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Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

4 Issues per year


IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

CiteScore 2016: 0.46

SCImago Journal Rank (SJR) 2015: 0.230
Source Normalized Impact per Paper (SNIP) 2015: 0.383

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ISSN
2450-8608
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Metabolic activity of boar semen stored in different extenders supplemented with ostrich egg yolk lipoproteins

Anna Dziekońska
  • Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
  • Email:
/ Marek Kinder
  • Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
/ Leyland Fraser
  • Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
/ Jerzy Strzeżek
  • Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
/ Władysław Kordan
  • Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
Published Online: 2017-04-04 | DOI: https://doi.org/10.1515/jvetres-2017-0016

Abstract

Introduction: The aim of this study was to evaluate the effect of lipoprotein fraction isolated from ostrich egg yolk (LPFo) on the metabolic activity of boar spermatozoa following liquid semen storage in different extenders and temperatures.

Material and Methods: Boar ejaculates were extended in Androhep, Beltsville thawing solution (BTS), and Martín-Rillo and Alias (MR-A) without (control) or with the addition of LPFo and stored for three days at either 5°C or 16°C. The analysed sperm parameters included total motility (TMOT), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), oxygen consumption, and adenosine triphosphate (ATP) production.

Results: The sperm metabolic activity seemed to be higher in the LPFo-based extenders following storage for three days, irrespective of the storage temperature. Compared with the LPFo-free extenders, significantly higher (P < 0.05) sperm PMI and MMP were observed in BTS and MR-A extenders supplemented with LPFo during storage for three days at 5°C. Spermatozoa stored in the BTS-LPFo extender exhibited higher (P < 0.05) TMOT and oxygen consumption, whereas higher (P < 0.05) PMI was observed in spermatozoa stored in Androhep-LPFo and MR-A-LPFo for three days at 16°C. No significant differences (P > 0.05) in ATP content were observed between the LPFo-free and LPFo-based extenders during storage.

Conclusions: Supplementation of LPFo to semen extenders had varying effects on the metabolic activity of boar spermatozoa stored at different temperatures. It can be suggested that the interactions of various components of the extenders and seminal plasma with LPFo exert beneficial effects on the sperm metabolic activity during liquid storage of boar semen.

Keywords: boar; sperm; extender; lipoprotein; metabolism

References

  • 1. Becherer D., Batz J., Gugelmann R., Ulbrich S.E., Bollwein H., Janett F.: Long term storage of liquid preserved boar semen: A comparative study using 5 different commercial extenders. Reprod Domest Anim 2014, 49, 10, doi: 10.1111/rda.12292.CrossrefGoogle Scholar

  • 2. Bergeron A., Manjunath P.: New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Mol Reprod Dev 2006, 73, 1338–1344.Google Scholar

  • 3. Demianowicz W., Strzeżek J.: The effect of lipoprotein fraction from egg yolk on some of the biological properties of boar spermatozoa during storage of the semen in liquid state. Reprod Domest Anim 1996, 31, 279–280.CrossrefGoogle Scholar

  • 4. Dziekońska A., Fraser L., Kinder M., Strzeżek J.: Energy metabolism in boar spermatozoa stored in different extenders at 5 degrees C and 16 degrees C. Reprod Domest Anim 2007, 42, 90, doi: 10.1111/j.1439-0531.2007.00910.x.CrossrefGoogle Scholar

  • 5. Dziekońska A., Fraser L., Strzeżek J.: Effect of different storage temperatures on the metabolic activity of spermatozoa following liquid storage of boar semen. J Anim Feed Sci 2009, 18, 638–649.CrossrefGoogle Scholar

  • 6. Dziekońska A., Strzezek J.: Boar variability affects sperm metabolism activity in liquid stored semen at 5 degrees C. Pol J Vet Sci 2011, 14, 21–27.Web of ScienceGoogle Scholar

  • 7. Dziekońska A., Fraser L., Majewska A., Lecewicz, M., Zasiadczyk Ł., Kordan W.: Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17 degrees C. Pol J Vet Sci 2013, 16, 517–525.Web of ScienceGoogle Scholar

  • 8. Flowers W.L.: Semen quality assurance. 49th Ann. North Carolina Pork Con., Greenville, USA. https://www.ncsu.edu/project/swine_extension/ncporkconf/2005/sessions/flowers.htm.

  • 9. Fraser L., Dziekońska A., Strzeżek R., Strzeżek J.: Dialysis of boar semen prior to freezing–thawing: Its effects on post-thaw sperm characteristics. Theriogenology 2007, 67, 994–1003.Web of ScienceGoogle Scholar

  • 10. Fraser L., Gorszczaruk K., Strzeżek J.: Relationship between motility and membrane integrity of boar spermatozoa in media varying in osmolality. Reprod Domest Anim 2001, 36, 325–329.CrossrefGoogle Scholar

  • 11. Fraser L., Strzeżek R., Strzeżek J.: Fertilizing capacity of boar semen frozen in an extender supplemented with ostrich egg yolk lipoprotein fractions – a preliminary study. Pol J Vet Sci 2007, 10, 131–135.Google Scholar

