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Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

4 Issues per year


IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

CiteScore 2016: 0.46

SCImago Journal Rank (SJR) 2015: 0.230
Source Normalized Impact per Paper (SNIP) 2015: 0.383

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2450-8608
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Effect of Enterococcus faecium EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with Salmonella Enteritidis

Zuzana Ševčíková
  • Institute of Pathological Anatomy, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia Republic
  • Email:
/ Ján Blanár
  • Imuna s.r.o., 082 22 Šarišské Michaľany, Slovakia Republic
/ Andrea Lauková
  • Institute of Animal Physiology, Slovak Academy of Science, 040 01 Košice, Slovakia Republic
/ Viera Revajová
  • Institute of Pathological Anatomy, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia Republic
/ Viola Strompfová
  • Institute of Animal Physiology, Slovak Academy of Science, 040 01 Košice, Slovakia Republic
/ Mikuláš Levkut
  • Institute of Pathological Anatomy, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia Republic
Published Online: 2016-09-23 | DOI: https://doi.org/10.1515/jvetres-2016-0040

Abstract

Introduction: The present study aimed to investigate the effect of Enterococcus faecium EF55 on chickens, as well as its influence on proliferative activity of epithelial intestinal cells after infection with Salmonella enterica serovar Enteritidis phage type 4 (SE PT4). Moreover, the length and area of duodenal and jejunal villi of the birds were examined.

Material and Methods: A pool of 80 birds was divided randomly into four groups. Probiotic group (EF) and Salmonella + probiotic group (EFSE) received E. faecium EF55 (109 CFU – 3 g per group/day) during 22 d. Salmonella group (SE) and EFSE group were infected with Salmonella Enteritidis PT4 (108 CFU in 0.2 mL PBS) in a single dose per os on day four of the experiment, whereas control birds (C group) received only 0.2 mL of PBS on that day. Samples were taken on the 4th and 18th day post infection.

Results: Supplementation of feed with E. faecium EF 55 confirmed its selective antibacterial activity against SE PT4. The chickens infected with SE PT4 and fed E. faecium EF55 supplemented diet showed increased proliferative activity of enterocytes in the jejunum in both samplings. Applied probiotic strain demonstrated positive impact on intestinal morphometry in the jejunum of both non-infected groups and in Salmonella-infected chickens. In the latter group, the beneficial effect of E. faecium EF 55 was manifested by more efficient tissue turnover in the jejunum.

Keywords: chickens; probiotic; Salmonella; intestinal morphometry; proliferative activity of enterocytes

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About the article

Received: 2016-04-07

Accepted: 2016-09-06

Published Online: 2016-09-23

Published in Print: 2016-09-01


Citation Information: Journal of Veterinary Research, ISSN (Online) 2450-8608, DOI: https://doi.org/10.1515/jvetres-2016-0040.

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© 2016 Zuzana Ševčíková et al., published by De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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