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Journal of Laboratory Medicine

Official Journal of the German Society of Clinical Chemistry and Laboratory Medicine

Editor-in-Chief: Schuff-Werner, Peter

Ed. by Ahmad-Nejad, Parviz / Bidlingmaier, Martin / Bietenbeck, Andreas / Conrad, Karsten / Findeisen, Peter / Fraunberger, Peter / Ghebremedhin, Beniam / Holdenrieder, Stefan / Kiehntopf, Michael / Klein, Hanns-Georg / Kohse, Klaus P. / Kratzsch, Jürgen / Luppa, Peter B. / Meyer, Alexander von / Nebe, Carl Thomas / Orth, Matthias / Röhrig-Herzog, Gabriele / Sack, Ulrich / Steimer, Werner / Weber, Thomas / Wieland, Eberhard / Winter, Christof / Zettl, Uwe K.


IMPACT FACTOR 2018: 0.389

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2567-9430
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Volume 43, Issue 4

Issues

Detection of Salmonella, Campylobacter, Shiga toxin-producing E. coli and Shigella/EIEC by culture and a multiplex PCR panel in pediatric patients with acute diarrheal illness

Ozlem Koyuncu Ozyurt / Ana Laura Ferrandi Vilas Boas Bertocco / Larissa Alessandra Bourdeth Pereira / Luisa Paganelli Jimenes / Hatice Yazisiz / Betil Ozhak / Dilara Ogunc / Levent Donmez / Filiz Gunseren
  • Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Akdeniz University, Antalya, Turkey
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Aygen Yilmaz
  • Faculty of Medicine, Department of Pediatric Gastroenterology, Akdeniz University, Antalya, Turkey
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Gozde Ongut
Published Online: 2019-08-01 | DOI: https://doi.org/10.1515/labmed-2019-0037

Abstract

Background

Acute diarrhea is the second most common cause of child deaths worldwide. We investigated Salmonella species (spp.), Campylobacter spp., Shiga toxin-producing Escherichia coli (STEC) and Shigella spp./enteroinvasive E. coli (EIEC) in stool samples of 741 pediatric patients with acute diarrheal illness.

Methods

Between January 2017 and October 2017, 741 stool specimens were tested by the BD Max Enteric Bacterial Panel (EBP) assay and conventional culture.

Results

Campylobacter spp., Salmonella spp. and E. coli O157:H7 were detected in 10 (1.3%), 20 (2.7%) and two (0.3%) stool specimens by culture, respectively. Campylobacter spp., Salmonella spp., Shiga toxin and Shigella/EIEC were detected in 46 (6.2%), 37 (5.0%), 20 (2.7%) and three (0.4%) stool specimens by the BD Max EBP assay, respectively. The percentage of detected pathogens was 4.3% (32/741) by culture. Use of the BD Max EBP assay identified an additional 112 pathogens, thereby increasing the frequency to 15.1% (112/741). Empirically, 58 patients received antibiotics and 43 patients underwent probiotic and/or symptomatic therapy.

Conclusions

We concluded that nucleic acid amplification testing markedly improves the detection rates of bacterial stool pathogens and offers rapid results.

Reviewed publication

Ahmad-NejadP.Edited by: GhebremedhinB.

Keywords: bacterial enteric pathogens; BD Max EBP assay; culture; polymerase chain reaction (PCR)

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About the article

*Correspondence: Prof. Betil Ozhak, MD, Faculty of Medicine, Department of Medical Microbiology, Akdeniz University, Antalya, Turkey, Phone: (+90) 2422496914, Fax: (+90) 2422496903


Received: 2019-03-11

Accepted: 2019-07-08

Published Online: 2019-08-01

Published in Print: 2019-08-27


Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Journal of Laboratory Medicine, Volume 43, Issue 4, Pages 211–215, ISSN (Online) 2567-9449, ISSN (Print) 2567-9430, DOI: https://doi.org/10.1515/labmed-2019-0037.

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