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Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

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IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

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2450-8608
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Evaluation of susceptibility to antimycobacterial drugs in Mycobacterium tuberculosis complex strains isolated from cattle in Poland

Monika Krajewska-Wędzina
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
  • Email:
/ Anna Zabost
  • Department of Microbiology, National Tuberculosis and Lung Diseases Institute in Warsaw, 01-138 Warsaw, Poland
/ Ewa Augustynowicz-Kopeć
  • Department of Microbiology, National Tuberculosis and Lung Diseases Institute in Warsaw, 01-138 Warsaw, Poland
/ Marcin Weiner
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
  • Pope John Paul II State School of Higher Education in Biala Podlaska, 21-500 Biała Podlaska, Poland
/ Krzysztof Szulowski
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
Published Online: 2017-04-04 | DOI: https://doi.org/10.1515/jvetres-2017-0003

Abstract

Introduction: Tuberculosis is a highly infectious disease affecting humans and animals. It is caused by the Mycobacterium tuberculosis complex (MTBC) – Mycobacterium bovis and Mycobacterium caprae, which are aetiological factors of bovine tuberculosis (bTB). In Poland, the bTB eradication programme exists. Animals diagnosed with tuberculosis are in the majority of cases not treated, but removed from their herd and then sanitary slaughtered.

Material and Methods: In total, 134 MTBC strains isolated from cattle in Poland were subjected to microbiological analysis. The resistance phenotype was tested for first-line antimycobacterial drugs used in tuberculosis treatment in humans: streptomycin, isoniazid, rifampicin, ethambutol, and pyrazinamide. The strains were isolated from tissues collected post mortem, so the test for drug resistance fulfilled only epidemiological criterion.

Results: The analysis of drug-resistance of MTBC strains revealed that strains classified as M. bovis were susceptible to 4 antimycobacterial drugs: isoniazid, rifampicin, streptomycin, and ethambutol, and resistant to pyrazynamide. The strains classified as M. caprae were sensitive to all tested drugs.

Conclusion: The results indicate that despite enormously dynamic changes in mycobacterial phenotype, Polish strains of MTBC isolated from cattle have not acquired environmental resistance. The strains classified as M. bovis are characterised by natural resistance to pyrazinamide, which is typical for this species.

Keywords: cattle; bovine tuberculosis; Mycobacterium tuberculosis complex; antimycobacterial drugs; Poland

References

  • 1. Aranaz A., Cousins D., Mateos A., Domínguez L.: Elevation of Mycobacterium tuberculosis subsp. caprae Aranaz et al. 1999 to species rank as Mycobacterium caprae comb. nov., sp. nov. Int J Syst Evol Microbiol 2003, 53, 1785–1789.

  • 2. Bailey S.S., Crawshaw T.R., Smith N.H., Palgrave C.J.: Mycobacterium bovis infection in domestic pigs in Great Britain. Vet J 2013, 198, 391–397.

  • 3. Beltrán-Beck B., Ballestros C., Vicente J., de la Fuente J., Gortázar C.: Progress in oral vaccination against tuberculosis in its main wildlife resevoir in Iberia, the Eurasian wild boar. Vet Med Int 2012, doi: 10.1155/2012/978501. [Crossref]

  • 4. Bobadilla-del Valle M., Torres-González P., Cervera-Hernández M.E., Martínez-Gamboa A., Crabtree-Ramirez B., Chávez-Mazari B., Ortiz-Conchi N., Rodríguez-Cruz L., Cervantes-Sánchez A., Gudiño-Enríquez T., Cinta-Severo C., Sifuentes-Osornio J., Ponce de León A.: Trends of Mycobacterium bovis isolation and first-line anti-tuberculosis drug susceptibility profile: A fifteen-year laboratory-based surveillance. PLoS Negl Trop Dis 2015, doi: 10.1371/journal.pntd.0004124. [Crossref]

  • 5. Campbell T.A., Long D.B., Bazan L.R., Thomsen B.V., Robbe-Austerman S., Davey R.B., Soliz L.A., Swafford S.R., VerCauteren K.C.: Absence of Mycobacterium bovis in feral swine (Sus scrofa) from the southern Texas border region. J Wildl Dis 2011, 47, 974–978.

