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Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

4 Issues per year

IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

CiteScore 2016: 0.46

SCImago Journal Rank (SJR) 2015: 0.230
Source Normalized Impact per Paper (SNIP) 2015: 0.383

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Comparison of laparoscopic and traditional abomasal cannulation in sheep

Shixia Zhang / Manliang Hao / Yuzhong Ma
Published Online: 2016-04-29 | DOI: https://doi.org/10.1515/jvetres-2016-0016


Introduction: The objective of this study was to describe a laparoscopic abomasal cannulation (LAC) technique, and compare the extent of the surgical trauma after LAC and open abomasal cannulation (OAC) by examining postoperative visual analog scale (VAS) pain scores and serum values of interleukin-6 and tumour necrosis factor-α in sheep. Material and Methods: Twelve healthy ewes, weighing 38-43 kg, were used. Three-portal laparoscopic techniques were used for LAC procedures. OAC was performed by a right flank laparotomy. Results: Abomasal cannulation was accomplished in all sheep without major intraoperative and postoperative complications. The abomasal contents were collected easily in both groups. Comparative studies found that open procedures exhibit a more pronounced short-term increase in cytokines and significantly higher VAS pain scores than the corresponding laparoscopic procedures. Conclusion: The laparoscopic technique proved to be less traumatic than the conventional open technique.

Keywords: sheep; abomasal cannulation; laparoscopy; interleukin-6; tumour necrosis factor-α


  • 1. Allen A.J., Park K.T., Barrington G.M., Lahmers K.K., Abdellrazeq G.S., Rihan H.M., Sreevatsan S., Davies C., Hamilton M.J., Davis W.C.: Experimental infection of a bovine model with human isolates of Mycobacterium avium subsp. paratuberculosis. Vet Immunol Immunopathol 2011, 141, 258-266.Web of ScienceGoogle Scholar

  • 2. Baigrie R.J., Lamont P.M., Kwiatkowski D., Dallman M.J., Morris P.J.: Systemic cytokine response after major surgery. Br J Surg 1992, 79, 757-760.Google Scholar

  • 3. Bingener J., Krishnegowda N.K., Michalek J.E.: Immunologic parameters during NOTES compared with laparoscopy in a randomized blinded porcine trial. Surg Endosc 2009, 23, 178-181.Web of ScienceGoogle Scholar

  • 4. Cambridge A.J., Tobias K.M., Newberry R.C., Sarkar D.K.: Subjective and objective measurements of postoperative pain in cats. J Am Vet Med Assoc 2000, 217, 685-690.Google Scholar

  • 5. Charles B.G., Filippich L., Pass M.A.: Pharmacokinetics and absolute bioavailability of cyclosporine following intravenous and abomasal administration to sheep. J Pharm Pharmacol 1993, 45, 821-824.Google Scholar

  • 6. Choileain N.N., Redmond P.: Cell response to surgery. Arch Surg 2006, 141, 1132-1140.Google Scholar

  • 7. Cruickshank A.M., Fraser W.D., Burns H.J., Van Damme J., Shenkin A.: Response of serum interleukin 6 in patients undergoing elective surgery of varying severity. Clin Sci 1990, 79, 161-165.Google Scholar

  • 8. Davidson E.B., Moll H.D., Payton M.E.: Comparison of laparoscopic ovariohysterectomy and ovariohysterectomy in dogs. Vet Surg 2004, 33, 62-69.Google Scholar

  • 9. Decker D., Lindemann C., Low A., Bidlingmaier F., Hirner A., von Ruecker A.: Changes in the cytokine concentration (IL-6, IL-8, IL-1ra) and their cellular expression of membrane molecules (CD25, CD30, HLA-DR) after surgical trauma. Zentralbl Chir 1997, 122, 157-163.Google Scholar

  • 10. Dunay M.P., Németh T., Makra Z., Izing S., Bodó G.: Laparoscopic cryptorchidectomy and ovariectomy in standing horses using the EnSeal® tissue-sealing device. Acta Vet Hung 2012, 60, 41-53.Google Scholar

  • 11. Dunker M.S., Ten Hove T., Bemelman W.A., Slors J.F., Gouma D.J., Van Deventer S.J.: Interleukin 6, C-reactive protein, and expression of human leukocyte antigen-DR on peripheral blood mononuclear cells in patients after laparoscopic vs. conventional bowel resection. Dis Colon Rectum 2003, 46, 1238-1244.Google Scholar

  • 12. Firth A.M., Haldane S.L.: Development of a scale to evaluate postoperative pain in dogs. J Am Vet Med Assoc 1999, 214, 651-659.Google Scholar

  • 13. Hertzberq H., Guscetti F., Bödeker D., Pospischil A., Eckert J.: Access to the abomasum of sheep via a rumen fistula for followup studies during trichostrongylid infections. Zentralbl Veterinarmed B 1994, 41, 237-248.Google Scholar

