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Macedonian Veterinary Review

The Journal of the Faculty of Veterinary Medicine-Skopje at the Ss. Cyril and Methodius University in Skopje

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SCImago Journal Rank (SJR) 2015: 0.203
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Online
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1857-7415
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Phenotypic and Genotypic Characteristics of Enterocin Producing Enterococci against Pathogenic Bacteria

Sandra Mojsova
  • Corresponding author
  • Food Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Republic of Macedonia
  • Email:
/ Kiril Krstevski
  • Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Republic of Macedonia
/ Igor Dzadzovski
  • Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Republic of Macedonia
/ Zagorka Popova
  • Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Republic of Macedonia
/ Pavle Sekulovski
  • Food Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Republic of Macedonia
Published Online: 2015-11-18 | DOI: https://doi.org/10.14432/j.macvetrev.2015.08.052

Abstract

The study investigated the antimicrobial activity of 13 enterococcal strains (E. faecalis -8, E. faecium-2, E. hirae-2, E. spp.-1) isolated from our traditional cheeses against pathogen microorganisms. Also, it includes the detection of the following enterocin structural genes: enterocin A, enterocin B, enterocin P, enterocin L50A/B, bacteriocin 31, enterocin AS48, enterocin Q, enterocin EJ97 and cytolysin by using PCR method. All isolates inhibited growth of L. monocytogenes and L.innocua. One isolate had a broader antimicrobial activity. None of the isolates showed inhibitory activity against S. enteritidis, E. coli and Y. enterocolitica. The genes enterocin P, cytolysin and enterocin A were the most frequently detected structural genes among the PCR positive strains. No amplification was obtained in two strains E. faecalis-25 and E. faecalis-86. Three different genes were identified in some strains. With the exclusion of strains possessing a virulence factor, such as cytolysin, producers of more than one enterocins could be of a great technological potential as protective cultures in the cheese industry.

Keywords: traditional cheese; enterococci; enterocins; antimicrobial activity

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About the article

Received: 2015-06-15

Revised: 2015-07-28

Accepted: 2015-08-07

Published Online: 2015-11-18

Published in Print: 2015-10-01


Citation Information: Macedonian Veterinary Review, ISSN (Online) 1857-7415, DOI: https://doi.org/10.14432/j.macvetrev.2015.08.052. Export Citation

© 2015. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. (CC BY-NC-ND 4.0)

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