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Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

4 Issues per year


IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

CiteScore 2016: 0.46

SCImago Journal Rank (SJR) 2015: 0.230
Source Normalized Impact per Paper (SNIP) 2015: 0.383

Open Access
Online
ISSN
2450-8608
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Toxinotyping and antimicrobial resistance of Clostridium perfringens isolated from processed chicken meat products

Dalia Hamza
  • Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Giza 11221, Egypt
  • Email:
/ Sohad Dorgham
  • Department of Microbiology and Immunology, National Research Centre, Giza 12622, Egypt
/ Ashraf Hakim
  • Department of Microbiology and Immunology, National Research Centre, Giza 12622, Egypt
Published Online: 2017-04-04 | DOI: https://doi.org/10.1515/jvetres-2017-0007

Abstract

Introduction: The toxinotyping and antimicrobial susceptibility of Clostridium perfringens strains isolated from processed chicken meat were determined.

Material and Methods: Two hundred processed chicken meat samples from luncheon meats, nuggets, burgers, and sausages were screened for Clostridium perfringens by multiplex PCR assay for the presence of alpha (cpa), beta (cpb), epsilon (etx), iota (ia), and enterotoxin toxin (cpe) genes. The C. perfringens isolates were examined in vitro against eight antibiotics (streptomycin, amoxicillin, ampicillin, ciprofloxacin, lincomycin, cefotaxime, rifampicin, and trimethoprim-sulfamethoxazole)

Results: An overall of 32 C. perfringens strains (16%) were isolated from 200 processed chicken meat samples tested. The prevalence of C. perfringens was significantly dependent on the type of toxin genes detected (P = 0.0), being the highest in sausages (32%), followed by luncheon meats (24%), burgers (6%), and nuggets (2%). C. perfringens type A was the most frequently present toxinotype (24/32; 75%), followed by type D (21.9 %) and type E (3.1%). Of the 32 C. perfringens strains tested, only 9 (28%) were enterotoxin gene carriers, with most representing type A (n = 6). C. perfringens strains differed in their resistance/susceptibility to commonly used antibiotics. Most of the strains tested were sensitive to ampicillin (97%) and amoxicillin (94%), with 100% of the strains being resistant to streptomycin and lincomycin. It is noteworthy that the nine isolates with enterotoxigenic potential had a higher resistance than the non-enterotoxigenic ones.

Conclusion: The considerably high C. perfringens isolation rates from processed chicken meat samples and resistance to some of the commonly used antibiotics indicate a potential public health risk. Recent information about the isolation of enterotoxigenic C. perfringens type E from chicken sausage has been reported.

Keywords: processed chicken meat; Clostridium perfringens; enterotoxin; antimicrobial resistance

References

  • 1. Abd-El Gwad A.M., Abd El-Kader H.A.: The occurrence of Clostridium perfringens in the intestine of broiler chickens Assiut governorate. Ass Univ Bull Environ Res 2001, 4, 13–22.Google Scholar

  • 2. Abd El–Hamid H.S., Ellakany H.F., Aboelmagd B.A., Elbestawy A.R., Bedawy Sh.: Clinical and laboratory studies on chicken isolates of Clostridium perfringens in El–Behera, Egypt. J World's Poult Res 2015, 5, 21–28.Google Scholar

  • 3. Agunos A., Léger D., Carson C.: Review of antimicrobial therapy of selected bacterial diseases in broiler chickens in Canada. Can Vet J 2012, 53, 1289–300.Google Scholar

  • 4. Ata N., Khairy E.A., Dorgham S.M., Zaki M.S.: Clostridium perfringens disease. Life Sci J 2013, 10, 1599–1602.Google Scholar

  • 5. Bos J., Smithee L., McClane B., Distefano R.F., Uzal F.: Fatal necrotizing colitis following a foodborne outbreak of enterotoxigenic Clostridium perfringens type A infection. Clin Infect Dis 2005, 40, 78–83.CrossrefGoogle Scholar

  • 6. Brynestad S., Granum P.E.: Clostridium perfringens and foodborne infections. Int J Food Microbiol 2002, 74, 195–202.CrossrefGoogle Scholar

  • 7. Cakmak O., Ormanc F.S.D., Tayfur M., Erol I.: Presence and contamination level of Clostridium perfringens in raw frozen ground poultry and poultry burgers. Turk J Vet Anim Sci 2006, 30, 101–105.Google Scholar

  • 8. CLSI: Performance standards for antimicrobial susceptibility testing, Twenty-Fourth Informational supplement. 2012, M100–S24. Villanova, USA.Google Scholar

  • 9. Collee J.G., Fraser A.G., Marmion B.P., Simmons A.: Practical medical microbiology. 14th education, Churchill Living Stone, New York 1996.Google Scholar

  • 10. Craven S.E., Cox N.A., Bailey J.S., Cosby D.E.: Incidence and tracking of Clostridium perfringens through an integrated broiler chicken operation. Avian Dis 2003, 47, 707–711.CrossrefGoogle Scholar