  • 14. Holtenius K., Sternbauer K., Holtenius P.: The effect of the plasma glucose level on the abomasal function in dairy cows. J Anim Sci 2000, 78, 1930-1935.Google Scholar

  • 15. Johnson R.R.: Techniques and procedures for in vitro and in vivo rumen studies. J Anim Sci 1966, 25, 855-875.Google Scholar

  • 16. Knight J.S., Baird D.B., Hein W.R., Pernthaner A.: The gastrointestinal nematode Trichostrongylus colubriformis downregulates immune gene expression in migratory cells in afferent lymph. BMC Immunol 2010, 11, 51-62.Google Scholar

  • 17. Komarek R.J.: Rumen and abomasal cannulation of sheep with specially designed cannulae and a cannula insertion instrument. J Anim Sci 1981, 53, 790-795.Google Scholar

  • 18. Kristensen N.B., Engbaek M., Vestergaard M., Harmon D.L.: Technical note: ruminal cannulation technique in young Holstein calves: effects of cannulation on feed intake, body weight gain, and ruminal development at six weeks of age. J Dairy Sci 2010, 93, 737-742.Web of ScienceGoogle Scholar

  • 19. Lennard T.W., Shenton B.K., Borzotta A., Donnelly P.K., White M., Gerrie L.M., Proud G., Taylor R.M.: The influence of surgical operations on components of the human immune system. Br J Surg 1985, 72, 771-776.Google Scholar

  • 20. Leung K.L, Lai P.B., Ho R.L., Meng W.C., Yiu R.Y., Lee J.F., Lau W.Y.: Systemic cytokine response after laparoscopicassisted resection of rectosigmoid carcinoma: A prospective randomized trial. Ann Surg 2000, 231, 506-511.Google Scholar

  • 21. Mathon D.H., Dossin O., Palierne S., Cremoux M., Rodriguez H., Meynaud-Collard P., Steiner J.M., Suchodolski J.S., Lefebvre H.P., Autefage A.: A laparoscopic-sutured gastropexy technique in dogs: mechanical and functional evaluation. Vet Surg 2009, 38, 967-974.Web of ScienceGoogle Scholar

  • 22. Mathon D.H., Palierne S., Meynaud-Collard P., Layssol- Lamour C., Dulaurent-Ferrieres A., Colson A., Lacroix M., Bousquet-Melou A., Delverdier M., Autefage A.: Laparoscopicassisted colopexy and sterilization in male dogs: short-term results and physiologic consequences. Vet Surg 2011, 40, 500-508.Google Scholar

  • 23. Palmer J.E., Whitlock R.H.: Bleeding abomasal ulcers in adult dairy cows. J Am Vet Med Assoc 1983, 183, 448-451.Google Scholar

  • 24. Pearson E.G., Guard C.L., Smith D.F.: A method for obtaining abomasal fluid via a rumen fistula. Cornell Vet 1981, 71, 183-187.Google Scholar

  • 25. Rawlings C.: Endoscopic removal of urinary calculi. Compend Contin Educ Vet 2009, 31, 476-484.Google Scholar

  • 26. Ross J.G., Purcell D.A., Todd J.R.: Experimental infection of calves with Trichostrongylus axei investigations using abomasal cannulae. Res Vet Sci 1969, 10, 46-56.Google Scholar

  • 27. Rupp G.P., Kreikemeier K.K., Perino L.J., Ross G.S.: Measurement of volatile fatty acid disappearance and fluid flux across the abomasum of cattle, using an improved omasal cannulation. Am J Vet Res 1994, 55, 522-529.Google Scholar

  • 28. Vlaminck K., van den Hende C., Oyaert W., Muylle E.: Studies on abomasal emptying in cattle. II. Effect of infusions in duodenum and abomasum on electromyographic complexes, pressure changes and emptying of the abomasum. Zentralbl Veterinarmed A 1984, 31, 676-682.Google Scholar

  • 29. Wilson E.R., Henderson R.A., Montgomery R.D., Kincaid S.A., Wright J.C., Hanson R.R.: A comparison of laparoscopic and belt-loop gastropexy in dogs. Vet Surg 1996, 25, 221-227.Google Scholar

  • 30. Zhang S.X., Wang H.B., Zhang J.T., Zhang N., Pan L.: Laparoscopic colopexy in dogs. J Vet Med Sci 2013, 75, 1161-1166.Web of ScienceGoogle Scholar

About the article

Received: 2015-10-25

Accepted: 2016-02-01

Published Online: 2016-04-29

Published in Print: 2016-03-01

Citation Information: Journal of Veterinary Research, Volume 60, Issue 1, Pages 113–117, ISSN (Online) 2450-8608, DOI: https://doi.org/10.1515/jvetres-2016-0016.

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© 2016. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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