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Macedonian Veterinary Review

The Journal of the Faculty of Veterinary Medicine-Skopje at the Ss. Cyril and Methodius University in Skopje

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Online
ISSN
1857-7415
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The Prevalence of Pasteurella Multocida from Farm Pigs in Serbia

Oliver Radanovic
  • Corresponding author
  • Institute of Veterinary Medicine of Serbia, Vojvode Toze 14, 11000 Belgrade, Serbia
  • Email:
/ Jadranka Zutic
  • Institute of Veterinary Medicine of Serbia, Vojvode Toze 14, 11000 Belgrade, Serbia
/ Dobrila Jakic-Dimic
  • Institute of Veterinary Medicine of Serbia, Vojvode Toze 14, 11000 Belgrade, Serbia
/ Branislav Kureljusic
  • Institute of Veterinary Medicine of Serbia, Vojvode Toze 14, 11000 Belgrade, Serbia
/ Bozidar Savic
  • Institute of Veterinary Medicine of Serbia, Vojvode Toze 14, 11000 Belgrade, Serbia
Published Online: 2016-03-14 | DOI: https://doi.org/10.1515/macvetrev-2015-0063

Abstract

The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37°C and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of Pasteurella multocida, the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of Pasteurella multocida were obtained from 71 (35 %) samples. Out of the remaining 29 (14%) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of Pasteurella multocida and Streptococcus spp., Arcanobacterium pyogenes, Actinobacillus pleuropneumoniae and Haemophilus parasuis, respectively. The PCR method confirmed that all 15 investigated strains of P. multocida belong to type A.

Keywords: Pasteurella multocida; lung; pig

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About the article


Received: 2015-07-20

Revised: 2015-10-16

Accepted: 2015-10-19

Published Online: 2016-03-14

Published in Print: 2016-03-01


Citation Information: Macedonian Veterinary Review, ISSN (Online) 1857-7415, DOI: https://doi.org/10.1515/macvetrev-2015-0063. Export Citation

© 2015 Oliver Radanovic et al., published by De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

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