  • 6. Cavirani S., Fanti F., Benecchi M., Calderaro A., Taddei S., Arcangeletti C., Medici M.C., Dettori G., Chezzi C.: Evaluation of susceptibility of Mycobacterium bovis to antituberculous drugs by radiometric BACTEC 460TB system. New Microbiol 2003, 26, 181–186.

  • 7. Chiu Chang K., Wai Yew W., Chiu R., Chan Y.: Rapid assays for fluoroquinolone resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis. J Antimicrob Chemother 2010, 65, 1551–1561.

  • 8. Commission Decision 2009/342/EC as regards the declaration that certain administrative regions of Poland are officially free of zoonotic – bovine – leukosis and that Poland and Slovenia are officially free of bovine tuberculosis. Oj L 104, 24. 4. 2009, 51–56.

  • 9. Corner L.A.: The role of wild animal populations in the epidemiology of tuberculosis in domestic animals: how to assess the risk. Vet Microbiol 2006, 112, 303–312.

  • 10. Council Directive 64/432/EWG of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine. OJ L 121, 29.7.1964, 1977–2012.

  • 11. Crawshaw T., Daniel R., Clifton-Hadley R., Clark J., Evans H., Rolfe S., de la Rua-Domenech R.: TB in goats caused by Mycobacterium bovis. Vet Rec 2008, 163, 127.

  • 12. Daly M., Diegel K.L., Fitzgerald S.D., Schooley A., Berry D.E., Kaneene J.B.: Patterns of antimicrobial susceptibility in Michigan wildlife and bovine isolates of Mycobacterium bovis. J Vet Diagn Invest 2006, 18, 401–404. [Crossref]

  • 13. Fitzgerald S.D., Schooley A.M., Berry D.E., Kaneene J.B.: Antimicrobial susceptibility testing of Mycobacterium bovis isolates from Michigan white-tailed deer during the 2009 hunting season. Vet Med Int 2011, doi:10.4061/2011/903683. [Crossref]

  • 14. Gallagher J., Clifton-Hadley R.S.: Tuberculosis in badgers; a review of the disease and its significance for other animals. Res Vet Sci 2000, 69, 203–217.

  • 15. Karlson A.G., Lessel E.F.: Mycobacterium bovis nom. Nov Int J Syst Bacteriol 1970, 20, 273–282. [Crossref]

  • 16. Kozińska M., Zientek J., Augustynowicz-Kopeć E., Zwolska Z., Kozielski J.: Transmission of tuberculosis among people living in the border areas of Poland, the Czech Republic, and Slovakia. Pol Arch Med Wewn 2016, 126, 32–40.

  • 17. Krajewska M., Kozińska M., Kubajka M., Weiner M., Augustynowicz-Kopeć E., Bełkot Z., Lipiec M., Szulowski K.: Tuberculosis in humans and in animals – current epidemiological data. Życie Wet 2015, 90, 647–651.

  • 18. Krajewska M., Kozińska M., Zwolska Z., Lipiec M., Augustynowicz-Kopeć E., Szulowski K.: Human as a source of tuberculosis for cattle. First evidence of transmission in Poland. Vet Microbiol 2012, 159, 269–271. [Web of Science]

  • 19. Krajewska M., Napiórkowska A., Augustynowicz-Kopeć E.: Pyrazinamide resistance among Mycobacterium bovis strains isolated from animals in Poland. Życie Wet 2013, 88, 575–577.

  • 20. Krajewska M., Załuski M.: Tuberculosis in giraffe – attempt to treatment. Abstract of 2nd Conference Pharmacology Department of University of Life Sciences in Lublin: Pharmacological and environmental aspects of rational therapy. Krynica Górska, Poland, 2012, p.20.

