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Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

4 Issues per year


IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

CiteScore 2016: 0.46

SCImago Journal Rank (SJR) 2015: 0.230
Source Normalized Impact per Paper (SNIP) 2015: 0.383

Open Access
Online
ISSN
2450-8608
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Development of a recombinant protein-based ELISA for detection of antibodies against bovine foamy virus

Magdalena Materniak-Kornas / Zbigniew Osiński
  • Departament of Hygiene of Feeding Stuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Marcin Rudzki / Jacek Kuźmak
Published Online: 2017-09-19 | DOI: https://doi.org/10.1515/jvetres-2017-0034

Abstract

Introduction: Infections with bovine foamy virus (BFV) were found in many countries but there is a lack of large-scale surveys on the prevalence of BFV among dairy cattle. The aim of this study was to develop and validate the recombinant Gag protein-based ELISA and to estimate the prevalence of antibodies against BFV. Material and Methods: Gag coding region from BFV was cloned into expression vector pT7Arg-STOP, which expressed a high level of recombinant Gag protein from E.coli. The ELISA was standardised, and the cut-off value and sensitivity and specificity of the test were calculated using a receiver operating characteristic and Bayesian estimation. Results: A total of 3,051 serum samples were tested by ELISA and 939 (30.8%) sera were recognised as positive. When Bayesian approach was used, the overall true BFV prevalence was 29.7% (95% CI: 25.9-33.4%). Conclusion: Expressed Gag protein of BFV has been used successfully as an antigen for ELISA. Eventually, this study provides basic information about the epidemiological status of infection with BFV in dairy cattle in Poland, which can be used for further studies on dissemination and transmission of BFV infection.

Keywords: cattle; bovine foamy virus; seroprevalence; Bayesian estimation

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About the article

Received: 2017-05-08

Accepted: 2017-08-18

Published Online: 2017-09-19

Published in Print: 2017-09-26


Citation Information: Journal of Veterinary Research, Volume 61, Issue 3, Pages 247–252, ISSN (Online) 2450-8608, DOI: https://doi.org/10.1515/jvetres-2017-0034.

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© 2017. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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