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Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

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Detection of avian reoviruses in wild birds in Poland

Natalia Styś-Fijoł / Wojciech Kozdruń / Hanna Czekaj
Published Online: 2017-09-19 | DOI: https://doi.org/10.1515/jvetres-2017-0033


Introduction: The purpose of this study was to determine the occurrence of avian reovirus (ARV) infections in wild birds in Poland and attempt to propagate the selected ARV strains in chicken embryo kidney (CEK) cells or chicken SPF embryos. Material and Methods: The study included 192 wild birds representing 32 species, collected between 2014 and 2016. A part of the S4 segment encoding the σNS protein of avian reoviruses (ARVs) isolated from different species of wild birds from that period was amplified. Results: The presence of ARV was demonstrated in 58 (30.2%) wild birds belonging to nine orders. The isolated strains were propagated in chicken embryos by yolk sac inoculation, and CPE was induced in the infected CEK monolayer. Agar gel precipitation showed that two ARV isolates from rock pigeon and mute swan shared a common groupspecific antigen with chicken reovirus S1133. Specific products of predicted size were found in two ARV isolates from the chicken embryo passage and 13 ARVs isolated from CEK cells. Conclusion: The study indicates the high prevalence of ARV among wild birds in Poland and its possible transmission to farmed birds.

Keywords: wild birds; avian reovirus; Poland


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About the article

Received: 2017-04-10

Accepted: 2017-08-17

Published Online: 2017-09-19

Published in Print: 2017-09-26

Citation Information: Journal of Veterinary Research, Volume 61, Issue 3, Pages 239–245, ISSN (Online) 2450-8608, DOI: https://doi.org/10.1515/jvetres-2017-0033.

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© 2017. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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