Jump to ContentJump to Main Navigation
Show Summary Details

Macedonian Veterinary Review

The Journal of the Faculty of Veterinary Medicine-Skopje at the Ss. Cyril and Methodius University in Skopje

2 Issues per year


SCImago Journal Rank (SJR) 2015: 0.203
Source Normalized Impact per Paper (SNIP) 2015: 0.565
Impact per Publication (IPP) 2015: 0.325

Open Access
Online
ISSN
1857-7415
See all formats and pricing

Hematological and Biochemical Parameters in Symptomatic and Asymptomatic Leishmania-Seropositive Dogs

Igor Ulchar
  • Corresponding author
  • Department of Pathophysiology, Faculty of Veterinary Medicine – Skopje, Ss Cyril and Methodius University, Skopje
  • Email:
/ Irena Celeska
  • Department of Pathophysiology, Faculty of Veterinary Medicine – Skopje, Ss Cyril and Methodius University, Skopje
/ Jovana Stefanovska
  • Department of Parasitology, Faculty of Veterinary Medicine – Skopje, Ss Cyril and Methodius University, Skopje
/ Anastasija Jakimovska
  • Department of Pathophysiology, Faculty of Veterinary Medicine – Skopje, Ss Cyril and Methodius University, Skopje
Published Online: 2015-11-18 | DOI: https://doi.org/10.14432/j.macvetrev.2015.06.04

Abstract

Leishmaniosis caused by Leishmania infantum is vector-born severe enzootic disease in dogs. It includes a wide spectrum of clinical symptoms, but the most characteristic are alterations in the hematopoetic system and renal failure. Also, infected animals could be asymptomatic, so the manifestation of L. infantum infection depends on many factors, including host’s immunological status. The aim of this survey was to find parameters related with hematopoetic and renal failure (hematology, biochemical parameters - urea, creatinine, serum proteins) in symptomatic and asymptomatic dogs seropositive for canine leishmaniosis. Within the hematological parameters, we found significant differences between symptomatic and asymptomatic dogs in the erythrogram and platelet count, but not in the leukogram. Significant differences between the two groups were found also for urea, creatinine, serum albumin and globulin, but not in serum total protein and A/G ratio. These findings indicate individual variability of the host’s response to infection with L. infantum.

Keywords: leishmaniosis; dog; hematology; biochemistry

References

  • 1. Solano-Gallego, L., Koutinas, A., Miró, G., Cardoso, L., Pennisi, M.G., Ferrer, L., Bourdeau, P., Oliva, G., Baneth, G. (2009). Directions for the diagnosis, clinical staging, treatment and prevention of canine leishmaniosis. Veterinary Parasitology 165, 1-18. http://dx.doi.org/10.1016/j.vetpar.2009.05.022 PMid:19559536 [Web of Science] [Crossref]

  • 2. Gonçalves, R., Vieira, E.R., Melo, M.N, Gollob, K.J., Mosser, D.M, Tafuri, W.L. (2005). A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infectious Diseases, 5, 39. http://dx.doi.org/10.1186/1471-2334-5-39 PMid:15913461 PMCid:PMC1166554 [Crossref]

  • 3. Dias, E.L., Batista, Z.S., Guerra, R.M.S.N.C., Calabrese, K.S., Lima, T.B., Abreu-Silva, A.L. (2008). Canine Visceral Leishmaniosis (CVL): Seroprevalence, clinical, hematological and biochemical findings of dogs naturally infected in an endemic area of São José de Ribamar Municipality, Maranhão State, Brazil. Ciência animal brasileira, 9(3): 740-745.

  • 4. Bates, P.A. (2007). Transmission of Leishmania metacyclic promastigotes by phlebotomine sand flies. Int J Parasitol. 37 (10-3): 1097-1106. http://dx.doi.org/10.1016/j.ijpara.2007.04.003 PMid:17517415 PMCid:PMC2675784 [Crossref]

  • 5. Dostálová, A, Volf, P. (2012). Leishmania development in sand flies: parasite-vector interactions overview. Parasites & Vectors 5, 276. http://dx.doi.org/10.1186/1756-3305-5-276 PMid:23206339 PMCid:PMC3533922 [Crossref] [Web of Science]

  • 6. Gramiccia, M., Gradoni, L. (2005). The current status of zoonotic leishmaniases and approaches to disease control. Int J Parasitol. 35, 1169-1180. http://dx.doi.org/10.1016/j.ijpara.2005.07.001 PMid:16162348 [Crossref]

