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Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

4 Issues per year


IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

CiteScore 2016: 0.46

SCImago Journal Rank (SJR) 2015: 0.230
Source Normalized Impact per Paper (SNIP) 2015: 0.383

Open Access
Online
ISSN
2450-8608
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Oxidative stress and antioxidant defence markers in muscle tissue of rainbow trout (Oncorhynchus mykiss) after vaccination against Yersinia ruckeri

Halyna Tkachenko
  • Department of Zoology and Animal Physiology, Institute of Biology and Environmental Protection, Pomeranian University in Slupsk, 76-200 Słupsk, Poland
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/ Joanna Grudniewska
  • Department of Salmonid Research, S. Sakowicz Inland Fisheries Institute, 83-330 Zukowo, Poland
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/ Agnieszka Pękala / Elżbieta Terech-Majewska
  • Department of Epizootiology, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland
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  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-04-29 | DOI: https://doi.org/10.1515/jvetres-2016-0005

Abstract

Introduction: The goal of this study was to assess the influence of vaccination against enteric redmouth disease on oxidative stress biomarkers and antioxidant defence in the muscle tissue of rainbow trout (Oncorhynchus mykiss Walbaum) vaccinated against Yersinia ruckeri in the first and second month after immunisation. Material and Methods: Healthy fish were vaccinated orally with inactivated whole cells of a virulent strain of Y. ruckeri. One and two months after immunisation the muscle samples were collected. Results: No significant difference was noted in lipid peroxidation level in either the first or second month after vaccination, while aldehydic and ketonic derivatives of oxidatively modified proteins (OMB) in the vaccinated group were significantly lower in the second month compared to those in the first month after vaccination (P < 0.05). The content of ketonic derivatives of OMB in muscles in the first month after immunisation was higher compared to untreated control. All these culminated in a depletion of glutathione peroxidase (GPx) activity and low level of total antioxidant capacity (TAC). Conclusion: Correlations between catalase activity and lipid peroxidation and TAC confirmed the pivotal role of catalase in antioxidant defence during immunisation. From a broader perspective, it is suggested that immunisation of fish with Yersinia vaccine is associated with induced free radical formation and oxidative stress. Free radicals would therefore be at least partially responsible for the induction of both humoral and cellular elements of the immunity and increased protective immunity against Y. ruckeri infection.

Keywords: rainbow trout; Oncorhynchus mykiss; Yersinia ruckeri; immunisation; oxidative stress; muscles

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About the article

Received: 2015-07-17

Accepted: 2016-02-03

Published Online: 2016-04-29

Published in Print: 2016-03-01


Citation Information: Journal of Veterinary Research, Volume 60, Issue 1, Pages 25–33, ISSN (Online) 2450-8608, DOI: https://doi.org/10.1515/jvetres-2016-0005.

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