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Journal of Laboratory Medicine

Official Journal of the German Society of Clinical Chemistry and Laboratory Medicine

Editor-in-Chief: Schuff-Werner, Peter

Ed. by Ahmad-Nejad, Parviz / Bidlingmaier, Martin / Bietenbeck, Andreas / Conrad, Karsten / Findeisen, Peter / Fraunberger, Peter / Ghebremedhin, Beniam / Holdenrieder, Stefan / Kiehntopf, Michael / Klein, Hanns-Georg / Kohse, Klaus P. / Kratzsch, Jürgen / Luppa, Peter B. / Meyer, Alexander von / Nebe, Carl Thomas / Orth, Matthias / Röhrig-Herzog, Gabriele / Sack, Ulrich / Steimer, Werner / Weber, Thomas / Wieland, Eberhard / Winter, Christof / Zettl, Uwe K.


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Volume 40, Issue 3

Issues

Evaluation of the compatibility of Phoenix 100 and Microflex LT MALDI-TOF MS systems in the identification of routinely isolated microorganisms in the clinic microbiology laboratory

Cem Celik
  • Corresponding author
  • Faculty of Medicine, Department of Medical Microbiology, Cumhuriyet University, 58140, Sivas, Turkey, Tel.: +90 346 2582104, Fax: +90 346 2581305
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Elif Bilge Uysal / Uğur Tutar
  • Faculty of Health Sciences, Department of Nutrition and Diet, Cumhuriyet University, Sivas, Turkey
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Rahsan Erturk / Mustafa Zahir Bakici / Mustafa Gokhan Gozel
  • Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Cumhuriyet University, Sivas, Turkey
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-04-09 | DOI: https://doi.org/10.1515/labmed-2015-0105

Abstract

Background: Matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) is a quick, reliable, and efficient system for identifying microorganisms. Many centers that use the Phoenix 100 system today may adopt a MALDI-TOF MS system in the future. Our laboratory recently undertook this pivot. The present study evaluates the reproducibility of species identifications made by the Phoenix 100 and MALDI-TOF MS systems, during a period of transitioning laboratory instrumentation.

Methods: Eight hundred and twelve microbial isolates, from aerobic cultures of different clinical samples, were identified simultaneously with Phoenix 100 (Becton Dickinson, Sparks, MD, USA) and a Microflex LT MALDI-TOF MS (Bruker Daltonics, Bremen, Germany) devices.

Results: Both systems made identical species assignments for 98.9%, 92.1%, 95.1%, and 93.1% of Gram-negative isolates, catalase-positive Gram-positive cocci isolates, catalase-negative Gram-positive cocci isolates, and Candida isolates, respectively.

Conclusions: Identifications made by two instruments commonly used in microbiology laboratories, the Phoenix 100 and the Microflex LT MALDI-TOF MS, are highly consistent.

Reviewed publication

Ahmad-NejadP.GhebremedhinB.

Keywords: identification; MALDI-TOF MS; mass spectrometry; Microflex LT MALDI-TOF MS; Phoenix 100

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About the article

Received: 2015-11-20

Accepted: 2016-03-03

Published Online: 2016-04-09

Published in Print: 2016-06-01


Citation Information: LaboratoriumsMedizin, Volume 40, Issue 3, Pages 183–189, ISSN (Online) 1439-0477, ISSN (Print) 0342-3026, DOI: https://doi.org/10.1515/labmed-2015-0105.

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