  • 12. Fraser L., Zasiadczyk Ł., Strzeżek J.: Interactions of egg yolk lipoprotein fraction with boar spermatozoa assessed with a fluorescent membrane probe. Folia Histochem Cytochem 2010, 48, 292–298.Google Scholar

  • 13. Gadea J.: Semen extenders used in the artificial insemination of swine. Spanish J Agric Res 2003, 1, 17–27.Google Scholar

  • 14. Gączarzewicz D., Udała J., Piasecka M., Błaszczyk B., Stankiewicz T.: Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability. Turk J Biol 2015, 39, 582–594.Web of ScienceGoogle Scholar

  • 15. Jones A.R., Bubb W.A.: Substrates for endogenous metabolism by mature boar spermatozoa. J Reprod Fertil 2000, 119, 129–135.Google Scholar

  • 16. Johnson L.A., Weitze K.F., Fiser P., Maxwell W.M.: Storage of boar semen. Anim Reprod Sci 2000, 62, 143–172.CrossrefGoogle Scholar

  • 17. Manjunath P., Veronica N., Bergeron A., Menard M.: Major proteins of bovine seminal plasma bind to the low-density lipoprotein fraction of hen's egg yolk. Biol Reprod 2002, 67, 1250–1258.Google Scholar

  • 18. Medrano A., Fernández-Novell J.M., Ramió L., Álvarez J., Goldberg E., Rivera M.M., Guinovart J.J., Rigau T., Rodríguez-Gil J.E.: Utilization of citrate and lactate through a lactate deydrogenase and ATP-regulated pathway in boar spermatozoa. Mol Reprod Dev 2006, 73, 369–378.Google Scholar

  • 19. Paulenz H., Söderquist L., Pérez-Pé R., Berg K.A.: Effect of different extenders and storage temperatures on sperm viability of liquid ram semen. Theriogenology 2002, 57, 823–836.Google Scholar

  • 20. Pursel V.G., Johnson L.A., Schulman L.L.: Interaction of extender composition and incubation period on cold shock susceptibility of boar spermatozoa. J Anim Sci 1972, 35, 580–584.Google Scholar

  • 21. Rodríguez-Gil J.E., Bonet S.: Current knowledge on boar sperm metabolism: Comparison with other mammalian species. Theriogenology 2016, 85, 4–11.Web of ScienceGoogle Scholar

  • 22. Plante G., Prud'homme B., Fan J., Lafleur M., Manjunath P.: Evolution and function of mammalian binder of sperm proteins. Cell Tissue Res 2016, 363, 105–127.Web of ScienceGoogle Scholar

  • 23. Strzeżek J., Fraser L., Dziekońska A., Lecewicz M.: Complexity of technology preservation of boar semen – fundamental and applicative approaches. Reprod Domest Anim 2006, 41, 325–325.Google Scholar

  • 24. Strzeżek J., Fraser L., Lecewicz M., Strzeżek R., Dziekońska A.: Preparation for the preservation of animal semen, particularly boar. Patent PL217869-B1. 29 Aug 2014.Google Scholar

  • 25. Strzeżek J., Hopfer E.: Zinc ion-dependent protein in boar semen. I. Egg yolk precipitating activity and some biochemical properties. Anim Reprod Sci 1987, 13, 117–131.CrossrefGoogle Scholar

  • 26. Strzeżek J., Lecewicz L., Dziekońska A., Fraser L.: A simple method of extraction of lipoprotein fractions from avian egg yolk – protective effect on cooled boar semen. Theriogenology 2005, 63, 496–497.Google Scholar

  • 27. Strzeżek J., Opiłowski T., Puchalski A., Fraser L., Strzeżek R., Wiśniewska B., Wysocki P., Zasiadczyk Ł.: Pierwsze knury uzyskane po inseminacji loch nasieniem kriokonserwowanym użytkowanych w fermie zarodowej. Przegl Hod 2011, 1, 16–19.Google Scholar

  • 28. Watson P.F.: The roles of lipid and protein in the protection of ram spermatozoa at 5°C by egg yolk lipoprotein. J Reprod Fert 1981, 62, 483–492.CrossrefGoogle Scholar

  • 29. Watson P.F., Plummer J.M.: The response of boar sperm membranes to cold shock and cooling. In: Deep freezing of boar semen, edited by Johnson L.A., Larsson K., Swedish Univ Agric Sci, Uppsala, 1985, pp. 113–127.Google Scholar

  • 30. Zhang X.G., Yan G.J., Hong J.Y., Su Z.Z., Yang G.S., Li Q.W., Hu J.H.: Effects of bovine serum albumin on boar sperm quality during liquid storage at 17°C. Reprod Domest Anim 2015, 50, 263–269.CrossrefWeb of ScienceGoogle Scholar

About the article

Received: 2016-12-20

Accepted: 2017-03-09

Published Online: 2017-04-04

Published in Print: 2017-03-01


Citation Information: Journal of Veterinary Research, ISSN (Online) 2450-8608, DOI: https://doi.org/10.1515/jvetres-2017-0016.

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© 2017 Anna Dziekońska et al., published by De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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