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Journal of Plant Protection Research

The Journal of Polish Society of Plant Protection, Committee of Plant Protection; Polish Academy of Sciences, Institute of Plant Protection – National Research Institute

4 Issues per year


CiteScore 2016: 0.84

SCImago Journal Rank (SJR) 2016: 0.332
Source Normalized Impact per Paper (SNIP) 2016: 0.829

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1899-007X
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Volume 49, Issue 1

Issues

Assessment of Phytotoxic Spent Broth and Spores of Alternaria Alternata (LC#508) As a Formulation with Enhanced Mycoherbicidal Activity to Control Lantana Camara

Sanjai Saxena
  • Department of Biotechnology and Environmental Sciences, Thapar University, Patiala, Punjab 147004, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Akhilesh Pandey
  • Department of Biological Sciences, Rani Durgavati Vishwavidyalaya, Jabalpur, Madhya Prades, 482001, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2009-04-02 | DOI: https://doi.org/10.2478/v10045-009-0001-9

Assessment of Phytotoxic Spent Broth and Spores of Alternaria Alternata (LC#508) As a Formulation with Enhanced Mycoherbicidal Activity to Control Lantana Camara

Enhanced social concern towards the ill effects of synthetic agrochemicals, their residual toxicity and resistance development in the target pests created a necessity of exploration of alternatives. Fungal biocontrol agents have been used as herbicides over two decades and research continues to enhance their efficacy comparable to synthetic herbicides. Combinations of synthetic herbicides with the fungal biocontrol agents was an approach adopted to enhance mycoherbicidal activity but had limited acceptance due to negative effects of their synthetic components. Hence a necessity for development of the formulations having least toxicity or being benign to non-target organisms was felt. The present study brings forth a new dimension of holistic and ecofriendly bioherbicidal formulations comprising of crude phytotoxins and spores of Alternaria alternata LC# 508 which enhances outstandingly its mycoherbicidal potential. Spent broth of Alternaria alternata (LC#508) exhibited toxic activity to its spores (autotoxicity) at a concentration of 50μg/ml which was used for developing five formulations FN1 to FN5. Formulations No. 5 was the best of all the formulations when evaluated by pot trails compared to control and other formulations. Disease onset and mortality was observed in 3.5 days and 5 days respectively in case of formulation No 5 (50 μg of crude spent broth + 1x 106 spores/ml) when compared to spore spray alone. Thus the formulation of crude spent broth and fungal spores could be developed as a cost effective and efficacious bioherbicide formulation when compared to the spores alone.

Badanie Półpłynnego Podłoża I Zarodników Alternaria Alternana Jako Formulacji O Wzmożonej Aktywności Mykoherbicydowej (LC#508) Do Zwalczania Lantana Camara

Wzmożone zainteresowanie społeczne oraz niekorzystne efekty syntetycznych agrochemikaliów, toksyczność ich pozostałości i rozwój odporności u zwalczanych agrofagów stworzyły potrzebę poszukiwania alternatywnych rozwiązań. W ciągu 20 lat wykorzystano grzyby będące czynnikami biologicznego zwalczania, a badania te są kontynuowane w celu uzyskiwania aktywności porównywanej z aktywnością herbicydów syntetycznych. Kombinacja herbicydów syntetycznych z grzybowymi czynnikami biologicznego zwalczania była zaadoptowanym podejściem mającym na celu pobudzenie aktywności mykoherbicydowej, ale z powodu negatywnych efektów fungicydów syntetycznych uzyskała ona ograniczoną akceptację. Uznano więc, że istnieje potrzeba opracowania formulacji mających jak najmniejszą toksyczność w stosunku do organizmów nie będących przedmiotem zwalczania. Prezentowane badania ukazują nowy wymiar holistycznej i przyjaznej środowisku formulacji bioherbicydowych w porównaniu do surowych fitotoksyn i zarodników A. alternanta LC#508, znacząco pobudzających potencjał mykoherbicydowy. Wykorzystane półpłynne podłoże wzrostowe A. alternata LC#508 wykazywało bardziej toksyczną aktywność niż jego zarodniki (autotoksyczność) w stężeniu 50 μlg/ml, które użyto do opracowania pięciu formulacji FN1 do FN5. Formulacja nr 5 była najlepsza w ocenianych doświadczeniach wazonowych, w porównaniu do kontroli oraz innych formulacji. Wystąpienie choroby i śmiertelność obserwowano odpowiednio po 3,5 i 5 dniach, w przypadku formulacji nr 5 (50 μlg surowej wykorzystanej pożywki półpłynnej + 1 x 106 zarodników/ml), w porównaniu do opryskiwania tylko zarodnikami. Istnieje więc możliwość opracowania ekonomicznie opłacalnej formulacji będącej skutecznym mykoherbicydem, porównywalnej do zarodników grzyba.

Keywords: Alternaria alternata; autotoxicity; fungal biocontrol agents; formulations; Lantana; mycoherbicides; phytotoxins

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About the article


Published Online: 2009-04-02

Published in Print: 2009-03-01


Citation Information: Journal of Plant Protection Research, Volume 49, Issue 1, Pages 1–5, ISSN (Online) 1899-007X, ISSN (Print) 1427-4345, DOI: https://doi.org/10.2478/v10045-009-0001-9.

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