  • 7. Sakamoto, K.P., de Melo, G.D., Machado, G.F. (2013). T and B lymphocytes in the brains of dogs with concomitant seropositivity to three pathogenic protozoans: Leishmania chagasi, Toxoplasma gondii and Neospora caninum. BMC Research Notes 6, 226. http://dx.doi.org/10.1186/1756-0500-6-226 PMid:23758819 PMCid:PMC3701587 [Crossref]

  • 8. Sanches, F.P., Tomokane, T.Y, Da Matta, V.L.R., Marcondes, M., Corbett, C.E.P., Laurenti, M.D. (2014). Expression of inducible nitric oxide synthase in macrophages inversely correlates with parasitism of lymphoid tissues in dogs with visceral leishmaniosis. Acta Vet. Scand. 56, 57. http://dx.doi.org/10.1186/s13028-014-0057-z PMid:25195062 PMCid:PMC4172852 [Web of Science] [Crossref]

  • 9. Verçosa, B.L.A., Lemos, C.M., Mendonça, I.L., Silva, S.M.M.S., de Carvalho, S.M., Goto, H., Costa, F.A.L. (2008). Transmission potential, skin inflammatory response, and parasitism of symptomatic and asymptomatic dogs with visceral leishmaniosis. BMC Veterinary Research 4, 45. http://dx.doi.org/10.1186/1746-6148-4-45 PMid:18990238 PMCid:PMC2613136 [Crossref] [Web of Science]

  • 10. López-Peña, M., Alemañ, N., Muñoz, F., Fondevila, D., Suárez, M.L., Goicoa, A., Nieto, J.M. (2009). Visceral leishmaniosis with cardiac involvement in a dog: a case report. Acta Vet. Scand., 51, 20. http://dx.doi.org/10.1186/1751-0147-51-20 PMid:19405946 PMCid:PMC2679027 [Web of Science] [Crossref]

  • 11. Manna, L., Paciello, O., Della Morte, R., Gravino, A.E. (2012). Detection of Leishmania parasites in the testis of a dog affected by orchitis: case report. Parasit Vectors 5, 216. http://dx.doi.org/10.1186/1756-3305-5-216 PMid:23021706 PMCid:PMC3481428 [Crossref]

  • 12. McConkey, S.E., López, A., Shaw, D., Calder, J. (2002). Leishmanial polyarthritis in a dog. Can Vet J., 43 (8): 607-609. PMid:12170836 PMCid:PMC339396

  • 13. Melo, F.A., Moura, E.P., Ribeiro, R.R, Alves, C.F., Caliari, M.V., Tafuri, W.L., Calabrese, K.S., Tafuri, W.L. (2009). Hepatic extracellular matrix alterations in dogs naturally infected with Leishmania (Leishmania) chagasi. Int. J. Exp. Path. 90 (5): 538-548. http://dx.doi.org/10.1111/j.1365-2613.2009.00681.x PMid:19765108 PMCid:PMC2768152 [Crossref]

  • 14. Silva, L.C., Castro, R.S, Figueiredo, M.M., Michalick, M.S.M., Tafuri, W.L., Tafuri, W.L. (2013). Canine visceral leishmaniosis as a systemic fibrotic disease. Int. J. Exp. Path. 94 (2): 133-143. http://dx.doi.org/10.1111/iep.12010 PMid:23419132 PMCid:PMC3607142 [Crossref]

  • 15. Figueiredo, M.M., Deoti, B., Amorim, I.F., Pinto, A.J.W., Moraes, A., Carvalho, C.S., da Silva, S.M., de Assis, A.C. B., de Faria, A.M.C., Tafuri, W.L. (2014). Expression of regulatory T cells in jejunum, colon, and cervical and mesenteric lymph nodes of dogs naturally infected with Leishmania infantum. Infect Immun., 82 (9): 3704-3712. http://dx.doi.org/10.1128/IAI.01862-14 PMid:24935975 PMCid:PMC4187817 [Web of Science] [Crossref]

  • 16. Pinto, A.J.W., Figueiredo, M.M., Silva, F.L., Martins, T., Michalick, M.S.M., Tafuri, W.L., Tafuri, W.L. (2011). Histopathological and parasitological study of the gastrointestinal tract of dogs naturally infected with Leishmania infantum. Acta Vet Scand, 53, 67. http://dx.doi.org/10.1186/1751-0147-53-67 PMid:22166041 PMCid:PMC3269393 [Crossref] [Web of Science]

  • 17. Viegas, C., Requicha, J., Albuquerque, C., Sargo T., Machado J., Dias I., Pires, M.A., Campino, L., Cardoso, L. (2012). Tongue nodules in canine leishmaniosis - a case report. Parasit Vectors 5, 120. http://dx.doi.org/10.1186/1756-3305-5-120 PMid:22704596 PMCid:PMC3407507 [Crossref]

