hutzel Women's Hospital, Detroit Medical Center, Detroit, MI, USA
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/ Kimberley S. Seok / Ricardo Gómez
  • CEDIP (Center for Perinatal Diagnosis and Research), Department of Obstetrics and Gynecology, Sotero del Rio Hospital, Santiago, Chile
  • Department of Obstetrics and Gynecology, P. Universidad Católica de Chile, Santiago, Chile
  • Other articles by this author:
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/ Pooja Mittal
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Hutzel Women's Hospital, Detroit Medical Center, Detroit, MI, USA
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/ Francesca Gotsch / Tinnakorn Chaiworapongsa
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Hutzel Women's Hospital, Detroit Medical Center, Detroit, MI, USA
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/ Enrique Oyarzún / Chong Jai Kim
  • Perinatology Research Branch, NICHD, NIH, Bethesda, MD, and Detroit, MI, USA
  • Department of Pathology, Wayne State University School of Medicine, Detroit, MI, USA
  • Other articles by this author:
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/ David A. Relman
  • Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA
  • Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA
  • Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA
  • Other articles by this author:
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Published Online: 2010-05-20 | DOI: https://doi.org/10.1515/jpm.2010.078

Abstract

Objective: Infection has been implicated in the pathogenesis of preeclampsia, yet the association between microbial invasion of the amniotic cavity (MIAC) and preeclampsia has not been determined. The aim of this study was to determine the prevalence, and microbial diversity associated with MIAC, as well as the nature of the host response to MIAC in patients with preeclampsia.

Method of study: Amniotic fluid (AF) from 62 subjects with preeclampsia, not in labor, was analyzed with both cultivation and molecular methods. Broad-range and group-specific PCR assays targeting small subunit ribosomal DNA, or other gene sequences, from bacteria, fungi and archaea were used. Results were correlated with measurements of host inflammatory response, including AF white blood cell count and AF concentrations of glucose, interleukin-6 (IL-6) and MMP-8.

Results: 1) The rate of MIAC in preeclampsia was 1.6% (1/62) based on cultivation techniques, 8% (5/62) based on PCR, and 9.6% (6/62) based on the combined results of both methods; 2) among the six patients diagnosed with MIAC, three had a positive PCR for Sneathia/Leptotrichia spp.; and 3) patients with MIAC were more likely to have evidence of an inflammatory response in the amniotic cavity than those without MIAC, as determined by a higher median AF IL-6 [1.65 ng/mL interquartile range (IQR): 0.35–4.62 vs. 0.22 ng/mL IQR: 0.12–0.51; P=0.002).

Conclusion: The prevalence of MIAC in preeclampsia is low, suggesting that intra-amniotic infection plays only a limited role in preeclampsia. However, the unexpectedly high number of positive AF specimens for Sneathia/Leptotrichia warrants further investigation.

Keywords: IL-6; intra-amniotic infection; intra-amniotic inflammation; PCR; preeclampsia; pregnancy; 16S rRNA; Sneathia/Leptotrichia spp; Ureaplasma urealyticum

About the article

Corresponding author: Roberto Romero, MD Perinatology Research Branch Intramural Division NICHD/NIH/DHHS Hutzel Women's Hospital Box No. 4, 3990 John R Detroit MI 48201 USA Tel.: +1 (313) 993-2700 Fax: +1 (313) 993-2694


Received: 2010-03-05

Revised: 2010-03-26

Accepted: 2010-03-29

Published Online: 2010-05-20

Published Online: 2010-05-20

Published in Print: 2010-09-01


Citation Information: Journal of Perinatal Medicine, ISSN (Online) 1619-3997, ISSN (Print) 0300-5577, DOI: https://doi.org/10.1515/jpm.2010.078.

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