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Journal of Perinatal Medicine

Official Journal of the World Association of Perinatal Medicine

Editor-in-Chief: Dudenhausen, MD, FRCOG, Joachim W.

Ed. by Bancalari, Eduardo / Chappelle, Joseph / Chervenak, Frank A. / D'Addario , Vincenzo / Genc, Mehmet R. / Greenough, Anne / Grunebaum, Amos / Konje, Justin C. / Kurjak M.D., Asim / Romero, Roberto / Zalud, MD PhD, Ivica


IMPACT FACTOR 2018: 1.361
5-year IMPACT FACTOR: 1.578

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1619-3997
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Volume 47, Issue 5

Issues

Expression of genes involved in inflammation and growth – does sampling site in human full-term placenta matter?

Marianne Allbrand
  • Corresponding author
  • Department of Obstetrics and Gynecology, School of Medical Sciences, Örebro University, Örebro, Sweden
  • Email
  • Other articles by this author:
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/ Jan Åman / Kerstin Nilsson
  • Department of Obstetrics and Gynecology, School of Medical Sciences, Örebro University, Örebro, Sweden
  • Other articles by this author:
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/ Yang CaoORCID iD: https://orcid.org/0000-0002-3552-9153 / Maria Lodefalk
  • Department of Pediatrics, School of Medical Sciences, Örebro University, Örebro, Sweden
  • University Health Care Research Center, Faculty of Medicine and Health, Örebro University, Örebro, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2019-03-28 | DOI: https://doi.org/10.1515/jpm-2018-0290

Abstract

Objective

To investigate the placental gene expression of substances in the inflammatory cascade and growth factors at nine different well-defined sampling sites in full-term placentas from 12 normal weight healthy non-smoking women with an uncomplicated singleton pregnancy.

Methods

All placentas (six girls and six boys) were delivered vaginally. Quantitative real-time polymerase chain reaction was used to analyze toll receptor-2 and -4, interleukin-6 and -8, tumor necrosis factor-α, leptin, ghrelin, insulin-like growth factor-1 and -2, hepatocyte growth factor, hepatocyte growth factor receptor and insulin receptor (IR).

Results

The leptin gene and the IR gene showed higher expression in lateral regions near the chorionic plate compared to central regions near the basal plate (P = 0.028 and P = 0.041, respectively).

Conclusion

Our results suggest that the sampling site may influence the gene expression for leptin and IR in placental tissue obtained from full-term normal pregnancies. We speculate that this may be due to differences in placental structure and perfusion and may be important when future studies are designed.

Keywords: cytokines; gene expression; growth factors; placenta; sampling

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About the article

Corresponding author: Marianne Allbrand, MS, Department of Obstetrics and Gynecology, School of Medical Sciences, Örebro University, Örebro, Sweden


Received: 2018-09-03

Accepted: 2019-01-31

Published Online: 2019-03-28

Published in Print: 2019-07-26


Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: This work received grants from the Research Committee of Region Örebro County and from Nyckelfonden, Örebro University Hospital.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Journal of Perinatal Medicine, Volume 47, Issue 5, Pages 539–546, ISSN (Online) 1619-3997, ISSN (Print) 0300-5577, DOI: https://doi.org/10.1515/jpm-2018-0290.

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