  • 18. Bogdan, C., Röllinghoff, M. (1998). The immune response to Leishmania: mechanisms of parasite control and evasion. Int J Parasitol, 28 (1): 121-134. http://dx.doi.org/10.1016/S0020-7519(97)00169-0 [Crossref]

  • 19. Loría-Cervera, E.N., Andrade-Narváez, F.J. (2014). Animal Models for the Study of Leishmaniosis Immunology. Rev. Inst. Med. Trop. Sao Paulo, 56 (1): 1-11. http://dx.doi.org/10.1590/S0036-46652014000100001 PMid:24553602 PMCid:PMC4085833 [Crossref]

  • 20. Pinelli, E., Killick-Kendrick, R., Wagenaar, J., Bernadina, W., del Real, G., Ruitenberg, J. (1994). Cellular and humoral immune responses in dogs experimentally and naturally infected with Leishmania infantum. Infect Immun., 62 (1): 229-235. PMid:8262632 PMCid:PMC186091

  • 21. Pinelli, E., Rutten, V.P.M.G., Bruysters, M., Moore, P.F., Ruitenberg, E.J. (1999). Compensation for decreased expression of B7 molecules on Leishmania infantuminfected canine macrophages results in restoration of parasite-specific T-cell proliferation and gamma interferon production. Infect Immun., 67 (1): 237-243. PMid:9864221 PMCid:PMC96302

  • 22. do Nascimento, P.R.P., Martins, D.R.A., Monteiro, G.R.G., Queiroz, P.V., Freire-Neto, F.P., Queiroz, J.W., Lima, Á.L.M., Jeronimo, S.M.B. (2013). Association of pro-inflammatory cytokines and iron regulatory protein 2 (IRP2) with Leishmania burden in canine visceral leishmaniosis. PLoS One 8(10): e73873. http://dx.doi.org/10.1371/journal.pone.0073873 [Crossref]

  • 23. Souza, C.C., Barreto, T.O., da Silva, S.M., Pinto, A.W. J., Figueiredo, M.M., Rocha, O.G.F., Cangussú, S.D., Tafuri, W.L. (2014). A potential link among antioxidant enzymes, histopathology and trace elements in canine visceral leishmaniosis. Int. J. Exp. Path., 95 (4): 260-270. http://dx.doi.org/10.1111/iep.12080 PMid:24766461 PMCid:PMC4170968 [Crossref]

  • 24. Coura-Vital, W., Marques, M.J., Giunchetti, R.C., Teixeira-Carvalho, A., Moreira, N.D., Vitoriano-Souza, J., Vieira, P.M., Carneiro, C.M., Corrêa-Oliveira, R., Martins-Filho, O.A., Carneiro, M., Reis, A.B. (2011). Humoral and cellular immune responses in dogs with inapparent natural Leishmania infantum infection. Vet J. 190 (2), e43-e47. http://dx.doi.org/10.1016/j.tvjl.2011.04.005 PMid:21596598 [Crossref]

  • 25. Nicolato, R.deC., de Abreu, R.T., Roatt, B.M., Aguiar-Soares, R.D.O., Reis, L.E.S., Carvalho, M.G., Carneiro, C.M., Giunchetti, R.C., Bouillet, L.E.M., Lemos, D.S, Coura-Vital, W., Reis, A.B. (2013). Clinical forms of canine visceral leishmaniosis in naturally leishmania infantum-infected dogs and related myelogram and hemogram changes. PLoS One. 8 (12): e82947. http://dx.doi.org/10.1371/journal.pone.0082947 [Crossref]

  • 26. Kargin Kiral, F., Seyrek, K., Pasa, S., Ertabaklar, H., Ünsal, C. (2004). Some haematological, biochemical and electrophoretic findings in dogs with visceral leishmaniosis. Revue Méd. Vét., 155 (4): 226-229.

  • 27. Honse, C.O., Figueiredo, F.B., de Alencar, N.X., Madeira, M.deF., Gremião, I.D.F, Schubach, T.M.P. (2013). Disseminated intravascular coagulation in a dog naturally infected by Leishmania (Leishmania) chagasi from Rio de Janeiro - Brazil. BMC Veterinary Research 9, 43. http://dx.doi.org/10.1186/1746-6148-9-43 PMid:23497531 PMCid:PMC3599858 [Crossref] [Web of Science]

  • 28. Costa, F.A.L., Prianti, M.G., Silva, T.C., Silva, S.M.M.S., Guerra, J.L., Goto, H. (2010). T cells,tadhesion molecules and modulation of apoptosis in visceral leishmaniosis glomerulonephritis. BMC Infect Dis., 10, 112. http://dx.doi.org/10.1186/1471-2334-10-112 [Crossref]

