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Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

4 Issues per year

IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

CiteScore 2016: 0.46

SCImago Journal Rank (SJR) 2015: 0.230
Source Normalized Impact per Paper (SNIP) 2015: 0.383

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Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Aliasghar Bahari / Masoud Sabouri Ghannad
  • Department of Microbiology, Faculty of Medicine, Hamedan University of Medical Sciences, 65176-19654 Hamedan, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Omid Dezfoulian
  • Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, 68137-17133 Khoramabad, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Fereydon Rezazadeh
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz University, 51666-16471 Tabriz, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ali Sadeghi-Nasab
  • Department of Clinical Sciences, Faculty of Veterinary Science, Bu-Ali Sina University, 65176-58978 Hamedan, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-04-29 | DOI: https://doi.org/10.1515/jvetres-2016-0002


Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood samples of sheep, and compare the results to those of histopathological examinations. Material and Methods: Eighty blood samples from clinically healthy sheep were randomly collected before the animals were slaughtered. Ten tissue samples from each lung and associated caudal mediastinal lymph node were taken. Results: Fifteen (18.75%) blood samples were found to contain proviral DNA, and 11 (13.75%) corresponding lung samples showed microscopic changes consistent with ovine pulmonary adenocarcinoma. None of the samples displayed metastases to the caudal mediastinal lymph nodes. The prominent pattern of neoplastic nodules consisted of acinar (alveolar) form. Conclusion: The results indicated the higher sensitivity of real-time PCR compared to histopathological examinations in detection of ovine pulmonary adenocarcinoma.

Keywords: sheep; ovine pulmonary adenocarcinoma; Jaagsiekte retrovirus; real-time PCR; histopathology


  • 1. Begin R., Rola-Pleszczynski M., Sirois P., Masse S., Nadeau D., Bureau M.A.: Sequential analysis of the bronchoalveolar milieu in conscious sheep. J Appl Physiol 1981, 50, 665-671.Google Scholar

  • 2. Beytut E., Sözmen M., Erginsoy S.: Immunohistochemical detection of pulmonary surfactant proteins and retroviral antigens in the lungs of sheep with pulmonary adenomatosis. J Comp Pathol 2009, 140, 43-53.Web of ScienceGoogle Scholar

  • 3. Caporale M., Centorame P., Giovannini A., Sacchini F., Di Ventura M., De las Heras M., Palmarini M.: Infection of lung epithelial cells and induction of pulmonary adenocarcinoma is not the most common outcome of naturally occurring JSRV infection during the commercial lifespan of sheep. Virology 2005, 338, 144-153.Google Scholar

  • 4. Cousens C., Gibson L., Finlayson J., Pritchard I., Dagleish M.P.: Prevalence of ovine pulmonary adenocarcinoma (Jaagsiekte) in a UK slaughterhouse sheep study. Vet Rec 2015, 176, 413.Web of ScienceGoogle Scholar

  • 5. De las Heras M., Gonzalez L., Sharp J.M.: Pathology of ovine pulmonary adenocarcinoma. Curr Top Microbiol Immunol 2003, 275, 25-54.Google Scholar

  • 6. De Martini J.C., Rosadio R.H., Lairmore M.D.: The etiology and pathogenesis of ovine pulmonary carcinoma (sheep pulmonary adenomatosis). Vet Microbiol 1988, 17, 219-236.Google Scholar

  • 7. De Martini J.C., York D.F.: Retrovirus-associated neoplasms of the respiratory system of sheep and goats. Ovine pulmonary carcinoma and enzootic nasal tumor. Vet Clin North Am Food Anim Pract 1997, 13, 55-70.Google Scholar

  • 8. Garcia-Goti M., Gonzalez L., Cousens C., Cortabarria N., Extramiana A.B., Minguijon E., Ortin A., De las Heras M., Sharp J.M.: Sheep pulmonary adenomatosis: characterization of two pathological forms associated with jaagsiekte retrovirus. J Comp Pathol 2000, 122, 55-65.Google Scholar

  • 9. Gonzalez L., Garcia-Goti M., Cousens C., Dewar P., Cortabarrıa N., Extramiana A.B., Ortin A., De Las Heras M., Sharp J.M.: Jaagsiekte sheep retrovirus can be detected in the peripheral blood during the pre-clinical period of sheep pulmonary adenomatosis. J Gen Virol 2001, 82, 1355-1358.Google Scholar

  • 10. Griffiths D.J., Martineau H.M., Cousens C.: Pathology and pathogenesis of ovine pulmonary adenocarcinoma. J Comp Pathol 2010, 142, 260-283.Web of ScienceGoogle Scholar

  • 11. Hoffmann B., Beer M., Reid S.M., Mertens P., Oura C.A., Van Rijn P.A., Slomka M.J., Banks J., Brown I.H., Alexander D.J., King D.P.: A review of RT-PCR technologies used in veterinary virology and disease control: sensitive and specific diagnosis of five livestock diseases notifiable to the World Organisation for Animal Health. Vet Microbiol 2009, 139, 1-23.Web of ScienceGoogle Scholar