  • 11. Craven S.E., Cox N.A., Stern N.J., Mauldin J.M.: Prevalence of Clostridium perfringens in commercial broiler hatcheries. Avian Dis 2001, 45, 1050–1053.CrossrefGoogle Scholar

  • 12. Freedman C.J., Archana S., Bruce A., McClane B.A.: Clostridium perfringens enterotoxin: action, genetics, and translational applications. Toxins 2016, 8, 73.CrossrefGoogle Scholar

  • 13. Guran H.S., Oksuztepe G.: Detection and typing of Clostridium perfringens from retail chicken meat parts. Lett Appl Microbiol 2013, 57, 77–82.CrossrefGoogle Scholar

  • 14. Hamza D.A., Ghoneim N.H.: Epidemiological studies on Clostridium perfringens food poisoning, Scholar press. Omniscriptum Marketing DEU, Germany 2015.Google Scholar

  • 15. Hook D., Jalaludin B., Fitzsimmons G.: Clostridium perfringens food-borne-outbreak: an epidemiological investigation. Aust NZ J Public Health 1996, 20, 119–122.Google Scholar

  • 16. Khan M., Nazir J., Anjum A.A., Ahmad M.U., Nawaz M.: Toxinotyping and antimicrobial susceptibility of enterotoxigenic Clostridium perfringens isolates from mutton, beef and chicken meat. J Food Sci Technol 2015, 52, 5323–5328.CrossrefGoogle Scholar

  • 17. Martel A., Devriese L.A., Cauwerts K., De-Gussem K., Decostere A.: Susceptibility of Clostridium perfringens isolates from broiler chickens to antibiotics and anti-coccidials. Avian Pathol 2004, 31, 3–7.CrossrefGoogle Scholar

  • 18. McClane B.A.: Clostridium perfringens, In: Food Microbiology, edited by Doyle M.P., Beuchat L.R., ASM press, Washington 2007, pp. 423–444.Google Scholar

  • 19. Miki Y., Miyamoto K., Kaneko-Hirano I., Fujiuchi K., Akimoto S.: Prevalence and characterization of enterotoxin gene carrying Clostridium perfringens from retail meat products in Japan. Appl Environ Microbiol 2008, 74, 5366–5372.CrossrefGoogle Scholar

  • 20. Miwa N., Nishina T., Kubo S., Atsumi M, Honda H.: Amount of enterotoxigenic Clostridium perfringens in meat detected by nested PCR. Int J Food Microbiol 1998, 42, 195–200.CrossrefGoogle Scholar

  • 21. Miyamoto K., Li J., McClane B.A.: Enterotoxigenic Clostridium perfringens: detection and identification. Microbes Environ 2012, 27, 343–349.CrossrefGoogle Scholar

  • 22. Murray P.R., Baron E.J.O., Pfaller M.A.J., Orgensen J.H., Yolken R.H.: Clostridium, Manual of Clinical Microbiology, Vol. 1, ASM Press, Washington 2003, pp. 940–966.Google Scholar

  • 23. Nasr E.M., Shehta A.A., Amer A.H.: Enterotoxigenicity and typing of Clostridium perfringens isolates from some poultry products in Egypt. J Appl Sci Res 2007, 3, 1804–1808.Google Scholar

  • 24. Nowell V.J., Poppe C., Parreira V.R., Jiang Y.F., Reid-Smith R.: Clostridium perfringens in retail chicken. Anaerobe 2010, 16, 314–315.CrossrefGoogle Scholar

  • 25. Okolocha E.C., Ellerbroek L.: The influence of acid and alkaline treatments on pathogens and the shelf life of poultry meat. Food Control 2005, 16, 217–225.CrossrefGoogle Scholar

  • 26. Osman K.M., Elhariri M.: Antibiotic resistance of Clostridium perfringens isolates from broiler chickens in Egypt. Rev Sci Tech Off Int Epiz 2013, 32, 841–850.CrossrefGoogle Scholar

  • 27. Silva R.O.S., Francisco C.F.J., Marcus V.R.M., Carlos A.O.J., Nelson R.M.: Genotyping and antimicrobial susceptibility of Clostridium perfringens isolated from Tinamidae, Cracidae and Ramphastidae species. Brazil Cienc Rural 2014, 44, 486–491.CrossrefGoogle Scholar

  • 28. van Asten A.J., van der Wiel C.W., Nikolaou G., Houwers D.J., Grone A.A.: Multiplex PCR for toxin typing of C. perfringens. Vet Microbiol 2009, 136, 411–412.CrossrefGoogle Scholar

  • 29. Voidarou C., Vassos D., Rozos G., Alexopoulos A., Plessas A.: Microbial challenges of poultry meat production. Anaerobe 2011, 17, 341–343.CrossrefWeb of ScienceGoogle Scholar

About the article

Received: 2016-09-26

Accepted: 2017-03-08

Published Online: 2017-04-04

Published in Print: 2017-03-01


Citation Information: Journal of Veterinary Research, ISSN (Online) 2450-8608, DOI: https://doi.org/10.1515/jvetres-2017-0007.

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© 2017 Dalia Hamza et al., published by De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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