  • 29. Pateraki, E., Portocala, R., Labrousse, H., Guesdon, J.-L. (1983). Antiactin and antitubulin antibodies in canine visceral leishmaniosis. Infect Immun. 42 (2): 496-500. PMid:6642639 PMCid:PMC264456

  • 30. Menn, B., Lorentz, S., Naucke, T.J. (2010). Imported and travelling dogs as carriers of canine vector-borne pathogens in Germany. Parasit Vectors, 3, 34. http://dx.doi.org/10.1186/1756-3305-3-34 PMid:20377872 PMCid:PMC2857866 [Web of Science] [Crossref]

  • 31. Momo, C., Jacintho, A.P.P., Moreira, P.R.R., Munari, D.P., Machado, G.F., Vasconcelos, R.deO. (2014). Morphological changes in the bone marrow of the dogs with visceral leishmaniosis. Vet Med Int., 150582. http://dx.doi.org/10.1155/2014/150582 PMid:24744957 PMCid:PMC3972870 [Crossref]

  • 32. Živičnjak, T., Martinković, F., Marinculić, A., Mrljak, V., Kučer, N., Matijatko, V., Mihaljević, Ž., Barić-Rafaj, R. (2005). A seroepidemiologic survey of canine visceral leishmaniosis among apparently healthy dogs in Croatia. Vet Parasitol. 131 (1-2): 35-43. http://dx.doi.org/10.1016/j.vetpar.2005.04.036 PMid:15946800 [Crossref]

  • 33. Office International des Epizooties (OIE) (2008). Manual of standards diagnostic tests and vaccines, Part 2, Section 2.2, Chapter 2.2.11.: Leishmaniosis.

  • 34. Solano-Gallego, L., Miró, G., Koutinas, A., Cardoso, L., Pennisi, M.G., Ferrer, L., Bourdeau, P., Oliva, G., Baneth, G. (2011). LeishVet guidelines for the practical management of canine leishmaniosis. Parasites & Vectors 4, 86. http://dx.doi.org/10.1186/1756-3305-4-86 PMid:21599936 PMCid:PMC3125381 [Web of Science] [Crossref]

  • 35. Trópia de Abreu, R., Carvalho, M.dG., Carneiro, C.M., Giunchetti, R.C., Teixeira-Carvalho, A., Martins-Filho, O.A., Coura-Vital, W., Corrêa-Oliveira, R., Reis, A.B. (2011). Influence of clinical status and parasite load on erythropoiesis and leucopoiesis in dogs naturally infected with Leishmania (Leishmania) chagasi. PLoS ONE 6 (5): e18873. http://dx.doi.org/10.1371/journal.pone.0018873 [Web of Science] [Crossref]

  • 36. De Tommasi, A.S., Otranto, D., Furlanello, T., Tasca, S., Cantacessi, C., Breitschwerdt, E.B., Stanneck, D., Dantas-Torres, F., Baneth, G., Capelli, G., de Caprariis, D. (2014). Evaluation of blood and bone marrow in selected canine vector-borne diseases. Parasit Vectors. 7, 534. http://dx.doi.org/10.1186/s13071-014-0534-2 PMid:25441458 PMCid:PMC4261574 [Crossref]

  • 37. Ramos-Vara, J.A., Briones, V., Segalés, J., Vilafranca, M., Sordé, A., Miller, M.A. (1994). Concurrent infection with Streptococcus equisimilis and Leishmania in a dog. J Vet Diagn Invest., 6 (3): 371-375. http://dx.doi.org/10.1177/104063879400600317 [Crossref]

  • 38. Mekuzas, Y., Gradoni, L., Oliva, G., Foglia Manzillo, V., Baneth, G. (2009). Ehrlichia canis and Leishmania infantum co-infection: a 3-year longitudinal study in naturally exposed dogs. Clin Microbiol Infect., 15 (Suppl. 2): 30-31. http://dx.doi.org/10.1111/j.1469-0691.2008.02150.x PMid:19416288 [Web of Science]

About the article

Received: 2015-04-16

Revised: 2015-05-22

Accepted: 2015-05-28

Published Online: 2015-11-18

Published in Print: 2015-10-01


Citation Information: Macedonian Veterinary Review, ISSN (Online) 1857-7415, DOI: https://doi.org/10.14432/j.macvetrev.2015.06.04. Export Citation

© 2015. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. (CC BY-NC-ND 4.0)

Comments (0)

Please log in or register to comment.
Log in