Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Journal of Laboratory Medicine

Official Journal of the German Society of Clinical Chemistry and Laboratory Medicine

Editor-in-Chief: Schuff-Werner, Peter

Ed. by Ahmad-Nejad, Parviz / Bidlingmaier, Martin / Bietenbeck, Andreas / Conrad, Karsten / Findeisen, Peter / Fraunberger, Peter / Ghebremedhin, Beniam / Holdenrieder, Stefan / Kiehntopf, Michael / Klein, Hanns-Georg / Kohse, Klaus P. / Kratzsch, Jürgen / Luppa, Peter B. / Meyer, Alexander von / Nebe, Carl Thomas / Orth, Matthias / Röhrig-Herzog, Gabriele / Sack, Ulrich / Steimer, Werner / Weber, Thomas / Wieland, Eberhard / Winter, Christof / Zettl, Uwe K.


IMPACT FACTOR 2018: 0.389

CiteScore 2018: 0.22

SCImago Journal Rank (SJR) 2018: 0.156
Source Normalized Impact per Paper (SNIP) 2018: 0.089

Print
ISSN
2567-9430
See all formats and pricing
More options …
Volume 43, Issue 4

Issues

Next generation sequencing-guided anti-infective therapy of large liver abscess due to Edwardsiella tarda: a case report

Zhong-Zheng Zhu
  • Department of Oncology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, P.R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jiayi Zhu / Kexuan Zhu
  • Department of Burns and Plastic Surgery, General Hospital of Liaoning Provincial Armed Police Force of PLA, Shenyang, P.R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hui Wang
  • Department of Oncology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, P.R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Qing Xu
  • Corresponding author
  • Department of Oncology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, P.R. China
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Chong Bai
  • Corresponding author
  • Department of Respiratory and Critical Care Medicine, Changhai Hospital, The Second Military Medical University, 168 Changhai Road, Shanghai 200433, P.R. China
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2019-08-01 | DOI: https://doi.org/10.1515/labmed-2019-0070

Abstract

Background

Liver abscess is a potentially fatal disease, however identification of its causative pathogen remains challenging, which is crucial for guiding appropriate antibiotic therapy. To the best of our knowledge, the use of plasma next-generation sequencing (NGS) based pathogen analysis in patients with liver abscess has never been reported.

Case presentation

We report a case with large liver abscess due to Edwardsiella tarda that was diagnosed by the plasma-based NGS assay within 24 h. After a 2-week single agent anti-infection therapy, the patient completely recovered. A second NGS assay following anti-infection therapy failed to detect the pathogenic DNA. Follow-up magnetic resonance imaging (MRI) of the abdomen on 1 month and 3 months showed a gradually decrease in size of the liver abscess, and the 6-month MRI showed that the abscess disappeared completely.

Conclusions

Our data indicated that the plasma NGS test can sensitively and minimal-invasively detect pathogens within a clinically actionable timeframe, and antibiotic alone is an alternative to first-line treatment for large liver abscess with the aid of NGS for accurate detection of the pathogenic bacterial genome.

Keywords: Edwardsiella tarda; liver abscess; next generation sequencing

References

  • 1.

    Mischnik A, Kern WV, Thimme R. Pyogenic liver abscess: changes of organisms and consequences for diagnosis and therapy. Dtsch Med Wochenschr 2017;142:1067–74.PubMedWeb of ScienceGoogle Scholar

  • 2.

    Lok KH, Li KF, Li KK, Szeto ML. Pyogenic liver abscess: clinical profile, microbiological characteristics, and management in a Hong Kong hospital. J Microbiol Immunol Infect 2008;41:483–90.Google Scholar

  • 3.

    Zhu X, Wang S, Jacob R, Fan Z, Zhang F, Ji G. A 10-year retrospective analysis of clinical profiles, laboratory characteristics and management of pyogenic liver abscesses in a chinese hospital. Gut Liver 2011;5:221–7.CrossrefWeb of ScienceGoogle Scholar

  • 4.

    Du ZQ, Zhang LN, Lu Q, Ren YF, Lv Y, Liu XM, et al. Clinical charateristics and outcome of pyogenic liver abscess with different size: 15-year experience from a single center. Sci Rep 2016;6:35890.CrossrefPubMedWeb of ScienceGoogle Scholar

  • 5.

