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Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

4 Issues per year

IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

CiteScore 2016: 0.46

SCImago Journal Rank (SJR) 2015: 0.230
Source Normalized Impact per Paper (SNIP) 2015: 0.383

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Application of FTA® Cards for detection and storage of avian influenza virus

Michał Jóźwiak / Krzysztof Wyrostek / Katarzyna Domańska-Blicharz / Monika Olszewska-Tomczyk / Krzysztof Śmietanka / Zenon Minta
Published Online: 2016-04-29 | DOI: https://doi.org/10.1515/jvetres-2016-0001


Introduction: The aim of the study was to test the utility of Flinders Technology Associates filter paper (FTA® Cards) for molecular detection and storage of avian influenza virus (AIV). Material and Methods: There were two strains of AIV used in the study: low pathogenicity H7N1 and high pathogenicity H5N1 subtypes. Detection of viral material was conducted using molecular RT-PCR and rRT- PCR method. Results: The infectivity of LPAIV/H7N1 and HPAIV/H5N1 was completely inactivated within 1 h and 24 h after adsorption to FTA® Cards at room temperature, respectively. Viruses stored on FTA® Cards had detection limit approximately 1 log10 lower than live viruses. Viral RNA of both strains were detectable on the cards by rRT-PCR for a minimum of 150 d, irrespectively of storage temperatures (room temperature, -20ºC). RNA was also detected in all samples obtained from SPF chickens experimentally infected with HPAI/H5N1 on 3rd and 4th day post-infection (p.i.).

Conclusion: FTA® Cards enable safe and effective alternative transport of samples for molecular diagnosis of AIV.

Keywords: avian influenza virus; FTA® Cards; molecular detection; storage


  • 1. Abdelwhab E.M., Luschow D., Harder T.C., Hafez H.M.: The use of FTA® filter papers for diagnosis of avian influenza virus. J Virol Methods 2011, 174, 120-122.Web of ScienceGoogle Scholar

  • 2. Act of 11 March 2004 on animal health protection and fighting against infectious animal diseases (Journal of Laws 2004, No. 69, item 625 with subsequent amendments).Google Scholar

  • 3. Cortes A.L., Montiel E.R., Gimeno I.M.: Validation of Marek’s disease diagnosis and monitoring of Marek’s disease vaccines from samples collected in FTA® Cards. Avian Dis 2009, 54, 510-516.CrossrefWeb of ScienceGoogle Scholar

  • 4. Kalthoff D., Globie A., Beer M.: (Highly pathogenic) avian influenza as a zoonotic agent. Vet Microbiol 2010, 140, (3-4), 237-245.Web of ScienceGoogle Scholar

  • 5. Keeler S., Ferro P., Brown J., Jackwood M., Stallknecht D.: Use of FTA® Sampling Cards for molecular detection of avian influenza virus in wild birds. Avian Dis 2012, 56, 200-207.Web of ScienceGoogle Scholar

  • 6. Kraus R.H., van Hooft P., Waldenström J., Latorre-Margalef N., Ydenberg R.C., Prins H.H.: Avian influenza surveillance with FTA Cards. Field methods, biosafety, and transportation issues solved. J Visual Exp 2011, 54, 1-5.Google Scholar

  • 7. Moscoso H., Alvarado I., Hofacre C.L.: Molecular analysis of infectious bursal disease virus from bursal tissues collected on FTA® filter paper. Avian Dis 2006, 50, 391-396.CrossrefGoogle Scholar

  • 8. Moscoso H., Raybon E.O., Thayer S.G., Hofacre C.L.: Molecular detection and serotyping of infectious bronchitis virus from FTA® filter paper. Avian Dis 2005 49, 24-29.CrossrefGoogle Scholar

  • 9. Narayanan M.S., Parthiban M., Sathiya P., Kumanan K.: Molecular detection of Newcastle disease virus using Flinder Technology Associated-PCR. Vet Arch 2010, 80, 51-60.Google Scholar

  • 10. Natarajan P., Trinh T., Mertz L., Goldsborough M., Fox D.K.: Paper-based archiving of mammalian and plant samples for RNA analysis. BioTechniques 2000, 29, 1328-1333.Google Scholar

  • 11. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (Mammals, Birds and Bees). World Organization for Animal Health, Paris, 2015, Chapter 2.3.4, 1- 23.Google Scholar

  • 12. Peiris M.J.S., De Jong D.M., Guan Yi.: Avian influenza virus (H5N1) a threat to human health. Clin Microbiol Rev 2007, 20, (2), 243-267.CrossrefWeb of ScienceGoogle Scholar

  • 13. Perozo F., Villegas P., Estevez C., Alvarado I., Purvis L.B.: Use of FTA filter paper for the molecular detection of Newcastle disease virus. Avian Pathol 2006, 35, 93-98.CrossrefGoogle Scholar

  • 14. Scientific Report of EFSA. Higly pathogenic avian influenza A subtype H5N8. EFSA J 2014, 12, 39-41.Google Scholar

  • 15. Spackman E., Senne D.A., Myers T.J., Bulaga L.L., Garber L.P., Perdue M.L.: Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J Clin Microbiol 2002, 40, 3, 256-3260.Google Scholar

  • 16. Starick E., Römer-Oberdörfer A. Werner O.: Type- and subtype-specific RT-PCR assays for avian influenza A viruses (AIV). J Vet Med 2000, 47, 4, 295-301.Google Scholar

About the article

Received: 2015-11-10

Accepted: 2016-03-10

Published Online: 2016-04-29

Published in Print: 2016-03-01

Citation Information: Journal of Veterinary Research, Volume 60, Issue 1, Pages 1–6, ISSN (Online) 2450-8608, DOI: https://doi.org/10.1515/jvetres-2016-0001.

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© 2016. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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