  • 12. Holland M.J., Palmarini M., Garcia-Goti M., Gonzalez L., McKendrick I., De Las Heras M., Sharp J.M.: Jaagsiekte retrovirus is widely distributed both in T and B lymphocytes and in mononuclear phagocytes of sheep with naturally and experimentally acquired pulmonary adenomatosis. J Virol 1999, 73, 4004-4008.Google Scholar

  • 13. Khodakaram-Tafti A., Mohammadi A., Mansourian M.: Detection of the Jaagsiekte Sheep Retrovirus by RT-PCR in naturally ovine pulmonary adenocarcinoma-affected sheep and in apparently healthy in contact-sheep. Rev Med Vet 2009, 160, 319-323.Google Scholar

  • 14. Khodakaram-Tafti A., Razavi Z.: Morphopathological study of naturally occurring ovine pulmonary adenocarcinoma in sheep in Fars province, Iran. Iranian J Vet Res 2010, 11, 134-138.Google Scholar

  • 15. Kycko A., Reichert M.: PCR-based methods for detection of JSRV in experimentally and naturally infected sheep. Bull Vet Inst Pulawy 2010, 54, 445-450.Google Scholar

  • 16. Lewis F.I., Brülisauer F., Cousens C., McKendrick I.J., Gunn G.J.: Diagnostic accuracy of PCR for jaagsiekte sheep retrovirus using field data from 125 Scottish sheep flocks. Vet J 2011, 187, 104-108.Web of ScienceGoogle Scholar

  • 17. Mackay J.M., Arden K.E., Nitsche A.: Real-time PCR in virology. Nucleic Acids Res 2002, 30, 1292-1305.Google Scholar

  • 18. Mackay J.M., Nisbet D.I.: Jaagsiekte - a hazard of intensified sheep husbandry. Vet Rec 1966, 78, 18-24.Google Scholar

  • 19. Nisbet D.I., Mackay J.M., Smith W., Gray E.W.: Ultrastructure of sheep pulmonary adenomatosis (Jaagsiekte). J Pathol 1971,103, 157-162.Google Scholar

  • 20. Ortin A., Minguijon E., Dewar P., Garcia M., Ferrer L.M., Palmarini M., Gonzalez L., Sharp J.M., De Las Heras M.: Lack of a specific immune response against a recombinant capsid protein of jaagsiekte sheep retrovirus in sheep and goats naturally affected by enzootic nasal tumour or sheep pulmonary adenomatosis. Vet Immunol Immunopathol 1998, 61, 229-237.Google Scholar

  • 21. Palmarini M., Sharp J.M., De Las Heras M., Fan H.: Jaagsiekte sheep retrovirus is necessary and sufficient to induce a contagious lung cancer in sheep. J Virol 1999, 73, 6964-6972.Google Scholar

  • 22. Platt J.A., Kraipowich N., Villafane F.A., De Martini J.C.: Alveolar type II cells expressing jaagsiekte sheep retrovirus capsid protein and surfactant proteins are the predominant neoplastic cell type in ovine pulmonary adenocarcinoma. Vet Pathol 2002, 39, 341-352.Google Scholar

  • 23. Pusterla N., Braun U., Grest P., Ossent P.: Ein Fall von Lungenadenomatose bei einem Schaf: Diagnose durch Lungenbiopsie unter Ultraschallkontrolle. Tierärztl Umschau 1995, 50, 340-343.Google Scholar

  • 24. Rezazadeh F., Zarrini G.H., Cousens C., Attarilar N.: Prevalence of jaagsiekte sheep retrovirus infection in North-West Iran. Global Vet 2012, 9, 535-540.Google Scholar

  • 25. Rosadio R.H., Sharp J.M., Lairmore M.D., Dahlberg J.E., De Martini J.C.: Lesions and retroviruses associated with naturally occurring ovine pulmonary carcinoma (sheep pulmonary adenomatosis). Vet Pathol 1988, 25, 58-66.Google Scholar

  • 26. Salvatori D., Gonzalez L., Dewar P., Cousens C., De Las Heras M., Dalziel R.G., Sharp J.M.: Successful induction of ovine pulmonary adenocarcinoma in lambs of different ages and detection of viraemia during the preclinical period. J Gen Virol 2004, 85, 3319-3324.Google Scholar

  • 27. Scott P.R., Gessert M.E.: Ultrasonographic examination of the ovine thorax. Vet J 1998, 155, 305-310.Google Scholar

  • 28. Sharp J.M., De Martini J.: Natural history of JSRV in sheep. Curr Top Microbiol Immunol 2003, 275, 55-79.Google Scholar

  • 29. Sharp J.M., Herring A.: Sheep pulmonary adenomatosis: demonstration of a protein which crossreacts with the major core proteins of Mason-Pfizer monkey virus and mouse mammary tumour virus. J Gen Virol 1983, 64, 2323-2327.Google Scholar

  • 30. Wyn‐Jones G., Clarkson M.J.: Radiologic detection of ovine hydatidosis. Vet Radiol 1984, 25, 182-186.Google Scholar

About the article

Received: 2015-11-03

Accepted: 2016-02-15

Published Online: 2016-04-29

Published in Print: 2016-03-01

Citation Information: Journal of Veterinary Research, Volume 60, Issue 1, Pages 7–12, ISSN (Online) 2450-8608, DOI: https://doi.org/10.1515/jvetres-2016-0002.

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© 2016. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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