  • 21. Kruczak K., Niżankowska-Mogilnicka E.: Multidrug resistance tuberculosis – current problems. Pneumol Alergol Pol 2009, 77, 276–283.

  • 22. Michałowska-Mitczuk D. Pharmacotherapy of tuberculosis. Post Farmakoterapii 2009, 65, 51–58.

  • 23. Monies R.J., Cranwell M.P., Palmer N., Inwald J., Hewinson R.G., Rule B.: Bovine tuberculosis in domestic cats. Vet Rec 2000, 146, 407–408.

  • 24. Monreal L., Segura D., Segalés J., Garrido J.M., Prades M.: Diagnosis of Mycobacterium bovis infection in a mare. Vet Rec 2001, 149, 712–714.

  • 25. Muñoz Mendoza M., Juan L.D., Menéndez S., Ocampo A., Mourelo J., Sáez J.L., Domínguez L., Gortázar C., García Marín J.F., Balseiro A.: Tuberculosis due to Mycobacterium bovis and Mycobacterium caprae in sheep. Vet J 2012, 191, 267–269.

  • 26. Napiórkowska A., Zwolska Z., Augustynowicz-Kopeć E.: Resistance to pyrazinamide among Mycobacterium tuberculosis strains isolated from patients from the Mazowian Voivodeship in 2008 – 2010 years. Post Nauk Med 2011, 24, 819–823.

  • 27. Olesiejuk R., Maciak L., Milanowski J.: Meaning of the radiometric method Bactec 460 TB for the application of antituberculosis therapy. Pneumol Alergol Pol 2002, 70, 5–11.

  • 28. Osek J., Wieczorek K.: Prevalence of zoonoses in animals and zoonotic agents in food in Europe in 2013. Życie Wet 2015, 90, 210–216.

  • 29. Rodriguez-Campos S., Smith N.H., Boniotti M.B., Aranaz A.: Overview and phylogeny of Mycobacterium tuberculosis complex organisms: implication for diagnostics and legislation of bovine tuberculosis. Res Vet Sci 2014, 97, 5–19.

  • 30. Safinowska A., Walkiewicz R., Nejman-Gryz P., Grubek-Jaworska H.: Two selected commercially based nucleic acid amplification tests for the diagnosis of tuberculosis. Pneumonol Alergol Pol 2012, 80, 6–12.

  • 31. Shrikrishana D., de la Rua-Domenech R., Smith N.H., Colloff A., Coutts I.: Human and canine pulmonary Mycobacterium bovis infections in the same household: re-emergence of an old zoonotic threat? Thorax 2009, 64, 89–91.

  • 32. van der Burgt G.M., Crawshaw T., Foster A.P., Denny D.J., Schock A.: Mycobacterium bovis infection in dogs. Vet Rec 2009, 165, 634.

  • 33. Vazquez-Chacon C.A., Martínez-Guarneros A., Couvin D., González-Y-Merchand J.A., Rivera-Gutierrez S., Escobar-Gutierrez A., De-la-Cruz López J.J., Gomez-Bustamante A., Gonzalez-Macal G.A., Gonçalves Rossi L.M., Muñiz-Salazar R., Rastogi N., Vaughan G.: Human multidrug-resistant Mycobacterium bovis infection in Mexico. Tuberculosis (Edinb) 2015, doi: 10.1016/j.tube.2015.07.010. [Crossref]

  • 34. World Health Organization. TB and HIV Programme. http://www.who.int/tb/areas-of-work/tb-hiv/en/

About the article

Received: 2016-12-07

Accepted: 2017-03-08

Published Online: 2017-04-04

Published in Print: 2017-03-01



Citation Information: Journal of Veterinary Research, ISSN (Online) 2450-8608, DOI: https://doi.org/10.1515/jvetres-2017-0003. Export Citation

© 2017 Monika Krajewska-Wędzina et al., published by De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

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