    Liu L, Chen W, Lu X, Zhang K, Zhu C. Pyogenic liver abscess: a retrospective study of 105 cases in an emergency department from East China. J Emerg Med 2017;52:409–16.CrossrefWeb of ScienceGoogle Scholar

  • 6.

    Li GY, Li J, Xiao P, Guo YH, Mo ZL. Detection of type III secretion gene as an indicator for pathogenic Edwardsiella tarda. Lett Appl Microbiol 2011;52:213–9.CrossrefPubMedWeb of ScienceGoogle Scholar

  • 7.

    Suzuki K, Yanai M, Hayashi Y, Otsuka H, Kato K, Soma M. Edwardsiella tarda bacteremia with psoas and epidural abscess as a food-borne infection: a case report and literature review. Intern Med 2018;57:893–7.Web of ScienceCrossrefPubMedGoogle Scholar

  • 8.

    Janda JM, Abbott SL. Infections associated with the genus Edwardsiella: the role of Edwardsiella tarda in human disease. Clin Infect Dis 1993;17:742–8.PubMedCrossrefGoogle Scholar

  • 9.

    Yousuf RM, How SH, Amran M, Hla KT, Shah A, Francis A. Edwardsiella tarda septicemia with underlying multiple liver abscesses. Malays J Pathol 2006;28:49–53.PubMedGoogle Scholar

  • 10.

    Ebisawa KF, Nishimura S, Yamamoto S, Ohji G, Iwata K. Mycotic aneurysm caused by Edwardsiella tarda successfully treated with stenting and suppressive antibiotic therapy: a case report and systematic review. Ann Clin Microbiol Antimicrob 2018;17:21.CrossrefWeb of SciencePubMedGoogle Scholar

  • 11.

    Hirai Y, Asahata-Tago S, Ainoda Y, Fujita T, Kikuchi K. Edwardsiella tarda bacteremia. A rare but fatal water- and foodborne infection: review of the literature and clinical cases from a single centre. Can J Infect Dis Med Microbiol 2015;26:313–8.CrossrefPubMedWeb of ScienceGoogle Scholar

  • 12.

    Watanabe N, Kryukov K, Nakagawa S, Takeuchi JS, Takeshita M, Kirimura Y, et al. Detection of pathogenic bacteria in the blood from sepsis patients using 16S rRNA gene amplicon sequencing analysis. PLoS One 2018;13:e0202049.PubMedWeb of ScienceCrossrefGoogle Scholar

  • 13.

    Frickmann H, Kunne C, Hagen RM, Podbielski A, Normann J, Poppert S, et al. Next-generation sequencing for hypothesis-free genomic detection of invasive tropical infections in poly-microbially contaminated, formalin-fixed, paraffin-embedded tissue samples – a proof-of-principle assessment. BMC Microbiol 2019;19:75.CrossrefPubMedWeb of ScienceGoogle Scholar

  • 14.

    Liu P, Weng X, Zhou J, Xu X, He F, Du Y, et al. Next generation sequencing based pathogen analysis in a patient with neurocysticercosis: a case report. BMC Infect Dis 2018;18:113.CrossrefWeb of ScienceGoogle Scholar

  • 15.

    Hope WW, Vrochides DV, Newcomb WL, Mayo-Smith WW, Iannitti DA. Optimal treatment of hepatic abscess. Am Surg 2008;74:178–82.PubMedGoogle Scholar

About the article

Corresponding authors: Dr. Qing Xu, Department of Oncology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai 200072, P.R. China, Phone: +86 21 66313384, Fax: +86 21 66313384 and Dr. Chong Bai Department of Respiratory and Critical Care Medicine, Changhai Hospital, The Second Military Medical University, 168 Changhai Road, Shanghai 200433, P.R. China, Phone: +86-21-3116-1312, Fax: +86-21-3116-1312


Received: 2019-04-28

Accepted: 2019-06-26

Published Online: 2019-08-01

Published in Print: 2019-08-27


Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Journal of Laboratory Medicine, Volume 43, Issue 4, Pages 210–213, ISSN (Online) 2567-9449, ISSN (Print) 2567-9430, DOI: https://doi.org/10.1515/labmed-2019-0070.

Export Citation

© 2019 Walter de Gruyter GmbH, Berlin/Boston.Get Permission

Comments (0)

Please log in or register to comment.
Log in