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Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

4 Issues per year

IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

CiteScore 2016: 0.46

SCImago Journal Rank (SJR) 2015: 0.230
Source Normalized Impact per Paper (SNIP) 2015: 0.383

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Chinese herbal Jin-Ying-Tang attenuates the inflammatory response by inhibiting the activation of TLR4/MyD88/TRAF-6/NIK pathway at the mRNA level in LPS-stimulated mouse mammary epithelial cells

Qiong Yi
  • Biochemical Engineering Center of Guizhou Province
/ Xin Li
  • Biochemical Engineering Center of Guizhou Province
  • College of Animal Science, Guizhou University, 550025 Guiyang Guizhou, People’s Republic of China
/ Yuan-Fang Li
  • Biochemical Engineering Center of Guizhou Province
  • Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Klinikum der Universität München, Ludwig-Maximilians-Universität München, D-80336 München, Germany
/ Hang Yang
  • Biochemical Engineering Center of Guizhou Province
  • College of Animal Science, Guizhou University, 550025 Guiyang Guizhou, People’s Republic of China
/ Xiao-Yi Zhang
  • Biochemical Engineering Center of Guizhou Province
  • College of Animal Science, Guizhou University, 550025 Guiyang Guizhou, People’s Republic of China
/ Zhe Ma
  • Biochemical Engineering Center of Guizhou Province
  • Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Klinikum der Universität München, Ludwig-Maximilians-Universität München, D-80336 München, Germany
/ Lu Wang
  • Biochemical Engineering Center of Guizhou Province
  • Email:
Published Online: 2016-04-29 | DOI: https://doi.org/10.1515/jvetres-2016-0011


Introduction: The effects of Jin-Ying-Tang (JYT) on Toll-like Receptor 4 (TLR4) signalling transduction of lipopolysaccharide (LPS)-stimulated mouse mammary epithelial cells (MECs) in vitro were examined. Material and Methods: The cytotoxicity of JYT (0.06-62.50 mg/mL) on mouse MECs was determined by MTT assay. The MECs were co-cultured with LPS in the presence or absence of JYT (39.10 μg/mL, 391 μg/mL, 3910 μg/mL). The concentrations of interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) in the culture supernatants were detected by ELISA. The mRNA expression of TLR4 and downstream TLR4 signalling molecules such as myeloid differentiation factor 88 (MyD88), tumour necrosis factor receptor associated factor 6 (TRAF-6), inhibitor κB (IκB), and nuclear factor κB inducing kinase (NIK) were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Results: The results showed that the IC50 of JYT on MECs was 12.25 mg/mL and JYT could significantly decrease the concentrations of IL-6 and TNF-α in LPS-stimulated MECs (P < 0.05). The mRNA expression of TLR4, MyD88, TRAF-6, IκB, and NIK was also significantly decreased when the LPS-stimulated MECs were cocultured at appropriate concentrations of JYT (P < 0.05, P < 0.01). Conclusion: These observations indicate a potential mechanism through which JYT attenuates the systemic inflammatory response to LPS-stimulated mouse mammary epithelial cells by inhibiting the activation of TLR4/MyD88/ TRAF-6/NIK pathway at the mRNA level.

Keywords: mouse; JYT; mammary epithelial cells; cytokine; mRNA expression; signalling transduction


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About the article

Received: 2015-09-29

Accepted: 2016-02-19

Published Online: 2016-04-29

Published in Print: 2016-03-01

Citation Information: Journal of Veterinary Research, ISSN (Online) 2450-8608, DOI: https://doi.org/10.1515/jvetres-2016-0011. Export Citation

© 2016. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. (CC BY-NC-ND 4.0)

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